Parasitology Research

, Volume 102, Issue 1, pp 167–169 | Cite as

Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella

  • Jacob Lorenzo-MoralesEmail author
  • Enrique Martínez-Carretero
  • Ninive Batista
  • Jorge Álvarez-Marín
  • Yasmina Bahaya
  • Julia Walochnik
  • Basilio Valladares
Short Communication


A mixed keratitis due to Acanthamoeba and Hartmannella species is reported. The patient was a soft contact lens wearer. Early diagnosis was achieved by polymerase chain reaction and culture. The pathogenic potential of the isolated amoebae was proven using cytotoxicity assays. The reported case underlines the difficulties in identifying a corneal amoebic infection. In our case, the early diagnosis of a mixed infection allowed a proper antiamoebic treatment in an early stage of infection. This may have been the reason of a successful outcome after therapy.


Keratitis Contact Lens Wearer Tobradex Acanthamoeba Isolate Specific Early Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Dr. Lorenzo-Morales was funded by a postdoctoral contract from the Dirección General de Fomento Industrial y Nuevas Tecnologías del Gobierno de Canarias # IDT-TF-06/055. The research was funded by the project RICET (Project No. RD06/0021/0005) of the Programme of Redes Temáticas de Investigación Cooperativa, FIS, by BIOPOLIS-Interreg IIIB (Canarias-Madeira-Açores) project, and a Dirección General de Universidades e Investigación del Gobierno de Canarias project # PI042005/049. The authors are grateful to Ms. Jarmila Kliesciková for her help with the manuscript.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jacob Lorenzo-Morales
    • 1
    Email author
  • Enrique Martínez-Carretero
    • 1
  • Ninive Batista
    • 2
  • Jorge Álvarez-Marín
    • 3
  • Yasmina Bahaya
    • 3
  • Julia Walochnik
    • 4
  • Basilio Valladares
    • 1
  1. 1.University Institute of Tropical Diseases and Public Health of the Canary IslandsUniversity of La LagunaLa LagunaSpain
  2. 2.Microbiology UnitHospital Nuestra Señora de La CandelariaSanta Cruz de TenerifeSpain
  3. 3.Department of OphthalmologyHospital Nuestra Señora de La CandelariaSanta Cruz de TenerifeSpain
  4. 4.Department of Medical ParasitologyMedical University of ViennaViennaAustria

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