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Parasitology Research

, Volume 103, Issue 1, pp 223–229 | Cite as

Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

  • A. Bagavan
  • A. A. RahumanEmail author
  • C. Kamaraj
  • Kannappan Geetha
Original Paper

Abstract

The acetone, chloroform, ethyl acetate, hexane and methanol leaf extracts of Acalypha indica, Achyranthes aspera, Leucas aspera, Morinda tinctoria and Ocimum sanctum were studied against the early fourth-instar larvae of Aedes aegypti L and Culex quinquefasciatus Say. The larval mortality was observed after 24 h exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in the ethyl acetate extract of A. aspera. In the present study, bioassay-guided fractionation of A. aspera led to the separation and identification of a saponin as a potential mosquito larvicidal compound, with LC50 value of 18.20 and 27.24 ppm against A. aegypti and C. quinquefasciatus, respectively. 1H NMR, 13C NMR and mass spectral data confirmed the identification of the active compound. This is the first report on the mosquito larvicidal activity of the saponin from the ethyl acetate extract of A. aspera. This study investigates the potential of crude extracts from commonly used medical herbs in India as an environmentally safe measure to control the vector of dengue and lymphatic filariasis.

Keywords

Saponin Ethyl Acetate Extract Larvicidal Activity Antifeedant Activity High Larval Mortality 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors are grateful to C. Abdul Hakeem College Management, Prof U. Peer, Principal, Dr Ahmed Najib, HOD of Zoology Department and Dr Sait Sahul Hameed, Reader in Zoology for their help and suggestion.

