Advertisement

Parasitology Research

, 105:913 | Cite as

Resistance of different fungal structures of Duddingtonia flagrans to the digestive process and predatory ability on larvae of Haemonchus contortus and Strongyloides papillosus in goat feces

  • Artur K. Campos
  • Jackson V. Araújo
  • Marcos P. Guimarães
  • Anderson S. Dias
Original Paper

Abstract

The dynamics of the passage of conidia, chlamydospores, and mycelia of the fungus Duddingtonia flagrans through the digestive tracts of goats was evaluated. Four groups with five goats each were formed. In the group conidia, each animal received 1 × 106 D. flagrans conidia per kilogram of live weight. In the group chlamydospore, each animal received 1 × 106 chlamydospores per kilogram of live weight. In the group mycelia, each animal received 1 g of mycelium mass per kilogram of live weight. In the control group, the animals received no fungal structure. Feces were obtained 3 h before and 12, 24, 30, 36, 42, 48, 60, 72, 84, and 96 h after the inoculation. The feces were placed in Petri dishes containing water-agar. The Petri dishes were examined to detect the fungus and trapped nematodes. A second trial evaluated the effect of the fungal structures on the number of gastrointestinal larvae of Haemonchus contortus and Strongyloides papillosus harvested from the fecal cultures of the goats. The feces were obtained from the goats in the 12–24, 24–30, 30–36, 42–48, 60–72, 72–84, and 84–96 intervals after the inoculation. D. flagrans survived the digestive process of the goats and maintained its predatory activity, being observed from 12 to 96 h before inoculation in the animals that received chlamydospores and conidia.

Keywords

Larval Development Infective Larva Predatory Activity Fungal Structure Nematophagous Fungus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to CNPq and Fapemig for financial support.

