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Parasitology Research

, Volume 105, Issue 4, pp 967–976 | Cite as

Comparative in vitro effect of artemether and albendazole on adult Toxocara canis

  • H. A. ShalabyEmail author
  • S. Abdel-Shafy
  • K. A. Abdel-Rahman
  • A. A. Derbala
Original Paper

Abstract

Since the integrity of Toxocara canis cuticle is essential for the nutritive and protective functions, light and scanning electron microscopic studies were undertaken to assess, for the first time, whether artemether had any effect on the cuticle including lips and sensory organs following 24 and 48 h incubation in vitro. The results were compared with those observed in the worm cuticle following incubation in albendazole, as it was 100% effective against adult nematode. The body cover changes seen after in vitro administration of artemether were similar to that induced by albendazole sulfoxide, active form, (ABZ-SO). However, the earlier onset of those changes was recorded with the former. The swollen appearance of the anterior end of T. canis, including the lips, distortion of some sensory papillae, and appearance of a number of lesions on the lips, were observed during in vitro action of artemether. Cuticular disruption had also been observed in adult T canis exposed to ABZ-SO. The surface changes were more severe than those observed following incubation in artemether, with which limited loss of the cuticle occurred in the lips of nematode but was not as widespread as that seen with ABZ-SO. However, the cuticular swelling of the anterior end of T canis was more pronounced than that with ABZ-SO. In the present study, artemether presented itself as a drug that might become important in nematode chemotherapy, besides its broad spectrum of activity against various trematodes.

