Parasitology Research

, Volume 102, Issue 5, pp 867–873 | Cite as

Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

  • A. Abdul Rahuman
  • Geetha Gopalakrishnan
  • P. Venkatesan
  • Kannappan Geetha
Original Paper

Abstract

Larvicidal activity of ethyl acetate, butanol, and petroleum ether extracts of five species of Euphorbiaceae plants, Jatropha curcas, Pedilanthus tithymaloides, Phyllanthus amarus, Euphorbia hirta, and Euphorbia tirucalli, were tested against the early fourth instar larvae of Aedes aegypti L. and Culex quinquefasciatus (Say). The larval mortality was observed after 24 h of exposure. All extracts showed low larvicidal effects; however, the highest larval mortality was found in petroleum ether extract. The LC50 value of petroleum ether extracts of J. curcas, P. tithymaloides, P. amarus, E. hirta, and E. tirucalli were 8.79, 55.26, 90.92, 272.36, and 4.25 ppm, respectively, against A. aegypti and 11.34, 76.61, 113.40, 424.94, and 5.52 ppm, respectively, against C quinquefasciatus. Of the various ratios tested, the petroleum ether extracts of J. curcas and E. tirucalli were observed to be more efficient than the other plant extracts. It is, therefore, suggested that E. tirucalli can be applied as an ideal potential larvicide against A. aegypti and C. quinquefasciatus. This is an ideal ecofriendly approach for the control of the dengue vector, A. aegypti, and the lymphatic filariasis vector, C. quinquefasciatus.