References

  1. Alves PH, Araújo JV, Guimarães MP, Assis RCL, Sarti P, Campos AK (2003) Aplicação de formulação do fungo predador de nematóides Monacrosporium thaumasium (Drechsler, 1937) no controle de nematóides de bovinos. Arq Bras Med Vet Zootec 55:568–573Google Scholar
  2. Araújo JV, Guimarães MP, Campos AK, Sá NC, Sarti P, Assis RCL (2004a) Control of bovine gastrointestinal nematodes parasites using pellets of the nematode-trapping fungus Monacrosporium thaumasium. Cienc Rural 34:457–463Google Scholar
  3. Araújo JV, Mota MA, Campos AK (2004b) Controle biológico de helmintos parasitos de animais por fungos nematófagos. Rev Bras Parasitol Vet 13(1):165–171Google Scholar
  4. Assis RCL, Araújo JV, Gandra JR, Campos AK (2005) Avaliação de fungos predadores do gênero Monacrosporium sobre larvas infectantes de Haemonchus contortus de caprinos. Rev Bras Cienc Vet 12:42–45Google Scholar
  5. Barçante JMP, Barçante TA, Dias SRC, Lima WS, Negrão-Corrêa DA (2003) A method to obtain axenic Angiostrongylus vasorum first-stage larvae from dog feces. Parasitol Res 89:89–93PubMedCrossRefGoogle Scholar
  6. Chandrawathani P, Jamnah O, Waller PJ (1998) The control of the free-living stages of Strongyloides papillosus by the nematophagous fungus, Arthrobotrys oligospora. Vet Parasitol 76:321–325PubMedCrossRefGoogle Scholar
  7. Chandrawathani P, Jamnah O, Waller PJ, Höglund J, Larsen M, Zahari WM (2002) Nematophagous fungi as a biological control agent for nematode parasites of small ruminants in Malaysia; a special emphasis on Duddingtonia flagrans. Vet Res 33:685–696PubMedCrossRefGoogle Scholar
  8. Gonzalez-Cruz ME, Mendoza-de-Gives P, Quiroz-Romero H (1998) Comparison of the ability of Arthrobotrys robusta and Monacrosporium gehyropagum on infective larvae of Strongyloides papillosus. J Helminthol 72:209–213PubMedCrossRefGoogle Scholar
  9. Gordon HM, Whitlock HV (1939) A new technique for counting nematode eggs in sheep faeces. J Counc Sci Ind Res 12:50–52Google Scholar
  10. Gronvold J, Henriksen SA, Larsen M, Nansen P, Wolstrup J (1996) Aspects of biological control—with special reference to arthropods, protozoans and helminths of domesticated animals. Vet Parasitol 64:47–64PubMedCrossRefGoogle Scholar
  11. Larsen M (1999) Biological control of helminths. Int J Parasitol 29:139–146PubMedCrossRefGoogle Scholar
  12. Larsen M (2000) Prospects for controlling animal parasitic nematodes by predacious microfungi. Parasitology 120:121–131CrossRefGoogle Scholar
  13. Larsen M, Faedo M, Waller PJ (1998) The potential of nematophagous fungi to control the free living stages of nematode parasites of sheep: studies with Duddingtonia flagrans. Vet Parasitol 76:121–128PubMedCrossRefGoogle Scholar
  14. Llerandi-Juárez RD, Mendoza-de-Gives P (1998) Resistance of chlamydospores of nematophagous fungi to the digestive processes of sheep in Mexico. J Helminthol 72:155–158CrossRefGoogle Scholar
  15. Mendoza-de-Gives P, Zavaleta-Mejia E, Herrera-Rodrigues D, Quiroz-Romero H (1994) In vitro trapping capability of Arthrobotrys spp on infective larvae of Haemonchus contortus and Nacobus aberrans. J Helminthol 68:223–229CrossRefGoogle Scholar
  16. Mendoza-de-Gives P, Flores-Crespo J, Herrera-Rodrigues D, Vasquez-Prats V, Liebano Hernandez E, Ontiveros-Fernadez GE (1998) Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. J Helminthol 72:343–347PubMedCrossRefGoogle Scholar
  17. Ojeda-Robertos NF, Mendoza-de-Gives P, Torres-Acosta JFJ, Rodríguez-Vivas RI, Aguillar-Caballero AJ (2005) Evaluating the effectiveness of a Mexican strain of Duddingtonia flagrans as a biological control agent against gastrointestinal nematodes in goat faeces. J Helminthol 79:151–157PubMedCrossRefGoogle Scholar
  18. Paraud C, Pors I, Chartier C (2004) Activity of Duddingtonia flagrans on Trichostrongylus colubriformis larvae in goat feces and interaction with a benzimidazole treatment. Small Rumin Res 55:199–209CrossRefGoogle Scholar
  19. Pienaar JG, Basson PA, Plessis JLD, Collins HM, Naude TW, Boyazoglu PA, Boomker J, Reyers F, Pienaar WL (1999) Experimental studies with Strongyloides papillosus in goats. Onderstepoort J Vet Res 66:191–235PubMedGoogle Scholar
  20. Ribeiro SDA (1997) Caprinocultura: Criação racional de caprinos. Nobel, São PauloGoogle Scholar
  21. Taira N, Nakamura Y, Tsuji N, Kubo M, Ura S (1992) Sudden death of calves by experimental infection with Strongyloides papillosus. I. Parasitological observations. Vet Parasitol 42:247–256Google Scholar
  22. Terril TH, Larsen M, Samples O, Husted S, Miller JE, Kaplan RM, Gelaye S (2004) Capability of the nematode-trapping fungus Duddingtonia flagrans to reduce infective larvae of gastrointestinal nematodes in goat faeces in the southeastern United States: dose titration and dose time interval studies. Vet Parasitol 120:285–296CrossRefGoogle Scholar
  23. Urquhart GM, Armour J, Duncan JL (1996) Veterinary parasitology. Blackwell Science, London, UK, p 307Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Artur K. Campos
    • 1
    • 3
  • Jackson V. Araújo
    • 2
  • Marcos P. Guimarães
    • 1
  • Anderson S. Dias
    • 4
  1. 1.Departamento de Parasitologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Departamento de VeterináriaUniversidade Federal de ViçosaViçosaBrazil
  3. 3.UNIVIÇOSAViçosaBrazil
  4. 4.IESES/FACASTELOCasteloBrazil

Personalised recommendations