Parasitology Research

, 103:1347

Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

  • Chadaporn Nuchjangreed
  • Kajohn Boonrod
  • Jerry Ongerth
  • Panagiotis Karanis
Original Paper

DOI: 10.1007/s00436-008-1139-5

Cite this article as:
Nuchjangreed, C., Boonrod, K., Ongerth, J. et al. Parasitol Res (2008) 103: 1347. doi:10.1007/s00436-008-1139-5

Abstract

This study was performed to determine the prevalence and genotypes of Cryptosporidium species among HIV patients and cattle in Thailand. Stool specimens were collected from 46 HIV patients from Prabat Nampu Temple, Lop Buri Province in central Thailand. Two hundred fecal samples from dairy cattle were collected from seven farms in Chon Buri Province, the eastern part of Thailand. Each sample was concentrated by Sheather’s sucrose flotation technique and stained by acid fast stain (AFS) for the identification of oocysts by microscopy. All HIV stool samples and 83 fecal specimens from cattle were further tested using nested polymerase chain reaction (PCR) targeting the 18S SSUrRNA gene to characterize the detected species. In HIV patient samples, the detection rate was 28.7% by AFS and 4.35% by nested PCR. In cattle samples, the detection rate was 13% by AFS and 9.63% by nested PCR. After DNA sequencing results, we identified the genotypes of the Cryptosporidium from seven of the PCR positive samples. All were found to be C. parvum. The findings presented here represent the first genetic identification of Cryptosporidium species in cattle in Thailand.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Chadaporn Nuchjangreed
    • 1
  • Kajohn Boonrod
    • 2
  • Jerry Ongerth
    • 3
  • Panagiotis Karanis
    • 4
    • 5
  1. 1.Medical Science Department, Faculty of ScienceBurapha UniversityBangsaenThailand
  2. 2.AIPlanta AgroScience GmbHNeustadt/WeinstrasseGermany
  3. 3.Civil Mining & Environmental EngineeringThe University of WollongongWollongongAustralia
  4. 4.Medical and Molecular Parasitology Laboratory, Medical School, Center of Anatomy, Institute IIUniversity of CologneCologneGermany
  5. 5.National Research Center for Protozoan DiseasesObihiro University of Agriculture and Veterinary MedicineObihiroJapan

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