References

  1. Amer A, Mehlhorn H (2006a) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477PubMedCrossRefGoogle Scholar
  2. Amer A, Mehlhorn H (2006b) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472PubMedCrossRefGoogle Scholar
  3. Bagalwa M, Chifundera K (2007) Environmental impact evaluation of the stem bark extract of Maesa lanceolata used in Democratic Republic of Congo. J Ethnopharmacol 114:281–284PubMedCrossRefGoogle Scholar
  4. Banerji A, Chadha MS (1970) Insect moulting hormone from Achyranthes aspera Linn. Phytochemistry 9:1671CrossRefGoogle Scholar
  5. Batta SK, Santhakumari G (1970) The antifertility effect of Ocimum sanctum and Hibiscus rosa sinensis. Indian J Med Res 59:777–781Google Scholar
  6. Bowers WS (1992) Biorational approaches for insect control. Korean J Appl Entomol 31:289–303Google Scholar
  7. Brown AWA (1986) Insecticide resistance in mosquitoes: pragmatic review. J Am Mosq Control Assoc 2:123–140PubMedGoogle Scholar
  8. Chakraborty A, Brantner A, Mukainaka T, Nobukuni Y, Kuchide M, Konoshima T, Tokuda H, Nishino H (2002) Cancer chemopreventive activity of Achyranthes aspera leaves on Epstein–Barr virus activation and two-stage mouse skin carcinogenesis. Cancer Lett 177:1–5PubMedCrossRefGoogle Scholar
  9. Chapagain BP, Saharan V, Wiesman Z (2008) Larvicidal activity of saponins from Balanites aegyptiaca callus against Aedes aegypti mosquito. Bioresour Technol 99(5):1165–1168PubMedCrossRefGoogle Scholar
  10. Chaubal R, Pawar PV, Hebbalkar GD, Tungikar VB, Puranik VG, Deshpande VH, Deshpande NR (2005) Larvicidal activity of Acacia nilotica extracts and isolation of D-pinitol—a bioactive carbohydrate. Chem Biodivers 2(5):684–688PubMedCrossRefGoogle Scholar
  11. Chungsamarnyart N, Jiyajinda S, Jangsawan W (1991) Larvicidal effect of plant crude extracts on the tropical cattle tick (Boophilus microplus). Kasetsert J 25:80–89Google Scholar
  12. da Silva BP, de Sousa AC, Silva GM, Mendes TP, Parente JP (2002) A new bioactive steroidal saponin from Agave attenuata. Z Naturforsch [C] 57(5–6):423–428Google Scholar
  13. Daniel T, Umarani S, Sakthivadivel M (1995) Insecticidal action of Ervatamia divaricata L. and Acalypha indica L. against Culex quinquefasciatus Say. Geobios New Rep 14(2):95–98Google Scholar
  14. Deshmukh PB, Chavan SR, Renapurkar DM (1982) A study of insecticidal activity of twenty indigenous plants. Pesticides 12:7–10Google Scholar
  15. Du SH, Liu WY, Fu TJ, Xia BG, Xia CD (2002) Isolation and identification of steroidal saponins in total saponin from Dioscorea nipponica Makino. Yao Xue Xue Bao 37(4):267–270PubMedGoogle Scholar
  16. Girach RD, Khan ASA (1992) Ethnomedicinal uses of Achyranthes aspera leaves in Orissa (India). Int J Pharmacogn 30:113–115CrossRefGoogle Scholar
  17. Grainge M, Ahmed S, Mitchell WC, Hylin JW (1984) Plant species reportedly possessing pest-control properties—A database. Resource Systems Institute, East-West Center, Honolulu, HI, USA, p 240Google Scholar
  18. Gupta SS, Bhagwat AW, Ram AK (1972) Cardiac stimulant activity of the saponin of Achyranthes aspera (Linn). Indian J Med Res 60(3):462–471PubMedGoogle Scholar
  19. Han ST, Un CC (2003) Cardiac toxicity caused by Achyranthes aspera. Vet Hum Toxicol 45(4):212–213PubMedGoogle Scholar
  20. Harve G, Kamath V (2004) Larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against Aedes aegypti. Indian J Exp Biol 42(12):1216–1219PubMedGoogle Scholar
  21. Huhman DV, Sumner LW (2002) Sumner metabolic profiling of saponins in Medicago sativa and Medicago truncatula using HPLC coupled to an electrospray ion-trap mass spectrometer. Phytochemistry 59:347–360PubMedCrossRefGoogle Scholar
  22. Jang YS, Jeon JH, Lee HS (2005) Mosquito larvicidal activity of active constituent derived from Chamaecyparis obtusa leaves against 3 mosquito species. J Am Mosq Control Assoc 21:400–403PubMedCrossRefGoogle Scholar
  23. Jayasinghe UL, Jayasooriya CP, Bandara BM, Ekanayake SP, Merlini L, Assante G (2002) Antimicrobial activity of some Sri Lankan Rubiaceae and Meliaceae. Fitoterapia 73(5):424–427PubMedCrossRefGoogle Scholar
  24. Katade SR, Pawar PV, Tungikar VB, Tambe AS, Kalal KM, Wakharkar RD, Deshpande NR (2006) Larvicidal activity of bis(2-ethylhexyl) benzene-1,2-dicarboxylate from Sterculia guttata seeds against two mosquito species. Chem Biodivers 3(1):49–53PubMedCrossRefGoogle Scholar
  25. Liersch BKHR (1992) Achyranthes. In: Haensel R, Keller K, Rimpler G, Schneider G (eds) Hagers Handbuch der Pharmazeutischen Praxis, V. Springer-Verlag, Berlin7, pp 54–59Google Scholar
  26. Malik MS, Rafique M (1989) Effects of methanol extracts of Ocimum sanctum Linn. on jute semilooper. Indian J Entomol 51:84–89Google Scholar
  27. Mathivanan N, Surendiran G, Srinivasan K, Malarvizhi K (2006) Morinda pubescens JE Smith (Morinda tinctoria Roxb) fruit extract accelerates wound healing in rats. J Med Food 9(4):591–593PubMedCrossRefGoogle Scholar