Keywords

Canis Artemisinin Adult Worm Albendazole Artemether 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Al-Jabr OA, Storey DM, Akrigg A, Bryden AS (1997) Prevalence of Toxocara ova in dog faeces. Vet Rec 140:211–212PubMedGoogle Scholar
  2. Alvarez LI, Mottier ML, Lanusse CE (2006) Drug transfer into target helminth parasites. Trends Parasitol 23:97–104CrossRefGoogle Scholar
  3. Aukštikalniene R, Kublickiene O, Vyšniauskas A, Žilinskiene R (2000) Histological investigations of Toxocara canis tissues and their micromorphological changes under the action of albendazole in vivo. Acta Zool Lit 10:74–84Google Scholar
  4. Bogojavlenskij JK, Bogolepova II, Opushko NB (1982) Microstructure of parasite nematodes. Science, Moscow (in Russian)Google Scholar
  5. Bricker CS, Depenbusch JW, Bennett JL, Thompson DP (1983) The relationship between tegumental disruption and muscle contraction in Schistosoma mansoni exposed to various compounds. Z Parasitenkd 69:61–71PubMedCrossRefGoogle Scholar
  6. Buchanan JF, Fairweather I, Brennan GP, Trudgett A, Hoey EM (2003) Fasciola hepatica: surface and internal tegumental changes induced by treatment in vitro with sulphoxide metabolite of albendazole (‘Valbazen’). Parasitology 126:141–153PubMedCrossRefGoogle Scholar
  7. Carson FL (1926) Histotechnology. A self Instructional Text. ASCP, Chicago, ILGoogle Scholar
  8. Despommier D (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev 16:265–272PubMedCrossRefGoogle Scholar
  9. Deutz A, Fuchs K, Auer H, Kerbl U, Aspöck H, Köfer J (2005) Toxocara infestations in Austria: a study on the risk of infections of farmers, slaughterhouse staff, hunters and veterinarians. Parasitol Res 97:390–394PubMedCrossRefGoogle Scholar
  10. Fetterer R, Rew RS, Knight R (1982) Comparative efficacy of albendazole against Fasciola hepatica in sheep and calves: relationship to serum drug metabolite levels. Vet Parasitol 11:309–316PubMedCrossRefGoogle Scholar
  11. Golenser J, Waknine JH, Krugliak M, Hunt NH, Grau GE (2006) Current perspectives on the mechanism of action of artemisinins. Int J Parasitol 36:1427–1441PubMedCrossRefGoogle Scholar
  12. Hanser E, Mehlhorn H, Hoeben D, Vlaminck K (2002) In vitro studies on the effects of flubendazole against Toxocara canis and Ascaris suum. Parasitol Res 89:63–74PubMedCrossRefGoogle Scholar
  13. Haynes RK (2006) From artemisinin to new artemisinin antimalarials: biosynthesis, extraction, old and new derivatives, stereochemistry and medicinal chemistry requirements. Curr Top Med Chem 6:509–537PubMedCrossRefGoogle Scholar
  14. Ingold K, Bigler P, Thorman W, Cavaliero T, Gottstein B, Hemphill A (1999) Efficacies of albendazole sulphoxide and albendazole sulphone against in vitro cultivated Echinococcus multilocularis metacestodes. Antimicrob Agents Chemother 43:1052–1061PubMedGoogle Scholar
  15. Keiser J, Utzinger J (2007) Artemisinins and synthetic trioxolanes in the treatment of helminth infections. Curr Opin Infect Dis 20:605–612PubMedCrossRefGoogle Scholar
  16. Keiser J, Morson G (2008) Fasciola hepatica: Tegumental alterations in adult flukes following in vitro and in vivo adminstration of artesunate and artemether. Exp Parasitol 118:228–237PubMedCrossRefGoogle Scholar
  17. Kozan E, Sevimli FK, Birdane FM, Adanir R (2008) Efficacy of eprinomectin against Toxocara canis. Parasitol Res 102:397–400PubMedCrossRefGoogle Scholar
  18. Lacey E (1990) Mode of action of benzimidazoles. Parasitol Today 6:112–115PubMedCrossRefGoogle Scholar
  19. Mackenstedt U, Schmidt S, Mehlhorn H, Stoye M, Traeder W (1993) Effects of pyrantel pamoate on adult and preadult Toxocara canis worms; an electron microscope and autoradiography study. Parasitol Res 79:567–578PubMedCrossRefGoogle Scholar
  20. Markoski MM, Trindade ES, Cabrera G, Laschuk A, Galanti N, Zaha A, Nader HB, Ferreira HB (2006) Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes: Cestoda). Parasitol Int 55:51–61PubMedCrossRefGoogle Scholar
  21. Martin RJ, Robertson AP, Björn H (1997) Target sites of anthelmintics. Parasitol 114:111–124Google Scholar
  22. Mehlhorn H, Harder A (1997) Effects of the synergistic action of febantel and pyrantel on the nematode Heterakis spumosa: a light and transmission electron microscopy study. Parasitol Res 83:419–434PubMedCrossRefGoogle Scholar
  23. Mottier ML, Alvarez LI, Pis MA, Lanusse CE (2003) Transtegumental diffusion of benzimidazole anthelmintics into Moniezia benedeni: correlation with their octanol-water partition coefficients. Exp Parasitol 103:1–7PubMedCrossRefGoogle Scholar
  24. Overgaauw PAM (1997) Prevalence of intestinal nematodes of dogs and cats in the Netherlands. Vet Quart 19:14–17Google Scholar
  25. Perez-Serrano J, Casado N, Denegri G, Rodriguez-Caabeiro F (1994) The effects of albendazole and albendazole sulphoxide combination therapy on Echinococcus granulosus in vitro. Int J Parasitol 24:219–224PubMedCrossRefGoogle Scholar
  26. Prichard R (1994) Anthelmintic resistance. Vet Parasitol 54:259–268PubMedCrossRefGoogle Scholar
  27. Roberts L, Janvory J Jr (2000) Gerald D Schmidt and Larry S. Roberts' foundations of parasitology, 6th edn. McGraw-Hill, New York, USAGoogle Scholar
  28. Sangster NC, Prichard RK, Lacey E (1985) Tubulin and benzimidazole-resistance in Trichostrongylus colubriformis (Nematoda). J Parasitol 71:645–651PubMedCrossRefGoogle Scholar
  29. Schmidt J (1998) Effects of benzimidazole anthelmintics as microtubule-active drugs on the synthesis and transport of surface glycoconjugates in Hymenolepis microstoma, Echinostoma caproni and Schistosoma mansoni. Parasitol Res 84:362–368PubMedCrossRefGoogle Scholar
  30. Shalaby HA, El Namaky AH, Kamel ROA (2009) In vitro effect of artemether and triclabendazole on adult Fasciola gigantica. Vet Parasitol 160:76–82.Google Scholar
  31. Skrjabin KI, Shikhobalova NP, Mozgovoi AA (1991) Oxyurata and Ascaridata. In: Skrjabin KI (ed) Key to parasitic nematoda, vol 2. Brill, LeidenGoogle Scholar
  32. Stettler M, Siles-Lucas M, Sarciron E, Lawton P, Gottstein P, Hemphill A (2001) Echinococcus multilocularis alkaline phosphatase as a marker for metacestode damage induced by in vitro drug treatment with albendazole sulfoxide and albendazole sulfone. Antimicrob Agents Chemother 45:2256–2262PubMedCrossRefGoogle Scholar
  33. Stitt AW, Fairweather I (1993) Fasciola hepatica: tegumental surface changes in adult and juvenile flukes following treatment in vitro with the sulphoxide metabolite of triclabendazole (Fasinex). Parasitol Res 79:529–536PubMedCrossRefGoogle Scholar
  34. Thompson D, Geary T (1995) The structure and function of helminth surfaces. In: Marr JJ, Muller M (eds) Biochemistry and molecular biology of parasites. Academic, London, UK, pp 203–232CrossRefGoogle Scholar
  35. Urrea-Paris MA, Moreno MJ, Casado N, Rodriguez-Caabeiro F (2000) In vitro effect of praziquantel and albendazole combination therapy on the larval stage of Echinococcus granulosus. Parasitol Res 86:957–964PubMedCrossRefGoogle Scholar
  36. Utzinger J, Xiao SH, Tanner M, Keiser J (2007) Artemisinins for schistosomiasis and beyond. Curr Opin Investig Drugs 8:105–116PubMedGoogle Scholar
  37. White NJ (1996) The treatment of malaria. N Engl J Med 335:800–806PubMedCrossRefGoogle Scholar
  38. Woodrow CJ, Haynes RK, Krishna S (2005) Artemisinins. Postgrad Med J 81:71–78PubMedCrossRefGoogle Scholar
  39. Xiao SH, Guo J, Chollet J, Wu J, Tanner M, Utzinger J (2003) Effect of artemether on Schistosoma japonicum: dose-efficacy relationship, and changes in worm morphology and histopathology. Chinese J Parasitol Parasit Dis 22:148–153Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • H. A. Shalaby
    • 1
    Email author
  • S. Abdel-Shafy
    • 1
  • K. A. Abdel-Rahman
    • 1
  • A. A. Derbala
    • 1
  1. 1.Department of Parasitology and Animal DiseasesNational Research CenterGizaEgypt

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