Parasitology Research

, Volume 105, Issue 4, pp 1085–1091 | Cite as

Comparative efficacy of Annona squamosa and Azadirachta indica extracts against Boophilus microplus Izatnagar isolate

  • Shivanand Magadum
  • D. B. Mondal
  • S. Ghosh
Original Paper


In the search of developing herbal acaricides, eight medicinal plants were screened for their efficacy against Boophilus microplus, the widely distributed tick species in India. Of the seven extracts screened, the extracts prepared from the Annona squamosa seed showed very high level of efficacy (70.8%) after 24 h of treatment. The effect of treatment on oviposition of the survived ticks was also assessed, and a significant reduction (P < 0.05) in the reproductive index was noted in comparison to control. When efficacy of the in vitro optimized concentration of A. squamosa was compared with previously tested extract of Azadirachta indica in in vivo model, it was observed that the extracts prepared from A. indica is more efficacious than the extracts of A. squamosa. A comparable efficacy against B. microplus fed on animals treated with herbal extracts and commonly used synthetic acaricide was noted. The possibility of using the herbal extracts in IPM format for the management of ticks is discussed.


Cypermethrin Babesia Tick Infestation Chlorpyriphos Reproductive Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Sincere thanks are due to Indian Council of Agricultural Research for providing financial support for conducting the experiment. The publication of the work is facilitated through National Agricultural Innovation Project No. NAIP/Comp-4/C2066/2007-08.


  1. Benavides OE, Hernandez MG, Castro RNA, AH RBJL (2001) Preliminary evaluation of neem (Azadirachta indica) extracts as alternative for cattle ticks Boophilus microplus. Riv Columb Entomol 27:1–8Google Scholar
  2. Bicalho KA, Ferreire F, Borges LMF, Ribeiro MFB (2001) In vitro evaluation of the effects of some acaricides on life stages of Rhipicephalus sanguineus (Acari: Ixodidae). Arq Bras Med Vet Zootec 53:548–552CrossRefGoogle Scholar
  3. Chungsamarnyart NS, Jiwajinda W, Jansawan U, Buranasilpin KP (1988) Effective plant crude-extracts on the ticks (Boophilus microplus). I. Larvicidal Action. Kasetsart J Nat Sci Suppl 22:37–41Google Scholar
  4. FAO (2004) Resistance management and integrated parasite control in ruminants. Guidelines. Animal Production and Health Division, pp 25–77Google Scholar
  5. Finney DJ (1962) Probit analysis—a statistical treatment of the sigmoid response curve. Cambridge University Press, Cambridge, pp 1–318Google Scholar
  6. Ghosh S, Azhahianambi P (2007) Laboratory rearing of Theileria annulata-free Hyalomma anatolicum anatolicum ticks. Exp Appl Acarol 43:137–146PubMedCrossRefGoogle Scholar
  7. Ghosh S, Singh NK, Das G (2005) Assessment of duration of immunity in calves immunized by glycoproteins isolated from the larvae of Hyalomma anatolicum anatolicum and Boophilus microplus. Parasitol Res 95:319–326PubMedCrossRefGoogle Scholar
  8. Ghosh S, Azhahianambi P, De la Fuente J (2006) Control of ticks of ruminants with special emphasis on livestock farming system in India—present and future possibilities for integrated control: a review. Exp Appl Acarol 40:49–66PubMedCrossRefGoogle Scholar
  9. Graf JF, Gogolewski R, Leach BN (2004) Tick control: an industry point of view. Parasitology 129:S247–S442CrossRefGoogle Scholar
  10. Hart RJ (1986) Mode of action of agent used against arthropod parasites. Chemotherapy of parasitic diseases. Plenum, New York, pp 585–601Google Scholar
  11. Kalakumar B, Kumar HSA, Kumar BA, Reddy KS (2000) Evaluation of custard seed oil and neem oil as acaricides. J Vet Parasitol 14:171–172Google Scholar
  12. Khan MH, Naithani RC, Singh (kr) S (1982) Study on the bionomics of Nosoma monostrosum (Nuttall and Warburton) (Acari: Ixodidae). Acarologia 23:119–123Google Scholar
  13. Minjauw B, Mc Leod A (2003) Tick borne diseases and poverty. The impact of tick and tick borne diseases on the livelihoods of small-scale and marginal livestock owners in India and eastern and southern Africa. Research report, DFID Animal Health Programme, Centre for Tropical Veterinary Medicine, University of Edinburgh, UKGoogle Scholar
  14. Narang C, Suganya M, Chanarang R, Surat Wadee J, Weerapol J (1988) Effective plant crude extract on tick (B. Microphilus)-Larvicidal action. Kasetstart Jl Nat Sci Suppl 22:37–41Google Scholar
  15. Nari A (1995) Stratigies for control of one host ticks and relationship with tick borne diseases in South Africa. Vet Parasitol 57:153–165PubMedCrossRefGoogle Scholar
  16. Nongluck S, Wandee G, Aim-On S, Lekcharoen K, Pongkrit T (2006) Toxicity testing of organic solvent extracts from Annona squamosa: effects on rabbit eyes and ear skin. Phytother Res 8:365–368Google Scholar
  17. Shankaram AVB, Murthy MM, Akkewar DM, Subramanyam M, Rao AN (2006) United States patent no. 6,991,818.Google Scholar
  18. Snedecor GW, Cochran WG (1968) Statistical methods. Oxford and IBH, Calcutta, India, pp 1–593Google Scholar
  19. Srivastava R, Ghosh S, Mondal DB, Azhahianambi P, Singhal PSD, Pandey NN, Swarup D (2008) Efficacy of Azadirachta indica extracts against Boophilus microplus. Parasitol Res 104:149–153PubMedCrossRefGoogle Scholar
  20. WHO (1993) Vector resistance to pesticide. Fifteenth Report of the WHO Expert Committee on Vector Biology and Control. Technical Repot Service, pp 818, 58, WHO, Geneva.Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Division of MedicineIndian Veterinary Research InstituteIzatnagarIndia
  2. 2.Entomology Laboratory, Division of Veterinary ParasitologyIndian Veterinary Research InstituteIzatnagarIndia

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