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Parasitology Research

, Volume 102, Issue 4, pp 771–776 | Cite as

Opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro

  • Jennifer Keiser
  • Jürg Utzinger
  • Shu-Hua Xiao
  • Peter Odermatt
  • Smarn Tesana
Original Paper

Abstract

The tegumental changes in adult Opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (SEM). We exposed O. viverrini to tribendimidine at a concentration of 10 μg/ml for 4 h. In addition, hamsters were treated with a single 400 mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96 h post-treatment. SEM analysis of the flukes incubated in vitro showed only mild damage of the tegument. Twenty-four hours post-treatment of hamsters, extensive disruption such as sloughing, furrowing, or blebbing of the tegument was evident, which did not increase in severity 48–72 h post-treatment. Ninety-six hours after tribendimidine administration, the majority of flukes had been expelled. A single 400 mg/kg oral dose of tribendimidine administered to O. viverrini-infected hamsters yielded a worm burden reduction of 63.0%. In conclusion, our experiments confirm that tribendimidine possesses interesting trematocidal properties, which warrant additional investigations and provide further data for subsequent clinical trials.

Keywords

Scanning Electron Microscope Observation Praziquantel Ventral Sucker Oral Sucker Worm Burden 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Mr. Gianni Morson at the Centre for Microscopy, University of Basel for expert help with the SEM studies. J. Keiser (project no. PPOOA-114941), J. Utzinger (project no. PPOOB-102883) and P. Odermatt (project no. NF3270B0-110020) are grateful to the Swiss National Science Foundation for financial support.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jennifer Keiser
    • 1
  • Jürg Utzinger
    • 2
  • Shu-Hua Xiao
    • 3
  • Peter Odermatt
    • 2
  • Smarn Tesana
    • 4
  1. 1.Department of Medical Parasitology and Infection BiologySwiss Tropical InstituteBaselSwitzerland
  2. 2.Department of Public Health and EpidemiologySwiss Tropical InstituteBaselSwitzerland
  3. 3.National Institute of Parasitic DiseasesChinese Center for Disease Control and PreventionShanghaiPeople’s Republic of China
  4. 4.Department of Parasitology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand

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