Parasitology Research

, 104:927

A proteome reference map of Trichomonas vaginalis

  • Kuo-Yang Huang
  • Kuen-Yi Chien
  • Yin-Chun Lin
  • Wei-Min Hsu
  • I-Kai Fong
  • Po-Jung Huang
  • Yuan-Ming Yueh
  • Richie Ruei-Chi Gan
  • Petrus Tang
Original Paper

DOI: 10.1007/s00436-008-1274-z

Cite this article as:
Huang, KY., Chien, KY., Lin, YC. et al. Parasitol Res (2009) 104: 927. doi:10.1007/s00436-008-1274-z

Abstract

Trichomoniasis caused by Trichomonas vaginalis is the most common sexual transmitted infection in the world. The 170-MB genome of this protozoan contains 60,000 genes, the largest number of genes ever identified in protozoan. High-throughput expression sequenced tag analysis showed that at least 4,000 genes were expressed in the trophozoite stage. In the present study, we use two-dimensional electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis to profile, identify, and characterize proteins expressed in the trophozoite stage of T. vaginalis. A total of 247 spots representing 164 different proteins were identified. The identified proteins with known sequence or motif/domain homologies were further classified into groups according to their biological functions. Among them, proteins related to carbohydrate metabolism represented the most abundant category in the T. vaginalis proteome. This study presented the most extensive proteomic analysis of T. vaginalis to date and provided a reference proteome database for future comparative proteomic studies.

Supplementary material

436_2008_1274_MOESM1_ESM.pdf (37 kb)
Table S1T. vaginalis proteins separated in the range of pH 4–7 and identified by MALDI-TOF peptide mass fingerprinting (PDF 36.9 KB).
436_2008_1274_MOESM2_ESM.pdf (26 kb)
Table S2. T. vaginalis proteins separated in the range of pH 6–11 and identified by MALDI-TOF peptide mass fingerprinting (PDF 26.3 KB).

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Kuo-Yang Huang
    • 1
  • Kuen-Yi Chien
    • 2
  • Yin-Chun Lin
    • 1
  • Wei-Min Hsu
    • 1
  • I-Kai Fong
    • 1
  • Po-Jung Huang
    • 3
  • Yuan-Ming Yueh
    • 3
  • Richie Ruei-Chi Gan
    • 3
  • Petrus Tang
    • 1
    • 3
  1. 1.Molecular Regulation and Bioinformatics Laboratory, Department of ParasitologyChang Gung UniversityTaoyuanTaiwan
  2. 2.Proteomics CenterChang Gung UniversityTaoyuanTaiwan
  3. 3.Bioinformatics CenterChang Gung UniversityTaoyuanTaiwan

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