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Parasitology Research

, 103:853 | Cite as

Development of a highly sensitive IgG-ELISA based on recombinant arginine kinase of Toxocara canis for serodiagnosis of visceral larva migrans in the murine model

  • Susiji Wickramasinghe
  • Lalani Yatawara
  • Mitsuru Nagataki
  • Misa Takamoto
  • Yoshiya Watanabe
  • R. P. V. J. Rajapakse
  • Kouji Uda
  • Tomohiko Suzuki
  • Takeshi Agatsuma
Original Paper

Abstract

Toxocariasis is a worldwide zoonotic disease caused by the ascarid nematode Toxocara canis. The most common method available for serodiagnosis of toxocariasis is an enzyme-linked immunosorbent assay (ELISA) test using Toxocara excretory-secretory antigen (TES). The present study describes the development of IgG-ELISA based on antiserum prepared against the recombinant arginine kinase of Toxocara canis. Antiserum was prepared against the purified recombinant arginine kinase (AK) using 6-week-old female Japanese white rabbits. Serum samples were collected from experimentally infected BALB/c and C57BL/6 mice at different time periods. The IgG-ELISA was performed using serum samples from mice (infected/uninfected) and TES antigen with antiserum prepared against the recombinant-AK. The optical density (OD450) was measured at 450 nm using a micro-plate ELISA reader. There were significant differences (P < 0.01) in the absorbance between infected and control serum samples. Further, we obtained 100% sensitivity for the serum samples from T. canis-infected mice. Therefore, it is suggested that the recombinant-AK based IgG-ELISA could be applied for immunodiagnosis of human toxocariasis. However, it is necessary to evaluate the specificity of this recombinant antigen with similar geohelminth infections.

Keywords

Canis ELISA Test Rabbit Antiserum Coating Antigen Arginine Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank Prof. D. Blair of James Cook University for his revision and invaluable comments.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Susiji Wickramasinghe
    • 1
  • Lalani Yatawara
    • 1
  • Mitsuru Nagataki
    • 1
    • 2
  • Misa Takamoto
    • 2
  • Yoshiya Watanabe
    • 2
  • R. P. V. J. Rajapakse
    • 3
  • Kouji Uda
    • 4
  • Tomohiko Suzuki
    • 4
  • Takeshi Agatsuma
    • 1
  1. 1.Department of Environmental Health SciencesKochi Medical SchoolOko, Nankoku CityJapan
  2. 2.Research and Development Division, Life Science CenterSophy IncorporationAgawaJapan
  3. 3.Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal ScienceUniversity of PeradeniyaPeradeniyaSri Lanka
  4. 4.Laboratory of Biochemistry, Faculty of ScienceKochi UniversityKochiJapan

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