  28. Murugan K, Jeyabalan D (1999) Mosquitocidal effect of certain plants extracts on Anopheles stephensi. Curr Sci 76:631–633Google Scholar
  29. Muthukrishnan J, Pushpalatha E, Kasthuribai A (1997) Biological effects of four plant extracts on Culex quinquefasciatus Say larval stages. Insect Sci Appl 17:389–394Google Scholar
  30. Omena MCD, Bento ES, De Paula JE, Sant’Ana AE (2006) Larvicidal diterpenes from Pterodon polygalaeflorus. Vector Borne Zoonotic Dis 6:216–222PubMedCrossRefGoogle Scholar
  31. Pandey V, Agrawal V, Raghavendra K, Dash AP (2007) Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Liston, Anopheles culicifacies, species C) and filaria vector (Culex quinquefasciatus Say). Parasitol Res 102(1):171–174PubMedCrossRefGoogle Scholar
  32. Pathak N, Mittal PK, Singh OP, Sagar V, Vasudevan P (2000) Larvicidal action of essential oils from plants against the vector mosquitoes Anopheles stephensi (Liston) Culex quinquefasciatus (Say) and Aedes aegypti (L). Int Pest Control 42:53Google Scholar
  33. Pelah D, Abramovich Z, Markus A, Wiesman Z (2002) The use of commercial saponin from Quillaja saponaria bark as a natural larvicidal agent against Aedes aegypti and Culex pipiens. J Ethnopharmacol 81(3):407–409PubMedCrossRefGoogle Scholar
  34. Pizarro AP, Oliveira Filho AM, Parente JP, Melo MT, dos Santos CE, Lima PR (1999) Utilization of the waste of sisal industry in the control of mosquito larvae. Rev Soc Bras Med Trop 32(1):23–29PubMedCrossRefGoogle Scholar
  35. Pushpalatha E, Muthukrishnan J (1995) Larvicidal activity of a few plant extracts against Culex quinquefasciatus and Anopheles stephensi. Indian J Malariol 32(1):14–23PubMedGoogle Scholar
  36. Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71:553–555PubMedCrossRefGoogle Scholar
  37. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008a) Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 102:867–873PubMedCrossRefGoogle Scholar
  38. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008b) Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res 102:981–988PubMedCrossRefGoogle Scholar
  39. Reddy PJ, Krishna D, Murthy US, Jamil K (1992) A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. Comput Appl Biosci 8:209–213PubMedGoogle Scholar
  40. Rivera-Arce E, Gattuso M, Alvarado R, Zárate E, Agüero J, Feria I, Lozoya X (2007) Pharmacognostical studies of the plant drug Mimosae tenuiflorae cortex. J Ethnopharmacol 113(3):400–408PubMedCrossRefGoogle Scholar
  41. Rongsriyam Y, Trongtokit Y, Komalamisra N, Sinchaipanich N, Apiwathnasorn C, Mitrejet A (2006) Formulation of tablets from the crude extract of Rhinacanthus nasutus (Thai local plant) against Aedes aegypti and Culex quinquefasciatus larvae: a preliminary study. Southeast Asian J Trop Med Public Health 7(2):265–271Google Scholar
  42. Sharma P, Mohan L, Srivastava CN (2006) Phytoextract-induced developmental deformities in malaria vector. Bioresour Technol 97(14):1599–1604PubMedCrossRefGoogle Scholar
  43. Siddiqui BS, Gulzar T, Mahmood A, Begum S, Khan B, Afshan F (2004) New insecticidal amides from petroleum ether extract of dried Piper nigrum L. whole fruits. Chem Pharm Bull (Tokyo) 52:1349–1352CrossRefGoogle Scholar
  44. Sun HX (2006) Adjuvant effect of Achyranthes bidentata saponins on specific antibody and cellular response to ovalbumin in mice. Vaccine 24(2006):3432–3439PubMedCrossRefGoogle Scholar
  45. Taylor WG, Fields PG, Elder JL (2004a) Insecticidal components from field pea extracts: isolation and separation of peptide mixtures related to pea albumin 1b. J Agric Food Chem 52(25):7491–7498PubMedCrossRefGoogle Scholar
  46. Taylor WG, Fields PG, Sutherland DH (2004b) Insecticidal components from field pea extracts: soyasaponins and lysolecithins. J Agric Food Chem 52(25):7484–7490PubMedCrossRefGoogle Scholar
  47. Valsaraj R, Pushpangadan P, Smitt UW, Andersen A, Nyman U (1997) Antimicrobial screening of selected medicinal plants from India. J Ethnopharmacol 58:75–83PubMedCrossRefGoogle Scholar
  48. WHO (1992) Vector resistance to pesticides. Fifteenth report of the WHO Expert Committee on Vector Biology and Control. WHO Tech Rep Ser 818:1–62Google Scholar
  49. WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides CTD/WHO PES/IC/96.1, p 69Google Scholar
  50. Wiesman Z, Chapagain BP (2006) Larvicidal activity of saponin containing extracts and fractions of fruit mesocarp of Balanites aegyptiaca. Fitoterapia 77(6):420–424PubMedCrossRefGoogle Scholar
  51. Yenesew A, Derese S, Midiwo JO, Heydenreich M, Peter MG (2003) Effect of rotenoids from the seeds of Millettia dura on larvae of Aedes aegypti. Pest Manag Sci 59(10):1159–1161PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • A. Bagavan
    • 1
  • A. A. Rahuman
    • 1
    Email author
  • C. Kamaraj
    • 1
  • Kannappan Geetha
    • 2
  1. 1.Unit of Bioactive Natural Products, Department of ZoologyC. Abdul Hakeem CollegeMelvisharamIndia
  2. 2.Department of ChemistryMuthurangam Govt. Arts CollegeVelloreIndia

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