Parasitology Research

, Volume 103, Issue 1, pp 171–174 | Cite as

Efficacy of female Culex quinquefasciatus with entomopathogenic fungus Fusarium pallidoroseum

  • Suman Sundar Mohanty
  • Kamaraju Raghavendra
  • Usha Rai
  • Aditya Prasad Dash
Original Paper


This study was conducted to isolate and identify natural entomopathogenic fungi from female Culex quinquefasciatus and to test their adulticidal activity. Field-collected female C. quinquefasciatus died early and were placed on a Saboraud’s dextrose agar plates for growth and isolation of natural entomopathogenic fungi. The plates were maintained in an incubator at 24 ± 2°C for 3 days. Four fungal species were isolated in two genera namely, Aspergillus and Fusarium. The identified fungal species were A. niger, A. flavus, A. nidulans var acristatus (ITCC-6327.04), and F. pallidoroseum (ITCC-6324.06). Adult bioassays were carried out using spore-impregnated paper in WHO-holding tubes. F. pallidoroseum was found to be more effective than the others. Exposure of C. quinquefasciatus to spores of A. flavus and A. niger for 4 h caused 5.53% and 5.51% mortality in the mosquitoes within a week, respectively. All the female C. quinquefasciatus were killed within 4 days of exposure to F. pallidoroseum at a concentration of 1.11 × 1010 conidia per m2. Significant difference of longevity was observed between the F. pallidoroseum-treated C. quinquefasciatus and control mosquitoes. The LT50 of F. pallidoroseum was 2.08 days for 4 h exposure to C. quinquefasciatus. Results of the present study confirm that F. pallidoroseum is one of the alternative biological control agents of adult mosquitoes.



The first author acknowledges the Council of Scientific and Industrial Research, New Delhi, for financial support in the form of Research Associateship.


  1. Balaraman K, Bheema Rao US, Rajagopalan PK (1979) Isolation of Metarrhizium anisopliae, Beauveria tenella and Fusarium oxysporum (Deuteromycetes) and their pathogenicity to Culex fatigans and Anopheles stephensi. Indian J Med Res 70:718–722PubMedGoogle Scholar
  2. Blanford S, Chan BHK, Jenkins N, Sim D, Turner RJ, Read AF, Thomas MB (2005) Fungal pathogen reduces potential for malaria transmission. Science 308:1638–1641PubMedCrossRefGoogle Scholar
  3. Clark TB, Kellen WR, Fukuda T, Lindegren JE (1968) Field and laboratory studies on the pathogenicity of the fungus Beauveria bassiana to three genera of mosquito. J Invertebr Pathol 11:1–7PubMedCrossRefGoogle Scholar
  4. Das PK, Pani SP, Krishnamoorthy K (2002) Prospects of elimination of lymphatic filariasis in India. ICMR Bull 32:41–54Google Scholar
  5. Federici BA (1981) Mosquito control by the fungi Culicinomyces, Lagenidium and Coelomomyces. In: Burges, HD (eds) Microbial control of pests and plant diseases 1970–1980. Academic, New York, pp 555–572Google Scholar
  6. Hargreaves K, Koekemoer LL, Brooke BD, Hunt RH, Mthembu J, Coetzee M (2000) Anopheles funestus resistant to pyrethroid insecticides in South Africa. Med Vet Entomol 14:181–189PubMedCrossRefGoogle Scholar
  7. Hargreaves K, Hunt RH, Brooke BD, Mthembu J, Weeto MM, Awolola TS, Coetzee M (2003) Anopheles arabiensis and An. quadriannulatus resistance to DDT in South Africa. Med Vet Entomol 17:417–422PubMedCrossRefGoogle Scholar
  8. Lacey CM, Lacey LA, Roberts DR (1988) Route of invasion and histopathology of Metarhizium anisopliae in Culex quinquefasciatus. J Invertebr Pathol 52:108–118PubMedCrossRefGoogle Scholar
  9. Mohanty SS, Prakash S (2008) Laboratory and field evaluation of the fungus Chrysosporium lobatum against the larvae of the mosquito Culex quinquefasciatus. Parasitol Res (in press), DOI 10.1007/s00436-007-0843-x
  10. Mourya DT, Iikal MA, Mishra AC, Jacob PG, Pant U, Ramanujam S, Mavale MS, Bhat HR, Dhanda V (1989) Isolation of Japanese encephalitis virus from mosquitoes collected in Karnataka state, India from 1985 to 1987. Trans R Soc Trop Med Hyg 83:550–552PubMedCrossRefGoogle Scholar
  11. Muangman D, Edelman R, Sullivan MJ, Gould DJ (1972) Experimental transmission of Japanese encephalitis virus by Culex fuscocephala. Am J Trop Med Hyg 21:482–486PubMedGoogle Scholar
  12. Munshi NA, Hussain B, Malik GN, Yousuf M, Fatima N (2008) Efficacy of entomopathogenic fungus Fusarium pallidoroseum (Cooke) Sacc. against gypsy moth (Lymantria obfuscata Walker). J Entomol 5:59–61CrossRefGoogle Scholar
  13. Paily KP, Hoti SL, Balaraman K (2006) Development of lymphatic filarial parasite Wuchereria bancrofti (Spirurida: Onchocercidae) in mosquito species (Diptera: Culicidae) fed artificially on microfilaremic blood. J Med Entomol 43:1222–1226PubMedCrossRefGoogle Scholar
  14. Ravindranath G, Kapadnis BP (1991) Isolation and extraction of Trichodermin from Fusarium pallidoroseum, a fungal pathogen of Anopheles stephensi. Indian J Microbiol 31:267–269Google Scholar
  15. Scholte EJ, Njiru BN, Smallegange RC, Takken W, Knols BGJ (2003) Infection of adult malaria (Anopheles gambiae s.s.) and filariasis (Culex quinquefasciatus) vectors with the entomopathogenic fungus Metarhizium anisopliae. Malar J 2:29PubMedCrossRefGoogle Scholar
  16. Scholte EJ, Nghabi K, Kihonda J, Takken W, Paaijmans K, Abdulla S, Killeen GF, Knols BGJ (2005) An entomopathogenic fungus for control of adult African malaria mosquitoes. Science 308:1641–1642PubMedCrossRefGoogle Scholar
  17. Scholte EJ, Takken W, Knols BGJ (2007) Infection of adult Aedes aegypti and Ae. albopictus mosquitoes with entomopathogenic fungus Metarhizium anisopliae. Acta Trop 102:151–158PubMedCrossRefGoogle Scholar
  18. Vyas N, Dua K, Prakash S (2007) Efficacy of Lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms. Parasitol Res 101:385–390PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Suman Sundar Mohanty
    • 1
  • Kamaraju Raghavendra
    • 1
  • Usha Rai
    • 2
  • Aditya Prasad Dash
    • 1
  1. 1.National Institute of Malaria ResearchIndian Council of Medical ResearchDelhiIndia
  2. 2.Department of Biotechnology, School of Life SciencesJiwaji UniversityGwaliorIndia

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