References

  1. Abreu P, Matthew S, Gonzalez T, Costa D, Segundo MA, Fernandes E (2006) Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides. Life Sci 78:1578–1585PubMedCrossRefGoogle Scholar
  2. Adamu HM, Abayeh OJ, Agho MO, Abdullahi AL, Uba A, Dukku HU, Wufem BM (2005) An ethnobotanical survey of Bauchi State herbal plants and their antimicrobial activity. J Ethnopharmacol 99:1–4PubMedCrossRefGoogle Scholar
  3. Amer A, Mehlhorn H (2006) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477PubMedCrossRefGoogle Scholar
  4. Amer A, Mehlhorn H (2006) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472PubMedCrossRefGoogle Scholar
  5. Bernhard L, Bernhard P, Magnussen P (2003) Management of patients with lymphoedema caused by filariasis in North-eastern Tanzania: alternative approaches. Physiotherapy 89:743–749CrossRefGoogle Scholar
  6. Bigi MF, Torkomian VL, de Groote ST, Hebling MJ, Bueno OC, Pagnocca FC, Fernandes JB, Vieira PC, da Silva MF (2004) Activity of Ricinus communis (Euphorbiaceae) and ricinine against the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera: Formicidae) and the symbiotic fungus Leucoagaricus gongylophorus. Pest Manag Sci 60(9):933–938PubMedCrossRefGoogle Scholar
  7. Brasileiro BG, Pizziolo VR, Raslan DS, Jamal CM, Silveira D (2006) Antimicrobial and cytotoxic activities screening of some Brazilian medicinal plants used in Governador Valadares district. RBCF Rev Bras Cienc Farm 42(2):195–202Google Scholar
  8. Chen L (1991) Polyphenols from leaves of Euphorbia hirta L. Zhongguo Zhongyao Zazhi 16(1):38–39PubMedGoogle Scholar
  9. Civelek HS, Weintraub PG (2004) Effects of two plant extracts on larval leafminer Liriomyza trifolii (Diptera: Agromyzidae) in tomatoes. J Econ Entomol 97(5):1581–6PubMedCrossRefGoogle Scholar
  10. Dhar SN, Ray SM, Roy A, Dutta SK (1988) Oral anti-inflammatory activity of pedilanthain—a new proteolytic enzyme from Pedilanthus tithymaloides. Indian J Pharm Sci 50:281–283Google Scholar
  11. Diallo D, Sogn C, Samake FB, Paulsen BS, Michaelsen TE, Keita A (2002) Wound healing plants in Mali, the Bamako region. An ethnobotanical survey and complement fixation of water extracts from selected plants. Pharm Biol 40:117–128CrossRefGoogle Scholar
  12. Evans FJ, Soper CJ (1978) The tigliane, daphnane and ingenane diterpenes, their chemistry, distribution and biological activities. A review. Lloydia 41:193–233PubMedGoogle Scholar
  13. Filho ECO, Paumgartten FJ (2000) Toxicity of Euphorbia milii latex and niclosamide to snails and nontarget aquatic species. Ecotoxicol Environ Saf 46(3):342–350CrossRefGoogle Scholar
  14. Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquitoes bites. N Engl J Med 347:13–18PubMedCrossRefGoogle Scholar
  15. Gálvez J, Crespo ME, Jiménez J, Suárez A, Zarzuelo A (1993) Antidiarrhoeic activity of quercitrin in mice and rats. J Pharm Pharmacol 45(2):157–159PubMedGoogle Scholar
  16. Georges K, Jayaprakasam B, Dalavoy SS, Nair MG (2007) Pest-managing activities of plant extracts and anthraquinones from Cassia nigricans from Burkina Faso. Bioresour Technol (in press)Google Scholar
  17. Goonasekara MM, Gunawardhana VK, Jayaseana K, Mohammed SG, Balasubramaniam S (1995) Pregnancy terminating effect of Jatropha curcas in rats. J. Ethnopharmacol 47:117–123CrossRefGoogle Scholar
  18. Hales S, Wet ND, Maindonald J, Woodward A (2002) Potential effect of population and climate changes on global distribution of dengue fever: an empirical model. Lancet 360:830–834PubMedCrossRefGoogle Scholar
  19. Harve G, Kamath V (2004) Larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against Aedes aegypti. Indian J Exp Biol 42(12):1216–1219PubMedGoogle Scholar
  20. Heller J (1996) Promoting the conservation and use of under utilized and neglected crops. 1. Physic nut: Jatropha curcas L. International Plant Genetic Resources Institute, RomeGoogle Scholar
  21. Inserra RN, Dunn RAM, Sorley R, Langdow KR, Richmer AY (1989) Weed hosts of Rotylenchulus reniformis in ornamental nurseries of S. Florida. Weed Abstr 39:444Google Scholar
  22. Irungu LW, Mwangi RW (1995) Effects of a biologically active fraction from Melia volkensii on Culex quinquefasciatus. Insect Sci Appl 16:159–162Google Scholar
  23. Iwu MM (1993) Handbook of African medicinal plants. CRC, Boca Raton, FL, pp 24–33Google Scholar
  24. Karmegam N, Sakthivadivel M, Anuradha V, Daniel T (1997) Indigenous-plant extracts as larvicidal agents against Culex quinquefasciatus Say. Bioresour Technol 59(2–3):137–140CrossRefGoogle Scholar
  25. Khanna S, Srivastava CN, Srivastava MM, Srivastava S (2003) Insecticidal activity of the plant Phyllanthus amarus against Tribolium castaneum. J Environ Biol 24(4):391–394PubMedGoogle Scholar
