Parasitology Research

, Volume 103, Issue 3, pp 685–689 | Cite as

A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis

  • Funda Dogruman-Al
  • Hande Dagci
  • Hisao Yoshikawa
  • Özgur Kurt
  • Mete Demirel
Original Paper

Abstract

Blastocystis hominis is one of the most common eukaryotic organisms in the intestinal tract of humans, while its pathogenic potential is still controversial. A total of 286 stool samples obtained from adult and pediatric patients with or without gastrointestinal symptoms in two hospitals in Manisa, Turkey, were cultured to detect B. hominis infection. Forty-one and 51 isolates were obtained from the adults and children, respectively, and these isolates were subjected to subtyping by polymerase chain reaction (PCR) with the known sequence-tagged site primers. The correlation between the genotype and the symptoms was evaluated. PCR subtyping indicated that subtype 3 was the most common genotype in both symptomatic and asymptomatic groups, and the second common genotype was subtypes 1 and 2 in symptomatic and asymptomatic groups, respectively. A significant correlation between subtype 2 and the asymptomatic groups was found among both in pediatric and adult patients (χ2cal = 4.38, df = 1, p = 0.044). However, there were no significant differences between the other genotypes and the symptomatic or asymptomatic groups, as well as both the age and sex of the patients. The present study suggests that subtype 2 is a non-pathogenic genotype of B. hominis.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Funda Dogruman-Al
    • 1
  • Hande Dagci
    • 2
  • Hisao Yoshikawa
    • 3
  • Özgur Kurt
    • 4
  • Mete Demirel
    • 5
  1. 1.Department of Medical MicrobiologyGazi University School of MedicineAnkaraTurkey
  2. 2.Department of ParasitologyEge University School of MedicineIzmirTurkey
  3. 3.Department of Biological Sciences, Faculty of ScienceNara Women’s UniversityNaraJapan
  4. 4.Department of Biology, Faculty of Arts and SciencesCelal Bayar UniversityManisaTurkey
  5. 5.International Pediatric HospitalMinistry of Health’s Manisa Moris SinasiManisaTurkey

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