Parasitology Research

, Volume 102, Issue 4, pp 697–703 | Cite as

Trypanocidal activity of extracts and compounds from the stem bark of Anogeissus leiocarpus and Terminalia avicennoides

  • Mohammed N. Shuaibu
  • Ponchang T. A. Wuyep
  • Tetsuo Yanagi
  • Kenji Hirayama
  • Akitoyo Ichinose
  • Takashi Tanaka
  • Isao Kouno
Original Paper


The antitrypanosomal activity of methanolic extracts of Anogeissus leiocarpus and Terminalia avicennoides were evaluated in vitro against four strains of Trypanosoma species with minimum inhibitory concentration (MIC) value range of 12.5–50 mg/ml. Successive fractionations of the two plant extracts in water, butanol and ethyl acetate gave a range of activity (MIC, 20 to ≥50 µg/ml). Activity-guided and chromatographic analysis of butanolic fractions on Sephadex LH-20 column followed by high-performance liquid chromatography, nuclear magnetic resonance analysis and both ultraviolet and thin layer chromatography revealed hydrolysable tannins with a range of activity (MIC, 7.5–27.5 µg/ml or 14–91 µM). Effect of the compounds on fibroblasts did not reveal serious toxicity at moderate concentration but is concentration dependent.


Minimum Inhibitory Concentration Ellagic Acid Hydrolysable Tannin Butanolic Fraction Punicalagin 
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This work was supported by the Japanese Society for the Promotion of Science (JSPS). MNS is a JSPS fellow (ID #: 05166) and wishes to thank Pandey Kishor of the Department of Protozoology, Institute of Tropical Medicine, Nagasaki University and Dr. Haruna Danwanka, Mallam Farouq and Sabo Miri of Abubakar Tafawa Balewa University, Bauchi, Nigeria.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Mohammed N. Shuaibu
    • 1
    • 2
  • Ponchang T. A. Wuyep
    • 5
  • Tetsuo Yanagi
    • 3
  • Kenji Hirayama
    • 2
  • Akitoyo Ichinose
    • 4
  • Takashi Tanaka
    • 1
  • Isao Kouno
    • 1
  1. 1.Natural Product Chemistry, Graduate School of Biomedical SciencesNagasaki UniversityNagasaki-shiJapan
  2. 2.Department of Immunogenetics, Institute of Tropical MedicineNagasaki UniversityNagasaki-shiJapan
  3. 3.Animal Research Centre for Tropical Infections, Institute of Tropical MedicineNagasaki UniversityNagasaki-shiJapan
  4. 4.Electron Microscopy Central Laboratory, Institute of Tropical MedicineNagasaki UniversityNagasaki-shiJapan
  5. 5.Department of Biological SciencesAhmadu Bello UniversityZariaNigeria

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