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Parasitology Research

, Volume 102, Issue 6, pp 1235–1242 | Cite as

In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis

  • Avijit Dutta
  • Debjani Sarkar
  • Ameenah Gurib-Fakim
  • Chitra Mandal
  • Mitali Chatterjee
Original Paper

Abstract

The leishmanicidal activity of Aloe vera leaf exudate (AVL) has been demonstrated in promastigotes and axenic amastigotes, but its effectiveness in animal models has not been evaluated. The presence of alkaloids, triterpenes, cyanidines, proanthocyanidines, tannins, and saponins in AVL was identified. Its effectiveness in four Leishmania donovani strains was studied both in promastigotes (IC50 ranged from 70–115 μg/ml) and amastigotes (IC50 ranged from 3.1–11.4 μg/ml). In amastigotes, the killing by AVL was facilitated through its induction of nitric oxide in leishmania-infected macrophages. The safety index was good as AVL up to 300 μg/ml remained non-toxic to monocytes and macrophages. In a L. donovani BALB/c mouse model, oral or subcutaneous administration of AVL (15 mg/kg body weight × 5 days) reduced parasitemia by >90% in the liver, spleen, and bone marrow without impairment of hepatic and renal functions. Collectively, we conclude that AVL shows promising antileishmanial activity and may provide a new lead agent in the treatment of Leishmaniasis.

Keywords

Nitric Oxide Visceral Leishmaniasis Leishmaniasis Miltefosine Murine Peritoneal Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The work received financial support from Council of Scientific and Industrial Research and Department of Biotechnology, Government of India. Avijit Dutta received a Senior Research Fellowship from Indian Council of Medical Research. We gratefully acknowledge the receipt of strains from the Leishmania Bank (Dr. Swadesh Duttagupta, Indian Institute of Chemical Biology, Kolkata) and Dr. Neeloo Singh, Central Drug Research Institute, Lucknow.

