Parasitology Research

, Volume 103, Issue 6, pp 1317–1322 | Cite as

Crossing experiment of Anopheles maculatus form K and Anopheles willmori (James) (Diptera: Culicidae)

  • Pradya Somboon
  • Damrongpan Thongwat
  • Katy Morgan
  • Catherine Walton
Original Paper
First Online:
Received:
Accepted:

Abstract

We recently reported crossing experiments between Anopheles maculatus form K and five members of the Maculatus group to support the specific status of form K. In the present study, we performed further crosses between form K and a sixth species of the Maculatus group, Anopheles willmori (James). Low viability was observed in hybrid males and females. All hybrid males were sterile with atrophied testes, or partially sterile with abnormal spermatozoa. The hybrid females showed normal ovaries. The ovarian nurse cell polytene chromosomes of the F1 hybrid females displayed complete asynapsis. Backcrossing showed low viability. All males were sterile with atrophied testes or partially sterile with abnormal spermatozoa, and the females showed varying degrees of atrophied ovaries. The results provide clear evidence that form K is not conspecific to A. willmori supporting previous studies that form K represents another species of the Maculatus group.

Keywords

Polytene Chromosome Hybrid Male Hybrid Female Atrophied Testis Ovarian Nurse Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This study was supported by The Royal Golden Jubilee Ph.D. Program, The Thailand Research Fund, and in part by the Faculty of Medicine Research Fund, Chiang Mai University, Chiang Mai, Thailand. We would like to thank Rampa Rattanarithikul for her suggestions and Jetsumon Sattabongkot for donating specimens of A. notanandai. We also thank The Vector-Borne Disease Control Programme, Gelephu, Bhutan, especially Mr. Nima Wangdi Gyeltshen for arranging the collection of mosquitoes in Bhutan.

References

  1. Baimai V, Kijchalao U, Rattanarithikul R, Green CA (1993) Metaphase karyotypes of Anopheles of Thailand and Southeast Asia: II. Maculatus group, Neocellia series, subgenus Cellia. Mosq Syst 25:116–123Google Scholar
  2. Chabpunnarat S (1988) Cytogenetic study of the Anopheles maculatus complex. MSc thesis. Mahidol University, BangkokGoogle Scholar
  3. Chen B, Butlin RK, Pedro PM, Wang XZ, Harbach RE (2006) Molecular variation, systematics and distribution of the Anopheles fluviatilis complex in southern Asia. Med Vet Entomol 20:33–43PubMedCrossRefGoogle Scholar
  4. Christophers SR (1933) Fauna of British India, including Ceylon and Burma. Diptera. Family Culicidae. Tribe Anophelini, vol. IV. Taylor and Francis, LondonGoogle Scholar
  5. Harbach RE (2004) The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships. Bull Entomol Res 94:537–553PubMedCrossRefGoogle Scholar
  6. Harbach RE, Garros C, Manh ND, Manguin S (2007) Formal taxonomy of species C of the Anopheles minimus sibling species complex (Diptera: Culicidae). Zootaxa 1654:41–54Google Scholar
  7. Ma Y, Li S, Xu J (2006) Molecular identification and phylogeny of the Maculatus group of Anopheles mosquitoes (Diptera: Culicidae) based on nuclear and mitochondrial DNA sequences. Acta Trop 99:272–280PubMedCrossRefGoogle Scholar
  8. Ow Yang CK, Sta Maria FL, Wharton RW (1963) Maintenance of a laboratory colony Anopheles maculatus Theobald by artificial mating. Mosq News 23:34–35Google Scholar
  9. Paskewitz SM, Collins FH (1990) Use of the polymerase chain reaction to identify mosquito species of the Anopheles gambiae complex. Med Vet Entomol 4:367–373PubMedCrossRefGoogle Scholar
  10. Rattanarithikul R, Green CA (1986) Formal recognition of the species of the Anopheles maculatus group (Diptera: Culicidae) occurring in Thailand, including the descriptions of two new species and a preliminary key to females. Mosq Syst 18:246–278Google Scholar
  11. Rattanarithikul R, Harbach RE (1990) Anopheles maculatus (Diptera: Culicidae) from the type locality of Hong Kong and two new species of the Maculatus complex from the Philippines. Mosq Syst 22:160–183Google Scholar
  12. Rattanarithikul R, Harrison BA, Harbach RE, Panthusiri P, Coleman RE (2006) Illustrated keys to the mosquitoes of Thailand. IV. Anopheles. Southeast Asian J Trop Med Public Health 37(suppl 2):1–128Google Scholar
  13. Reid JA (1968) Anopheline mosquitoes of Malaya and Borneo. Stud Inst Med Res Malaysia 31:1–520Google Scholar
  14. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New YorkGoogle Scholar
  15. Singh OP, Chandra D, Nanda N, Sharma SK, Htun PT, Adak T, Subbarao SK, Dash AP (2006) On the conspecificity of Anopheles fluviatilis species S with Anopheles minimus species C. J Biosci 31:671–677PubMedCrossRefGoogle Scholar
  16. Subbarao SK (1998) Anopheline species complexes in South-East Asia. WHO, Technical Publication, SEARO No:18Google Scholar
  17. Thongwat D, Morgan K, O’ Loughlin SM, Walton C, Choochote W, Somboon P (2008) Crossing experiments supporting the specific status of Anopheles maculatus chromosomal form K. J Am Mosq Control Assoc 24:194–202PubMedCrossRefGoogle Scholar
  18. Walton C, Somboon P, O’ Loughlin SM, Zhang S, Harbach RE, Linton YM, Chen B, Nolan K, Duong S, Fong MY, Vythilingum I, Mohammed ZD, Trung HD, Butlin RK (2007) Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA. Infect Genet Evol 7:93–102PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Pradya Somboon
    • 1
  • Damrongpan Thongwat
    • 1
  • Katy Morgan
    • 2
  • Catherine Walton
    • 2
  1. 1.Department of Parasitology, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  2. 2.Faculty of Life SciencesUniversity of ManchesterManchesterUK

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