Castalagin from Anogeissus leiocarpus mediates the killing of Leishmania in vitro
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Stem barks of Anogeissus leiocarpus and Terminalia avicennoides widely used in Africa for treatment of some parasitic diseases were collected and made into methanolic extracts. The extracts were tested on four strains of promastigote forms of Leishmania in vitro. Solvent fractionation in aqueous, butanolic, and ethyl acetate layer indicated butanol and aqueous fractions to have a superior leishmanicidal activity. Chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolyzable tannins and some related compounds—with castalagin as the major compound. The observed activity ranged from 62.5 to ≥150, 112.5 to ≥500, and 55 to >150 μg/ml for the crude methanolic extract, different solvent fractions, and the isolated compounds, respectively, on the four different Leishmania strains.
KeywordsLeishmaniasis Hydrolyzable Tannin Crude Methanolic Extract Antileishmanial Activity Punicalagin
This work was supported by the Japanese Society for the Promotion of Science (JSPS). MNS was a JSPS fellow and wishes to thank JSPS for the opportunity given to conduct this research.
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