Parasitology Research

, Volume 103, Issue 5, pp 1177–1181 | Cite as

Identification of genotypes of Giardia intestinalis of human isolates in Egypt

  • P. Foronda
  • M. D. Bargues
  • N. Abreu-Acosta
  • M. V. Periago
  • M. A. Valero
  • B. Valladares
  • S. Mas-Coma
Original Paper

Abstract

In Egypt, the genotyping study of Giardia intestinalis in human is limited. To determine the prevalence of G. intestinalis, faecal samples were collected from Egypt. Samples were concentrated using density gradient centrifugation. The samples were subjected to PCR and DNA sequence analysis for TPI gene. Prevalence of Giardia infection was 34.6% of 52 examined. DNA sequence showed that the Assemblage B was the most prevalent (80%) genotype, the 15% of the positive samples belonged to Assemblage E, and the 5% of them belonged to Assemblage A. Certainly, both genotypes A and B are highly common in human worldwide. However, up to now, Assemblage E had not been known to be infectious for humans. Therefore, this is the first time that Assemblage E is reported in human. However, further analyses of a second locus are required to confirm this result. The extent to which Giardia-infected cattle in Egypt might pose a risk of human infection is unknown.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • P. Foronda
    • 1
  • M. D. Bargues
    • 2
  • N. Abreu-Acosta
    • 1
  • M. V. Periago
    • 2
  • M. A. Valero
    • 2
  • B. Valladares
    • 1
  • S. Mas-Coma
    • 2
  1. 1.Instituto Universitario de Enfermedades Tropicales y Salud Pública de CanariasUniversity of La LagunaTenerifeSpain
  2. 2.Department of ParasitologyUniversity of Valencia, Faculty of PharmacyValenciaSpain

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