Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products
- 322 Downloads
Clonorchiasis is associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Although this is likely caused by adult Clonorchis sinensis and its excretory–secretory products (ESP), the precise molecular mechanisms remain obscure. To evaluate the effect of C. sinensis infection on differential gene expression in host hepatocytes, we examined the kinetics of changes in gene expression in the human cholangiocarcinoma cell line HuCCT1 treated with ESP at different times. Using complementary DNA microarrays containing 23,920 human genes of known function, we initially identified 435 genes altered by ESP treatment. Of these, 31 were up-regulated and 35 were down-regulated more than twofold in a time-dependent manner following ESP treatment. Clustering of these genes by function revealed that several were involved in the cell cycle, oncogenesis, protein modification, immunity, signal transduction, cell structure, and developmental processes. These findings should provide a fundamental basis for further analysis of the molecular pathways and mechanisms involved in C. sinensis infection of host cells.
KeywordsClonorchiasis Opisthorchis Viverrini HuCCT1 Cell Cold Physiological Saline Human Cholangiocarcinoma Cell Line
The authors thank Macrogen, Inc. (Seoul, Korea) for technical assistance with cDNA microarrays. This work was supported by a grant (NIH-4847-302-260-00) from the National Institute of Health, Ministry of Health and Welfare, Korea.
- Kelner MJ, Bagnell RD, Montoya MA, Estes LA, Forsberg L, Morgenstern R (2000) Structural organization of the microsomal glutathione S-transferase gene (MGST1) on chromosome 12p13.1-13.2. Identification of the correct promoter region and demonstration of transcriptional regulation in response to oxidative stress. J Biol Chem 275:13000–13006PubMedCrossRefGoogle Scholar
- Modestou M, Puig-Antich V, Korgaonkar C, Eapen A, Quelle DE (2001) The alternative reading frame tumor suppressor inhibits growth through p21-dependent and p21-independent pathways. Cancer Res 62:3145–3150Google Scholar
- Orian-Rousseau V, Aberdam D, Rousselle P, Messent A, Gavrilovic J, Meneguzzi G, Kedinger M, Simon-Assmann P (1998) Human colonic cancer cells synthesize and adhere to laminin-5. Their adhesion to laminin-5 involves multiple receptors among which is integrin alpha2beta1. J Cell Sci 111:1993–2004PubMedGoogle Scholar