References

  1. Akendengue B, Ngou-Milama E, Laurens A, Hocquemiller R (1999) Recent advances in the fight against leishmaniasis with natural products. Parasite 6:3–8PubMedGoogle Scholar
  2. Ali MI, Shalaby NM, Elgamal MH, Mousa AS (1999) Antifungal effects of different plant extracts and their major components of selected Aloe species. Phytother Res 13:401–407PubMedCrossRefGoogle Scholar
  3. Bredt DS, Synder SH (1994) Nitric oxide: a physiologic messenger molecule. Ann Rev Biochem 63:175–195PubMedCrossRefGoogle Scholar
  4. Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M (2007) Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5:873–882PubMedCrossRefGoogle Scholar
  5. Desjeux P (2004) Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27:305–318PubMedCrossRefGoogle Scholar
  6. Dupouy-Camet J (2004) New drugs for the treatment of human parasitic protozoa. Parasitologia 46:81–84Google Scholar
  7. Dutta A, Bandyopadhyay S, Mandal C, Chatterjee M (2005) Development of a modified MTT assay for screening antimonial resistant field isolates of Indian visceral leishmaniasis. Parasitol Int 54:119–122PubMedCrossRefGoogle Scholar
  8. Dutta A, Bandyopadhyay S, Mandal C, Chatterjee M (2007a) Aloe vera leaf exudate induces a caspase independent cell death in Leishmania donovani promastigotes. J Med Microbiol 56:629–636PubMedCrossRefGoogle Scholar
  9. Dutta A, Mandal G, Mandal C, Chatterjee M (2007b) In vitro antileishmanial activity of Aloe vera leaf exudate: a potential herbal therapy in leishmaniasis. Glyconj J 24:81–86CrossRefGoogle Scholar
  10. Ferro VA, Bradbury F, Cameron P, Shakir E, Rahman SR, Stimson WH (2003) In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller. Antimicrob Agents Chemother 47:1137–1139PubMedCrossRefGoogle Scholar
  11. Ghosh MN (2005) Fundamentals of experimental pharmacology, 3rd edn. Hilton & Company, Kolkata, pp 190–197Google Scholar
  12. Guerin PJ, Olliaro P, Sundar S, Boelaert M, Croft SL, Desjeux P, Wasunna MK, Bryceson AD (2002) Visceral Leishmaniasis: current status of control, diagnosis and treatment and a proposed research and development agenda. Lancet Infect Dis 2:494–501PubMedCrossRefGoogle Scholar
  13. Handman E (2001) Leishmaniasis: current status of vaccine development. Clin Microbiol Rev 14:229–243PubMedCrossRefGoogle Scholar
  14. Harborne JB (1973) Phytochemical methods. A guide to modern techniques of plant analysis, 1st edn. Chapman and Hall, LondonGoogle Scholar
  15. Holzmuller P, Sereno D, Cavaleyra M, Mangot I, Daulouede S, Vincendeau P, Lemesre JL (2002) Nitric oxide-mediated proteasome-dependent oligonucleosomal DNA fragmentation in Leishmania amazonensis amastigotes. Infect Immun 70:3727–3735PubMedCrossRefGoogle Scholar
  16. Kayser O, Kinderlen AF, Croft SL (2003) Natural products as antiparasitic drugs. Parasitol Res 90:S55–S62PubMedCrossRefGoogle Scholar
  17. Maes L, Vandenberghe D, Germonprez N, Quirijnen L, Cos P, Vanden Berghe D, Van Puyvelde L (2004) In vitro and in vivo activities of a triterpenoid saponin extract (PX-6518) from the plant Maesa balansae against visceral leishmania species. Antimicrob Agents Chemother 48:130–136PubMedCrossRefGoogle Scholar
  18. Newman DJ, Cragg GM, Snader KM (2000) The influence of natural products upon drug discovery. Nat Prod Rep 17:215–234PubMedCrossRefGoogle Scholar
  19. Pal S, Mandal A, Duttagupta S (2001) Studies on stibanate resistant Leishmania donovani isolates of Indian origin. Ind J Exp Biol 39:249–254Google Scholar
  20. Paris C, Loiseau PM, Bories C, Breard J (2004) Miltefosine induces apoptosis-like death in Leishmania donovani promastigotes. Antimicrob Agents Chemother 48:852–859PubMedCrossRefGoogle Scholar
  21. Reynolds T, Dweck AC (1999) Aloe vera leaf-gel: a review update. J Ethnopharmacol 68:3–37PubMedCrossRefGoogle Scholar
  22. Roberts WL, Rainey PM (1993) Antileishmanial activity of sodium stibogluconate fractions. Antimicrob Agents Chemother 37:1842–1846PubMedGoogle Scholar
  23. Sacks D, Noben-Trauth N (2002) The immunology of susceptibility and resistance to Leishmania major in mice. Nat Rev Immunol 2:845–858PubMedCrossRefGoogle Scholar
  24. Singh N (2002) Is there true Sb(V) resistance in Indian Kala azar field isolates? Curr Science 83:210–211Google Scholar
  25. Stenger S, Donhauser N, Thuring H, Rollinghoff M, Boqdan C (1996) Reactivation of latent leishmaniasis by inhibition of inducible nitric oxide synthase. J Exp Med 183:1501–1514PubMedCrossRefGoogle Scholar
  26. Sundar S (2001) Drug resistance in Indian visceral leishmaniasis. Trop Med Int Health 6:849–854PubMedCrossRefGoogle Scholar
  27. The US Pharmacopeia (1995) Bacterial endotoxin test, 23rd revision. Mack Publishing, EastonGoogle Scholar
  28. World Health Organization (2000) A report of the consultation meeting on traditional and modern medicine: harmonizing two approaches, 22–26 November 1999, Beijing, China., West Pacific RegionGoogle Scholar
  29. Yardley V, Croft SL (2000) A comparison of the activities of three amphotericin B lipid formulations against experimental visceral and cutaneous leishmaniasis. Int J Antimicrob Agents 13:70–75CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Avijit Dutta
    • 1
    • 4
  • Debjani Sarkar
    • 2
  • Ameenah Gurib-Fakim
    • 3
  • Chitra Mandal
    • 1
  • Mitali Chatterjee
    • 2
  1. 1.Department of Infectious Disease and ImmunologyIndian Institute of Chemical BiologyKolkataIndia
  2. 2.Department of PharmacologyInstitute of Postgraduate Medical Education and ResearchKolkataIndia
  3. 3.Department of ChemistryUniversity of MauritiusReduitMauritius
  4. 4.Division of Infectious Disease, Department of MedicineChang Gung University School of Medicine and HospitalKweishanTaiwan

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