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Parasitology Research

, Volume 102, Issue 5, pp 1013–1020 | Cite as

Morphometric and molecular characteristics of Labeonema synodontisi n. comb. (Nematoda: Atractidae) from the West African fishes

  • Božena KoubkováEmail author
  • Vlastimil Baruš
  • Iveta Hodová
  • Andrea Šimková
Original Paper

Abstract

Numerous nematodes were found in the rectum of three fish species Synodontis ocellifer, S. nigrita, and S. schall (Mochokidae, Siluriformes) from the Gambia River and Mare Simenti, National Park Niokolo Koba, Senegal. A nematode species Raillietnema synodontisi Vassiliadès, 1973 (host S. ocellifer), is redescribed using morphometric (including scanning electron microscopy) and molecular characteristics and transferred into the genus Labeonema Puylaert, 1970. It is morphologically and metrically similar to Labeonema intermedium Puylaert, 1970, the other congeneric species (L. bainae Baker, 1982; L. bakeri Van Waerebeke, Chabaud, Bain et Georges, 1988; and L. africanum Moravec et Van As, 2004) differ from them either by the spicule and gubernaculum lengths, distribution and number of pre-cloacal papillae, position of the vulva, as well as hosts and geographical distribution. The partial sequences of small ribosomal subunit rDNA of L. synodontisi were analyzed and compared with other nematode sequences. Molecular analyses seem to support the position of this nematode species based on the morphological observation.

Keywords

Nematode Species Anterior Extremity Cephalic Papilla Genital Papilla Digital Image Analysis System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank the management of the Senegal National Parks for logistic support and the guide Amadou Demba Ndiaye for assistance. They would also like to thank Martina Vyskočilová, from the Department of Botany and Zoology, Faculty of Science MU, Brno, Czech Republic for DNA analysis. Thanks are also due to Mr. Jimmy Cassone, Chargé des collections d'Helminthes Muséum National d'Histoire Naturelle, Paris for the loan of the type specimens of R. synodontisi Vassiliadès, 1973.

This study was partly supported by grant No. IAA6093404 (B.K., V.B., A.S.) from the Grant Agency of the Academy of Science of the Czech Republic and by the Research Project of the Masaryk University No. MSM 0021622416 and Ichthyoparasitology Research Centre of the Ministry of Education, Youth and Sports of the Czech Republic (LC 522) (B.K., I.H., A.S.).

References

  1. Baker MR (1982) Systematic relationships of the Atractidae and Cosmocercidae (Nematoda: Cosmocercoidea): two new atractids parasitic in amphibian and fish. Can J Zool 60:2395–2402CrossRefGoogle Scholar
  2. Boomker J (1994) Parasites of South African freshwater fishes. IV. Nematode parasites of some fish species in the Kruger National Park. Onderstepoort J Vet Res 61:35–43PubMedGoogle Scholar
  3. Floyd R, Abebe E, Papert A, Blaxter M (2002) Molecular barcodes for soil nematode identification. Mol Ecol 11:839–850PubMedCrossRefGoogle Scholar
  4. Floyd RM, Rogers AD, Lambshead JD, Smith C (2005) Nematode-specific PCR primers for the 18S small subunit rRNA gene. Mol Ecol Notes 5:611–612CrossRefGoogle Scholar
  5. Hillis DM, Dixon MT (1991) Ribosomal DNA molecular evolution and phylogenetic inference. Q Rev Biol 66:410–453CrossRefGoogle Scholar
  6. Khalil LF, Polling L (1997) Checklist of the helminth parasites of African freshwater fishes, 2nd edn. Pietersburg: University of the North, Republic of South AfricaGoogle Scholar
  7. Moravec F, Van As JG (2004) Nematodes from the squeaker fishes Synodontis nigromaculatus and S. vanderwaali from the Okavango River, Botswana, including three new species. Syst Parasitol 59:169–187PubMedCrossRefGoogle Scholar
  8. Moravec F, Řehulka J (1987) First record of the cosmocercoid nematode Raillietnema synodontisi Vassiliadès, 1973 from aquarium-reared upside-down catfish Synodontis eupterus Boulenger. Folia Parasitol 34:163–164PubMedGoogle Scholar
  9. Moravec F, Salgado-Maldonado G, Pineda-López R (1993) Raillietnema kritscheri sp. n. (Nematoda: Cosmocercidae) from Cichlasoma spp. (Pisces) from Mexico. Ann Naturhist Mus Wien 94/95 B:35–40Google Scholar
  10. Puylaert FA (1970) Description de Labeonema intermedia gen. n., n. sp., parasite d’un Labeo (Pisces—Cyprinidae) (Atractidae—Nematoda—Vermes). Rev Zool Bot Afr 82:370–376Google Scholar
  11. Van Waerebeke D, Chabaud AG, Bain O, Georges AJ (1988) Deux nouvelles nématodes parasites de poissons de l'Oubangui. B Mus Natl Hist Nat 10:519–527Google Scholar
  12. Vassiliadès G (1973) Un nouveau Raillietnema (Nematoda; Cosmocercoidea) parasite de poisson, au Sénégal. Bull Inst Fond Afr Noire Sér A 35:515–821Google Scholar
  13. Vassiliadès G (1975) Nématodes parasites des poissons déau donce de la République du Sénégal. Bull Inst Fond Afr Noire Sér A 37:605–681Google Scholar
  14. Vassiliadès G, Troncy PM (1974) Nématode parasites des poissons du bassin tchadien. Bull Inst Fond Afr Noire Sér A 36:670–681Google Scholar
  15. Wijová M, Moravec F, Horák A, Modrý D, Lukeš J (2005) Phylogenetic position of Dracunculus medinensis and some related nematodes inferred from 18S rRNA. Parasitol Res 96:133–135PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Božena Koubková
    • 1
    Email author
  • Vlastimil Baruš
    • 2
  • Iveta Hodová
    • 1
  • Andrea Šimková
    • 1
  1. 1.Department of Botany and Zoology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Institute of Vertebrate BiologyAcademy of Sciences of the Czech RepublicBrnoCzech Republic

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