Parasitology Research

, 105:809 | Cite as

Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA

  • Mao Peng
  • Mie Ichinomiya
  • Maiko Ohtori
  • Madoka Ichikawa
  • Toshiyuki Shibahara
  • Tadashi Itagaki
Original Paper


Parthenogenic Fasciola forms as well as bisexual Fasciola hepatica and Fasciola gigantica in mainland China have been identified on the basis of their spermatogenesis and genotypes in nuclear ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase I (NDI). The Chinese aspermic Fasciola would include forms originating in interspecific hybrids between F. hepatica and F. gigantica, since they showed the genotype of ITS1-Fh/Fg that had mixed sequences of the two Fasciola species or heterogeneous genotypes in ITS1 and NDI. Additionally, there were Chinese aspermic flukes in which the sequences of ITS1 and NDI genotypes completely coincided with those in aspermic forms from Japan, Korea, and Vietnam, suggesting that the aspermic forms from these four countries are offspring with a common provenance. The Fh-C4 haplotype in NDI was detected in both aspermic specimens and F. hepatica, indicating that aspermic forms showing the haplotype might come into existence in China. The ratio of body length and width in aspermic Fasciola specimens showed intermediate values between those of F. hepatica and F. gigantica.


Body Length Polymerase Chain Reaction Amplicon Zebu Cattle Heterogeneous Genotype Taurine Cattle 
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This study was supported in part by a Grant-in-Aid for Scientific Research (B) (no. 18405035) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Mao Peng
    • 1
    • 2
  • Mie Ichinomiya
    • 1
  • Maiko Ohtori
    • 1
  • Madoka Ichikawa
    • 1
  • Toshiyuki Shibahara
    • 3
  • Tadashi Itagaki
    • 1
  1. 1.Laboratory of Veterinary Parasitology, Faculty of AgricultureIwate UniversityMoriokaJapan
  2. 2.Academy of Animal and Veterinary MedicineQinghai UniversityXiningChina
  3. 3.Laboratory of Comparative Animal Science, Department of Animal Pharmaceutical Science, Faculty of PharmacyChiba Institute of ScienceChoshiJapan

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