Opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro
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The tegumental changes in adult Opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (SEM). We exposed O. viverrini to tribendimidine at a concentration of 10 μg/ml for 4 h. In addition, hamsters were treated with a single 400 mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96 h post-treatment. SEM analysis of the flukes incubated in vitro showed only mild damage of the tegument. Twenty-four hours post-treatment of hamsters, extensive disruption such as sloughing, furrowing, or blebbing of the tegument was evident, which did not increase in severity 48–72 h post-treatment. Ninety-six hours after tribendimidine administration, the majority of flukes had been expelled. A single 400 mg/kg oral dose of tribendimidine administered to O. viverrini-infected hamsters yielded a worm burden reduction of 63.0%. In conclusion, our experiments confirm that tribendimidine possesses interesting trematocidal properties, which warrant additional investigations and provide further data for subsequent clinical trials.
KeywordsScanning Electron Microscope Observation Praziquantel Ventral Sucker Oral Sucker Worm Burden
We thank Mr. Gianni Morson at the Centre for Microscopy, University of Basel for expert help with the SEM studies. J. Keiser (project no. PPOOA-114941), J. Utzinger (project no. PPOOB-102883) and P. Odermatt (project no. NF3270B0-110020) are grateful to the Swiss National Science Foundation for financial support.
- Haswell-Elkins M, Levri E (2003) Food-borne trematodes. In: Cook GC, Zumla AL (eds) Manson’s tropical diseases, 21st edn. Saunders, London, pp. 1471–1486Google Scholar
- Keiser J, Morson G (2008) Fasciola hepatica: tegumental alterations in adult flukes following in vitro and in vivo administration of artesunate and artemether. Exp Parasitol 118:228–237Google Scholar
- Keiser J, Thiemann K, Endriss Y, Utzinger J (2008) Strongyloides ratti: in vitro and in vivo activity of tribendimidine. PLoS Negl Trop Dis 2:e136Google Scholar
- Mehlhorn H, Kojima S, Rim HJ, Ruenwongsa P, Andrews P, Thomas H, Bunnag B (1983) Ultrastructural investigations on the effects of praziquantel on human trematodes from Asia: Clonorchis sinensis, Metagonimus yokogawai, Opisthorchis viverrini, Paragonimus westermani and Schistosoma japonicum. Arzneimittelforschung 33:91–98PubMedGoogle Scholar
- Ren HN, Cheng BZ, Zhuang ZN (1987) Experimental therapeutic efficacy of a new anti-hookworm drug-tribendimidine. Chin J Parasitol Parasit Dis 5:262–264 (in Chinese)Google Scholar
- Sirisinha S, Puengtomwatanakul S, Sobhon P, Saitongdee P, Wongpayabal P, Mitranonde V, Radomyos P, Bunnag D, Harinasuta T (1984) Alterations of the surface tegument of Opisthorchis viverrini exposed to praziquantel in vitro and in vivo. Southeast Asian J Trop Med Public Health 15:95–103PubMedGoogle Scholar