Parasitology Research

, 105:1185 | Cite as

An update on metacercarial excystment of trematodes

Review
First Online:
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Accepted:

Abstract

This review updates an earlier one by Fried (Adv Parasitol 33:91–144, 1994) on metacercarial excystation of trematodes. Our coverage of the salient literature is mainly from 1995 to 2008 and includes significant studies on echinostomatid and non-echinostomatid trematodes. Most of the echinostomatid studies are on species of 37-collar spined Echinostoma, although one study is concerned with a species of Echinochasmus. The non-echinostomatid coverage includes work on species of Paragonimus, Haplorchis, Apatemon, Microphallus, Clonorchis, Riberoia, Maritema, Opisthorchis, and Zygocotyle. Most of the studies were concerned with methods used to excyst the metacercariae of the species being investigated. However, some studies were concerned with metacercarial excystation in order to use the excysted larvae for in vitro cultivation or morphometric studies. Other studies used the excysted larvae for physiological, biochemical, or behavioral work. Several studies were also concerned with elucidating both intrinsic and extrinsic factors which mediate chemical excystation of the metacercarial stage of digenetic trematodes

Keywords

Bile Salt Nicardipine Chloromethyl Ketone Fluorogenic Peptide Substrate Metacercarial Cyst 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Bennett SC, Irwin SWB, Fitzpatrick SM (2003) Tributyltin and copper effects on encystment and in vitro excystment of Parorchis acanthus larvae. J Helminthol 77:291–296CrossRefPubMedGoogle Scholar
  2. Chung YB, Kong Y, Joo IJ, Cho SY, Kang SY (1995) Excystment of Paragonimus westermani by endogenous cysteine protease. J Parasitol 81:137–142CrossRefPubMedGoogle Scholar
  3. El Mayas H, Kearn GC (1995) In vitro excystment of the metacercaria of Cryptocotyle convavum from the common goby Pomatoschistus micrcops. J Helminthol 69:285–297CrossRefGoogle Scholar
  4. Fredensborg BL, Poulin R (2005) In vitro cultivation of Maritrema novaezealandensis (Microphallidae): the effect of culture medium on excystation, survival and egg production. Parasitol Res 95:310–313CrossRefPubMedGoogle Scholar
  5. Fried B (1994) Metacercarial excystment of trematodes. Adv Parasitol 33:92–120Google Scholar
  6. Fried B, Roth RM (1974) In vitro excystment of the metacercaria of Parorchis acanthus. J Parasitol 60:465CrossRefPubMedGoogle Scholar
  7. Fried B, Reddy A (1997) Comparative studies on excystation and morphological features of the metacercariae of Echinostoma paraensei and E. caproni. Int J Parasitol 27:899–901CrossRefPubMedGoogle Scholar
  8. Fried B, LaTerra R (2002) In vitro and in vivo encystment of the cercariae of Echinostoma caproni. J Parasitol 88:1124–1129PubMedGoogle Scholar
  9. Fried B, Schneck JL (2004) Effects of copper sulphate on in vitro encystment of the cercariae of Echinostoma caproni. J Helminthol 78:371–372CrossRefPubMedGoogle Scholar
  10. Fried B, Johnson PTJ (2005) In vitro excystment of the metacercariae of Ribeiroia ondatrae. Parasitol Res 95:293–295CrossRefPubMedGoogle Scholar
  11. Fried B, Peoples RC (2007a) Viability of the encysted metacercariae of Echinostoma caproni judged by light microscopy versus chemical excystation. J Parasitol 93:947CrossRefPubMedGoogle Scholar
  12. Fried B, Peoples RC (2007b) Effects of various physical and chemical factors on excystation of the encysted metacercariae of Echinostoma caproni. J Parasitol 93:1388–1391CrossRefPubMedGoogle Scholar
  13. Fried B, Peoples RC (2009) Maintenance, cultivation, and excystation of echinostomes: 2000–2007. In: Fried B, Toledo R (eds) The biology of echinostomes. Springer, New York, pp 112–124Google Scholar
  14. Fried B, Schmidt KA, Sorensen RE (1997) In vivo and ectopic encystment of Echinostoma revolutum and chemical excystation of the metacercariae. J Parasitol 83:251–254CrossRefPubMedGoogle Scholar
  15. Fried B, Reddy A, Balfour CD (2001) Excystation and distribution of metacercariae of Echinostoma caproni in ICR mice. Comp Parasitol 68:259–261Google Scholar