  26. Kiemer AK, Hartung T, Huber C, Vollmar AM (2003) Phyllanthus amarus has anti-inflammatory potential by inhibition of iNOS, COX-2, and cytokines via the NF-kB pathway. J Hepatol 38:289–297PubMedCrossRefGoogle Scholar
  27. Komalamisra N, Trongtokit Y, Rongsriyam Y, Apiwathnasorn C (2005) Screening for larvicidal activity in some Thai plants against four mosquito vector species. Southeast Asian J Trop Med Public Health 36(6):1412–1422PubMedGoogle Scholar
  28. Lima MG, Maia IC, Sousa BD, Morais SM, Freitas SM (2006) Effect of stalk and leaf extracts from Euphorbiaceae species on Aedes aegypti (Diptera, Culicidae) larvae. Rev Inst Med Trop Sao Paulo 48(4):211–214PubMedGoogle Scholar
  29. Liu SY, Sporer F, Wink M, Jourdane J, Henning R Li YL, Ruppel A (1997) Anthraquinones in Rheum palmatum and Rumex dentatus (Polygonaceae), and phorbol esters in Jatropha curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails Oncomelania, Biomphalaria, and Bulinus. TM IH Trop Med Int Health 2:179–188CrossRefGoogle Scholar
  30. Luize PS, Ueda-Nakamura T, Zimmermann A, Vidoti GJ, Dias Filho BP, Morgado-Diaz JA, Nakamura CV (2003) Ultrastructural alterations induced by AZ-7, a compound from Pedilanthus tithymaloides, on Amastigote forms of Trypanosoma cruzi. Acta Microsc 12:319–320Google Scholar
  31. MacNeil A, Sumba OP, Lutzke ML, Moormann A, Rochford R (2003) Activation of the Epstein–Barr virus lytic cycle by the latex of the plant Euphorbia tirucalli. Br J Cancer 88(10):1566–1569PubMedCrossRefGoogle Scholar
  32. Mansour SA, Messeha SS, el-Gengaihi SE (2000) Botanical biocides. 4. Mosquitocidal activity of certain Thymus capitatus constituents. J Nat Toxins 9(1):49–62PubMedGoogle Scholar
  33. Meshram PB, Kulkarni N, Joshi KC (1996) Antifeedant activity of Azadirachta indica and Jatropha curcas against Papilio demoleus L. J Environ Biol 17:295–298Google Scholar
  34. Mohan L, Sharma P, Srivastava CN (2005) Evaluation of Solanum xanthocarpum extracts as mosquito larvicides. J Environ Biol 26(2):399–401PubMedGoogle Scholar
  35. Morais SM, Cavalcanti ES, Bertini LM, Oliveira CL, Rodrigues JR, Cardoso JH (2006) Larvicidal activity of essential oils from Brazilian Croton species against Aedes aegypti L. J Am Mosq Control Assoc 22(1):161–164PubMedCrossRefGoogle Scholar
  36. O’Keefe BR (2001) Biologically active proteins from natural product extracts. J Nat Prod 64:1373–1381PubMedCrossRefGoogle Scholar
  37. Parekh J, Chanda VS (2007) In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol 31:53–58Google Scholar
  38. Pushpalatha E, Muthukrishnan J (1995) Larvicidal activity of a few plant extracts against Culex quinquefasciatus and Anopheles stephensi. Indian J Malariol 32(1):14–23PubMedGoogle Scholar
  39. Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71:553–555PubMedCrossRefGoogle Scholar
  40. Rajeshkumar NV, Joy KL, Kuttan G, Ramsewak RS, Nair MG, Kuttan R (2002) Antitumour and anticarcinogenic activity of Phyllanthus amarus extract. J Ethnopharmacol 81:17–22PubMedCrossRefGoogle Scholar
  41. Ramaiah KD, Das PK, Michael E, Guyatt H (2000) The economic burden of lymphatic filariasis in India. Parasitol Today 16(6):251–253PubMedCrossRefGoogle Scholar
  42. Rao MS, Tewari RP, Parvatha Reddy P, Pandey M (1991) Comparative efficacy of Glyricidia maculata, Pedilanthus tithymaloides and carbofuran in the management of nematode, Aphelenchoides sacchari. In Proceedings of the National Symposium on Mushrooms, Trivandrum, KAU, pp 245–247Google Scholar
  43. Rasheed M, Afshan F, Tariq RM, Siddiqui BS, Gulzar T, Mahmood A, Begum S, Khan B (2005) Phytochemical studies on the seed extract of Piper nigrum Linn. National Product Research 19(7):703–712CrossRefGoogle Scholar
  44. Ratnadass A, Hamada MA, Traoré S, Cissé S, Sidibé B (2001) On-farm development and testing of IPM packages for control of sorghum head-bugs in Mali. Med Fac Landbouww Univ Gent 66(2a):315–324Google Scholar
  45. Reddy PJ, Krishna D, Murthy US, Jamil K (1992) A microcomputer FORTRAN program for rapid determination of lethal concentration of biocides in mosquito control. CABIOS 8:209–213PubMedGoogle Scholar
  46. Reuben R (1987) Feeding and reproduction in mosquitoes. Proc Indian Acad Sci Anim Sci 96:275–280CrossRefGoogle Scholar
  47. Sakthivadivel M, Thilagavathy D (2003) Larvicidal and chemosterilant activity of the acetone fraction of petroleum ether extract from Argemone mexicana L seed. Bioresour Technol 89(2):213–216PubMedCrossRefGoogle Scholar
  48. Saxena RC, Dixit OP, Sukumaran P (1992) Laboratory assessment of indigenous plant extracts for anti-juvenile hormone activity in Culex quinquefasciatus. Indian J Med Res 95:204–206PubMedGoogle Scholar
