Parasitology Research

, 105:949 | Cite as

High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain)

  • M. Victoria Domínguez-Márquez
  • Remedios Guna
  • Carlos Muñoz
  • M. Teresa Gómez-Muñoz
  • Rafael Borrás
Original Paper

Abstract

In order to know the genetic diversity of Blastocystis hominis from a health district of Valencia (Spain) 51 clinical isolates from symptomatic patients,31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (RFLP) of amplicons obtained by polymerase chain reaction (PCR) of small-subunit of ribosomal DNA genes (SSU-rDNA). For this purpose, DNA was subjected to two independent PCR (RD3-RD5, F1-R1) and to three independent treatments with restrictases (AluI, HinfI and RsaI). The digested DNA was separated electrophoretically, the isolates were clustered into ribotypes (ribodemes, RD3-RD5; subgroups, F1-R1) according to their profiles and the results were translated into genetic subtypes (ST) proposed by a consensus terminology. The results show that the isolates studied are an heterogeneous population and that both PCR-RFLP SSU-rDNA protocols have a similar discriminative power, since it allowed the ribotyping of all isolates and their clustering into four demes: ribodemes 1, 3 and 3-r and 6, which include isolates belonging to subgroup III, IV, V and V-r, respectively; which were assigned to ST1 (2%), ST2 (3.9%) and ST4 (94.1%). The most common of which is a zoonotic subtype (Blastocystis ratti) which includes, according to recent studies, non-pathogenic and pathogenic variants.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • M. Victoria Domínguez-Márquez
    • 1
    • 3
  • Remedios Guna
    • 2
    • 3
  • Carlos Muñoz
    • 3
  • M. Teresa Gómez-Muñoz
    • 4
  • Rafael Borrás
    • 3
    • 5
  1. 1.Servicio de MicrobiologíaHospital Universitario de La RiberaAlziraSpain
  2. 2.Unidad de MicrobiologíaConsorcio Hospital General UniversitarioValenciaSpain
  3. 3.Departamento de Microbiología y Ecología, Facultad de Medicina y OdontologíaUniversidad de ValenciaValenciaSpain
  4. 4.Departamento de Sanidad AnimalUniversidad Cardenal Herrera-CEUMoncadaSpain
  5. 5.Servicio de MicrobiologíaHospital Clínico UniversitarioValenciaSpain

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