  16. Fried B, Schneck JL, Ponder EL (2004) Effects of tonicity, digestive enzymes and bile salts, and nutrient media on the survival of excysted metacercariae of Echinostoma caproni. Parasitol Res 93:527–530Google Scholar
  17. Humphries JE, Fried B (1996) Histological and histochemical studies on the paraoesophageal glands in cercariae and metacercariae of Echinostoma revolutum and E. trivolvis. J Helminthol 70:299–301CrossRefGoogle Scholar
  18. Ikeda T (2006) Effects of L-type Ca2+ channel antagonists on in vitro excystment of Paragonimus ohirai metacercariae induced by sodium cholate. Parasitol Res 99:336–340CrossRefPubMedGoogle Scholar
  19. Intapan PM, Maleewong W (2001) In vitro excystation of Paragonimus heterotremus metacercariae. J Parasitol 87:1184–1186PubMedGoogle Scholar
  20. Irwin SWB, McKerr G, Judge BC, Moran I (1984) Studies on metacercarial excystment in Himasthla leptosoma (Trematoda: Echinostomatidae) and newly emerged metacercariae. Int J Parasitol 14:415–421CrossRefGoogle Scholar
  21. Kaewpitoon N, Laha T, Kaewkes S, Yongvanit P, Brindley PJ, Loukas A, Sripa B (2008) Characterization of cysteine proteases from the carcinogenic liver fluke, Opisthorchis viverrini. Parasitol Res 102:757–764CrossRefPubMedGoogle Scholar
  22. Khan RN, Pacut JR, Pung OJ, Lawson V, Marshall M (2003) In vitro excystation of metacercariae of Microphallus turgidus under different physicochemical conditions. Parasitol Res 90:435–437CrossRefPubMedGoogle Scholar
  23. LaRue GR (1957) The classification of digenetic Trematoda: a review and a new system. Exp Parasitol 6:306–349CrossRefGoogle Scholar
  24. Li SY, Chung YB, Chung BS, Choi MH, Yu JR, Hong ST (2004) The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment. Parasitol Res 93:562–566CrossRefGoogle Scholar
  25. Negm-Eldin M, Davies RW (2002) Morphology and life cycle of Apatemon hypseleotris species novum from Australia including metacercariae viability and excystment. Dtsch Tierarztl Wochenschr 109:306–314PubMedGoogle Scholar
  26. Ooi HK, Chen CI, Oku Y (2002) Excystation of Haplorchis taichui metacercariae could be elicited by change in pH. Jpn J Vet Res 50:3–7PubMedGoogle Scholar
  27. Peoples RC, Fried B (2008) The effects of various chemical and physical factors on encystment and excystment of Zygocotyle lunata. Parasitol Res 104:899–904CrossRefGoogle Scholar
  28. Reddy A, Ponder EL, Fried B (2004) Effects of copper sulfate toxicity on cercariae and metacercariae of Echinostoma caproni and Echinostoma trivolvis and on the survival of Biomphalaria glabrata snails. J Parasitol 90:1332–1337CrossRefPubMedGoogle Scholar
  29. Rossi ML, Balfour CD, Fried B (2001) Excystation and infectivity of Echinostoma caproni metacercariae after prolonged storage. Comp Parasitol 68:126–129Google Scholar
  30. Saxton TM, Fried B (2008) Excystation of the metacercarial cysts of Echinostoma caproni in a frozen and thawed trypsin–bile salts–cysteine medium. Parasitol Res 102:809–810CrossRefPubMedGoogle Scholar
  31. Saxton TM, Fried B, Peoples RC (2008) Excystation of the encysted metacercariae of Echinostoma trivolvis and Echinostoma caproni in a trypsin–bile salts–cysteine medium and Morphometric analysis of the excysted larvae. J Parasitol 94:669–671PubMedGoogle Scholar
  32. Schmidt KA, Fried B (1996) Emergence of cercariae of Echinostoma trivolvis from Helisoma trivolvis under different conditions. J Parasitol 82:674–676CrossRefPubMedGoogle Scholar
  33. Smoluk JL, Fried B (1994) Light microscopic observations of Echinostoma trivolvis (Trematoda) metacercariae during in vitro excystation. Parasitol Res 80:435–438CrossRefPubMedGoogle Scholar
  34. Ursone R, Fried B (1995) Light and scanning electron microscopy of Echinostoma caproni (Trematoda) during maturation in ICR mice. Parasitol Res 81:45–51CrossRefPubMedGoogle Scholar
  35. Xiao X, Wang TP, Zheng XD, Shen GJ, Tian ZG (2005) In vivo and in vitro encystment of Echinochasmus liliputanus cercariae and biological activity of the metacercariae. J Parasitol 91:492–498CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Biological SciencesSam Houston State UniversityHuntsvilleUSA
  2. 2.Department of BiologyLafayette CollegeEastonUSA

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