  49. Seshagirirao K (1995) Purification and partial characterization of a lectin from Pedilanthus tithymaloides latex. Biochem Arch 11:197–201Google Scholar
  50. Sharma M, Saxena RC (1994) Phytotoxicologial evaluation of Tegetes erectes on aquatic stages of Anopheles stephensi. Indian J. Malariol 31:21–26PubMedGoogle Scholar
  51. Sharma N, Trivedi PC (2002) Screening of leaf extracts of some plants for their nematicidal and fungicidal properties against Meloidogyne incognita and Fusarium oxysporum. Asian J Exp Sci 16:21–28Google Scholar
  52. Sharma P, Mohan L, Srivastava CN (2004) Larval susceptibility of Ajuga remota against anopheline and culicine mosquitos. Southeast Asian J Trop Med Public Health 35(3):608–610PubMedGoogle Scholar
  53. Sharma P, Mohan L, Srivastava CN (2006) Phytoextract-induced developmental deformities in malaria vector. Bioresour Technol 97(14):1599–1604PubMedCrossRefGoogle Scholar
  54. Siddiqui BS, Gulzar T, Mahmood A, Begum S, Khan B, Afshan F (2004) New insecticidal amides from petroleum ether extract of dried Piper nigrum L. whole fruits. Chem Pharm Bull 52(11):1349–1352PubMedCrossRefGoogle Scholar
  55. Taubes G (1997) A mosquito bites back. New York Times Magazine 24 August, pp 40–46Google Scholar
  56. Taubitz W, Cramer JP, Kapaun A, Pfeffer M, Drosten C, Dobler G, Burchard GD, Löscher T (2007) Chikungunya fever in travelers: clinical presentation and course. Clin Infect Dis 45(1):1–4CrossRefGoogle Scholar
  57. Thyagarajan SP, Subramanian S, Thirunalasundari T, Venkateswaran PS, Blumberg BS (1988) Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet 2:764–766PubMedCrossRefGoogle Scholar
  58. Tiwari S, Singh A (2005) Alterations in carbohydrates and the protein metabolism of the harmful freshwater vector snail Lymnaea acuminata induced by the Euphorbia tirucalli latex extract. Environ Res 99(3):378–386PubMedCrossRefGoogle Scholar
  59. Unander DW, Venkateswaran PS, Millman I, Bryan HH, Blumberg BS (1990) Phyllanthus species: sources of new antiviral compounds. In: Janick J, Simon JE (eds) Advances in new crops. Timber, Portland, USA, pp 518–521Google Scholar
  60. Unander DW, Webster GL, Blumberg BS (1991) Uses and bioassays in Phyllanthus (Euphorbiaceae): a compilation II. The subgenus Phyllanthus. J Ethnopharmacol 34:97–133PubMedCrossRefGoogle Scholar
  61. Upasani SM, Kotkar HM, Mendki PS, Maheshwari VL (2003) Partial characterization and insecticidal properties of Ricinus communis L foliage flavonoids. Pest Manag Sci 59(12):1349–1354PubMedCrossRefGoogle Scholar
  62. Vidotti GJ, Zimmermann A, Sarragiotto MH, Nakamura CV, Filho BPD (2006) Antimicrobial and phytochemical studies on Pedilanthus tithymaloides. Fitoterapia 77:43–46PubMedCrossRefGoogle Scholar
  63. Wei H, Hou Y, Yang G, Fu J, You M (2005) Evaluation of non-host plant ethanol extracts against Plutella xylostella population. Yingyong Shengtai Xuebao 16(6):1086–1089PubMedGoogle Scholar
  64. Wei H, Hou Y, Yang G, You M (2004) Repellent and antifeedant effect of secondary metabolites of non-host plants on Plutella xylostella. Yingyong Shengtai Xuebao 15(3):473–476PubMedGoogle Scholar
  65. WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides. CTD/WHO PES/IC/96.1, p 69Google Scholar
  66. Wink M, Koschmieder C, Sauerwein M, Sporer F (1997) Phorbol esters of Jatropha curcas—biological activities and potential applications. In: Gübitz GM, Mittelbach M, Trabi M (eds) Biofuel and industrial products from Jatropha curcas. Dbv-Verlag Univ., GrazGoogle Scholar
  67. Wititsuwannakul R, Wititsuwannakul D, Sakulborirug C (1998) A lectin from the bark of the rubber tree (Hevea brasiliensis). Phytochemistry 47:183–187PubMedCrossRefGoogle Scholar
  68. Yadav R, Srivastava VK, Chandra R, Singh A (2002) Larvicidal activity of latex and stem bark of Euphorbia tirucalli plant on the mosquito Culex quinquefasciatus. J Commun Dis 34(4):264–269PubMedGoogle Scholar
  69. Yeh SF, Hong CY, Huang YL, Liu TY, Choo KB, Chou CK (1993) Effect of an extract from Phyllanthus amarus on hepatitis B surface antigen gene expression in human hepatoma cells. Antivir Res 20:185–192PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • A. Abdul Rahuman
    • 1
  • Geetha Gopalakrishnan
    • 2
  • P. Venkatesan
    • 3
  • Kannappan Geetha
    • 4
  1. 1.Unit of Bioactive Natural Products, Department of ZoologyC. Abdul Hakeem CollegeMelvisharamIndia
  2. 2.Center for Natural ProductsSPIC Science FoundationChennaiIndia
  3. 3.Department of ZoologyLoyola CollegeChennaiIndia
  4. 4.Department of ChemistryMuthurangam Government Arts CollegeVelloreIndia

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