In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis
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Leishmaniasis causes considerable mortality throughout the world, affecting more than 12 million people. Cymbopogon citratus (DC) Stapf, Family Poaceae, is a widely used herb in tropical countries and is also known as a source of ethnomedicines. In this study, the inhibitory effect and the morphological and ultrastructural alterations on Leishmania amazonensis by the essential oil (EO) of C. citratus and its main constituent, citral, were evaluated. The results showed that the antiproliferative activity of EO on promastigotes and axenic amastigotes, and intracellular amastigote forms of L. amazonensis was significantly better than citral, and indicated a dose-dependent effect. Neither compound showed a cytotoxic effect on macrophage strain J774G8. The promastigote forms of L. amazonensis underwent remarkable morphological and ultrastructural alterations compared with untreated cultures. These alterations were visible by light, scanning, and transmission electron microscopy of promastigotes treated with EO and citral at concentrations corresponding to the IC50 (1.7 and 8.0 µg/ml) and IC90 (3.2 and 25 µg/ml), respectively, after 72 h of incubation. This study revealed that citral-rich essential oil from C. citratus has promising antileishmanial properties, and is a good candidate for further research to develop a new anti-protozoan drug.
KeywordsLeishmaniasis Cutaneous Leishmaniasis Geranial Antileishmanial Activity Amastigote Form
This study was supported through grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, Capacitação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES, Financiadora de Estudos e Projetos—FINEP, and Programa de Pós-graduação em Ciências Farmacêuticas da Universidade Estadual de Maringá.
- Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry; 4th edn. Allured, Carol Stream, Illinois, p 804Google Scholar
- Kanko C, Sawaliho E, Kone S, Koukoua G, N'guessan YT (2004) Étude des propriétés physico-chimiques des huiles essentielles de Lippia multiflora, Cymbopogon citratus, Cymbopogon nardus, Cymbopogon giganteus. C R Chim 7:1039–1049Google Scholar
- Luize PS, Tiuman TS, Morello LG, Maza PK, Ueda-Nakamura T, Filho BPD, Cortez DAG, Mello JCP, Nakamura CV (2005) Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Braz J Pharm Sci 41:85–94Google Scholar
- Melo SF, Soares SF, Costa RF, Silva CR, Oliveira MBN, Bezerra RJAC, Caldeira-de-Araujo A, Bernardo-Filho M (2001) Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli. Mutat Res 496:33–38PubMedGoogle Scholar
- Pedroso RB, Ueda-Nakamura T, Dias Filho BP, Cortez DAG, Cortez LER, Morgado-Díaz JA, Nakamura CV (2006) Biological activities of essential oil obtained from Cymbopogon citratus on Crithidia deanei. Acta Protozool 45:231–240Google Scholar
- Puatanachokchai R, Kishida H, Denda A, Murata N, Konishi Y, Vinitketkumnuen U, Nakae D (2002) Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats. Cancer Lett 183:9–15CrossRefPubMedGoogle Scholar
- Rodrigues JCF, Attias M, Rodriguez C, Urbina JA, Souza W (2002) Ultrastructural and biochemical alterations induced by 22, 26-azasterol, a Δ24(25)-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrob Agents Chemother 46:487–499CrossRefPubMedGoogle Scholar
- Santoro GF, Cardoso MG, Guimarães LGL, Freire JM, Soares MJ (2007) Anti-proliferative effect of the essential oil of Cymbopogon citratus (DC) Stapf (lemongrass) on intracellular amastigotes, bloodstream trypomastigotes and culture epimastigotes of Trypanosoma cruzi (Protozoa: Kinetoplastida). Parasitol 134:1649–1656Google Scholar
- WHO - WORLD HEALTH ORGANIZATION. Leishmaniasis: disease burden and epidemiological trends. Special Programme for Research and Training in Tropical Diseases. Geneva: WHO, 2002. Online:http://www.who.int/tdr/diseases/leish/files/direction.pdf. Accessed 11 december 2007