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Parasitology Research

, Volume 102, Issue 2, pp 229–232 | Cite as

Sequence characterization of an unusual lysozyme gene expressed in the intestinal tract of the reduviid bug Triatoma infestans (Insecta)

  • C. Balczun
  • E. Knorr
  • H. Topal
  • C. K. Meiser
  • A. H. Kollien
  • G. A. SchaubEmail author
Original Paper

Abstract

Antibacterial proteins like lysozyme are important components of the insect non-specific immune response against bacteria. The complementary deoxyribonucleic acid (cDNA) encoding a new lysozyme from Triatoma infestans, named lysozyme2, has been amplified by polymerase chain reaction and the rapid amplification of cDNA ends technique. The gene is expressed in the small intestine of the insect. The deduced protein sequence shows up to 70% similarity to lysozymes from other species. Furthermore, the protein exhibits significant structural concordance to other insect lysozymes. A striking feature of the lysozyme2 protein is the replacement of the conserved amino acid residues of the active site of classical c-type lysozymes, glutamate and aspartate, by valine and tyrosine.

Keywords

Polymerase Chain Reaction Fragment Putative Signal Peptide Airborne Bacterium Lysozyme Gene Complementary Deoxyribonucleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • C. Balczun
    • 1
  • E. Knorr
    • 1
  • H. Topal
    • 1
  • C. K. Meiser
    • 1
  • A. H. Kollien
    • 1
  • G. A. Schaub
    • 1
    Email author
  1. 1.Zoology/Parasitology GroupRuhr-UniversityBochumGermany

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