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Parasitology Research

, 104:533 | Cite as

Effect of single-dose oral artemether and tribendimidine on the tegument of adult Clonorchis sinensis in rats

  • Shu-Hua Xiao
  • Jennifer Keiser
  • Jian Xue
  • Marcel Tanner
  • Gianni Morson
  • Jürg Utzinger
Original Paper

Abstract

The tegument of trematodes plays a key role in nutrient absorption, exerts secretory functions, protects the parasite against the immune system of the host, and is a target for anti-trematocidal drugs. We performed a temporal examination of tegumental changes following artemether and tribendimidine administration on adult Clonorchis sinensis in rats using scanning electron microscopy. Rats infected with C. sinensis for 6 weeks were treated orally with a single dose of artemether (150 mg/kg) or tribendimidine (300 mg/kg). Worms were collected between 8 h and 7 days (artemether) and between 4 h and 2 days post-treatment (tribendimidine). Worms recovered from untreated rats served as controls. Eight hours after artemether administration, the tegument of C. sinensis was extensively disrupted, including severe swelling, fusion and vacuolization, and the suckers were damaged. Four hours after administration of tribendimidine, C. sinensis worms showed extensive tegumental alterations, characterized by massive sloughing, and the suckers were damaged. Interestingly, the severity of tegumental changes did not progress further with time. Our results show that both artemether and tribendimidine rapidly disrupt the tegument and damage the suckers of adult C. sinensis. The subtle differences in tegumental changes induced by artemether and tribendimidine might indicate different mechanisms of action of these drugs against C. sinensis.

Keywords

Praziquantel Ventral Sucker Artesunate Oral Sucker Artemether 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This investigation received financial support from the National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Shanghai, China) and Swiss Tropical Institute (Basel, Switzerland). J. Keiser (project no. PPOOA-114941) and J. Utzinger (project no. PPOOB-102883, PPOOB-119129) are grateful to the Swiss National Science Foundation for financial support.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Shu-Hua Xiao
    • 1
  • Jennifer Keiser
    • 2
  • Jian Xue
    • 1
  • Marcel Tanner
    • 3
  • Gianni Morson
    • 4
  • Jürg Utzinger
    • 3
  1. 1.National Institute of Parasitic DiseasesChinese Center for Disease Control and PreventionShanghaiPeople’s Republic of China
  2. 2.Department of Medical Parasitology and Infection BiologySwiss Tropical InstituteBaselSwitzerland
  3. 3.Department of Public Health and EpidemiologySwiss Tropical InstituteBaselSwitzerland
  4. 4.Center for Microscopy, BiozentrumUniversity of BaselBaselSwitzerland

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