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Parasitology Research

, 103:467 | Cite as

Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran

  • Majid Pirestani
  • Javid SadraeiEmail author
  • Abdolhossein Dalimi asl
  • Mahdi Zavvar
  • Hossein Vaeznia
Original Paper

Abstract

Cryptosporidiosis is a widespread cause of diarrheal diseases of humans, young calves, and many mammals. Although in recent years molecular investigations on Cryptosporidium have increased, no data are available on Iran in this regard. Two species of Cryptosporidium are known to infect human beings; Cryptosporidium hominis which shows anthroponotic transmission among humans and Cryptosporidium parvum which shows zoonotic transmission between animals and humans. Cryptosporidium oocysts, morphologically identified as C. parvum, were isolated from 24 human and 35 bovine cases in Shahriar (suburb of Tehran, Iran), and genotyped by means of a Nested-polymerase chain reaction/restriction fragment length polymorphism analysis of the 18s rRNA gene. The isolates from bovine samples gave zoonotic or 2 genotype (C. parvum), and DNA profiles of human isolates gave two distinct genotypes, namely an anthroponotic or 1 (C. hominis) and zoonotic genotype or 2 (C. parvum).

Keywords

Cryptosporidiosis Cryptosporidium Oocyst Zoonotic Transmission RFLP Profile Bovine Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was funded by Tarbiat Modares University. The authors wish to thank Dr. Ghafari, Dr. Sharifi, and the personnel of Imam Sadjad and Tamin Ejtemaei Hospitals and the Veterinary Organization of Tehran for their assistance.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Majid Pirestani
    • 1
  • Javid Sadraei
    • 1
    Email author
  • Abdolhossein Dalimi asl
    • 1
  • Mahdi Zavvar
    • 1
  • Hossein Vaeznia
    • 1
  1. 1.Parasitology Department, School of Medical ScienceTarbiat Modares UniversityTehranIran

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