Trypanocidal activity of extracts and compounds from the stem bark of Anogeissus leiocarpus and Terminalia avicennoides
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The antitrypanosomal activity of methanolic extracts of Anogeissus leiocarpus and Terminalia avicennoides were evaluated in vitro against four strains of Trypanosoma species with minimum inhibitory concentration (MIC) value range of 12.5–50 mg/ml. Successive fractionations of the two plant extracts in water, butanol and ethyl acetate gave a range of activity (MIC, 20 to ≥50 µg/ml). Activity-guided and chromatographic analysis of butanolic fractions on Sephadex LH-20 column followed by high-performance liquid chromatography, nuclear magnetic resonance analysis and both ultraviolet and thin layer chromatography revealed hydrolysable tannins with a range of activity (MIC, 7.5–27.5 µg/ml or 14–91 µM). Effect of the compounds on fibroblasts did not reveal serious toxicity at moderate concentration but is concentration dependent.
KeywordsMinimum Inhibitory Concentration Ellagic Acid Hydrolysable Tannin Butanolic Fraction Punicalagin
This work was supported by the Japanese Society for the Promotion of Science (JSPS). MNS is a JSPS fellow (ID #: 05166) and wishes to thank Pandey Kishor of the Department of Protozoology, Institute of Tropical Medicine, Nagasaki University and Dr. Haruna Danwanka, Mallam Farouq and Sabo Miri of Abubakar Tafawa Balewa University, Bauchi, Nigeria.
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