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Parasitology Research

, Volume 104, Issue 5, pp 1035–1046 | Cite as

Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products

  • Jhang Ho Pak
  • Dong-Wook Kim
  • Ju Hyun Moon
  • Joo-Hyun Nam
  • Jong-Hyeok Kim
  • Jung Won Ju
  • Tong-Soo Kim
  • Sang-Beom Seo
Original Paper

Abstract

Clonorchiasis is associated with bile duct malignancy and the subsequent development of cholangiocarcinoma. Although this is likely caused by adult Clonorchis sinensis and its excretory–secretory products (ESP), the precise molecular mechanisms remain obscure. To evaluate the effect of C. sinensis infection on differential gene expression in host hepatocytes, we examined the kinetics of changes in gene expression in the human cholangiocarcinoma cell line HuCCT1 treated with ESP at different times. Using complementary DNA microarrays containing 23,920 human genes of known function, we initially identified 435 genes altered by ESP treatment. Of these, 31 were up-regulated and 35 were down-regulated more than twofold in a time-dependent manner following ESP treatment. Clustering of these genes by function revealed that several were involved in the cell cycle, oncogenesis, protein modification, immunity, signal transduction, cell structure, and developmental processes. These findings should provide a fundamental basis for further analysis of the molecular pathways and mechanisms involved in C. sinensis infection of host cells.

Keywords

Clonorchiasis Opisthorchis Viverrini HuCCT1 Cell Cold Physiological Saline Human Cholangiocarcinoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

The authors thank Macrogen, Inc. (Seoul, Korea) for technical assistance with cDNA microarrays. This work was supported by a grant (NIH-4847-302-260-00) from the National Institute of Health, Ministry of Health and Welfare, Korea.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jhang Ho Pak
    • 1
  • Dong-Wook Kim
    • 2
  • Ju Hyun Moon
    • 1
  • Joo-Hyun Nam
    • 3
  • Jong-Hyeok Kim
    • 3
  • Jung Won Ju
    • 4
  • Tong-Soo Kim
    • 5
  • Sang-Beom Seo
    • 2
  1. 1.Asan Institute for Life SciencesUniversity of Ulsan College of Medicine, Asan Medical CenterSeoulKorea
  2. 2.Department of Life Sciences, College of Natural SciencesChung-Ang UniversitySeoulKorea
  3. 3.Department of Obstetrics and GynecologyUniversity of Ulsan College of Medicine, Asan Medical CenterSeoulKorea
  4. 4.Division of Malaria and Parasitic DiseasesNational Institute of HealthSeoulKorea
  5. 5.Department of Parasitology, College of MedicineInha UniversityIncheonKorea

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