Identification of genotypes of Giardia intestinalis of human isolates in Egypt
In Egypt, the genotyping study of Giardia intestinalis in human is limited. To determine the prevalence of G. intestinalis, faecal samples were collected from Egypt. Samples were concentrated using density gradient centrifugation. The samples were subjected to PCR and DNA sequence analysis for TPI gene. Prevalence of Giardia infection was 34.6% of 52 examined. DNA sequence showed that the Assemblage B was the most prevalent (80%) genotype, the 15% of the positive samples belonged to Assemblage E, and the 5% of them belonged to Assemblage A. Certainly, both genotypes A and B are highly common in human worldwide. However, up to now, Assemblage E had not been known to be infectious for humans. Therefore, this is the first time that Assemblage E is reported in human. However, further analyses of a second locus are required to confirm this result. The extent to which Giardia-infected cattle in Egypt might pose a risk of human infection is unknown.
- Abreu-Acosta N, Lorenzo-Morales J, Leal-Guio Y, Coronado-Álvarez N, Foronda P, Alcoba-Florez J, Izquierdo F, Batista-Díaz N, Del Águila C, Valladares B (2005) Enterocytozoon bienusi (Microsporidia) in clinical samples from immunocompetent individuals in Tenerife, Canary Islands, Spain. Trans R Soc Trop Med Hyg 99:848–855PubMedCrossRefGoogle Scholar
- Ministry of Health and Population (2002) Ministry of Health and Population, 2002. Official Report of Fever Hospitals Administration. Ministry of Health and Population, CairoGoogle Scholar
- Peeters J, Villacorta I (1995) Cryptosporidium. Part II. In: Braun JR, Shiley M, Coudert P (eds) Biotechnology—Guidelines on techniques in coccidiosis research Eckert. European Commission, Directorate-General XII, Science, Research and Development, Agriculture Biotechnology, Luxembourg p. 202–240Google Scholar
- Yoder JS, Beach MJ (2007) Giardiasis surveillance—United States, 2003–2005. Surveill Summ CDC 56:11–18Google Scholar
- Zaki AM, DuPont HL, el Alamy MA, Arafat RR, Amin K, Awad MM, Bassiouni L, Imam IZ, el Malih GS, el Marsafie A, Mohieldin MS, Naguib T, Rakha MA, Sidaros M, Wasef N, Wright CE, Wyatt RG (1986) The detection of enteropathogens in acute diarrhea in a family cohort population in rural Egypt. Am J Trop Med Hyg 35:1013–1022PubMedGoogle Scholar