Advertisement

Parasitology Research

, Volume 102, Issue 1, pp 103–110 | Cite as

Differences in protein profiles of the isolates of Entamoeba histolytica and E. dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS) ProteinChip assays

  • Asao MakiokaEmail author
  • Masahiro Kumagai
  • Seiki Kobayashi
  • Tsutomu Takeuchi
Original Paper

Abstract

Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) ProteinChip assays with weak cationic exchange chips were used for protein profiling of different isolates of Entamoeba histolytica and E. dispar. When SELDI-TOF MS spectra of cell lysates from E. histolytica strain HM-1:IMSS were compared with those from four other laboratory strains (200:NIH, HK-9, DKB, and SAW755CR) grown under the same culture conditions, different peak patterns of SELDI-TOF MS were observed among these strains, independent of their zymodeme types. Similarly, five Japanese isolates of E. histolytica grown under the same culture conditions revealed different peak patterns among themselves. The SELDI-TOF MS spectra of cell lysates from two isolates of E. dispar strain AS16IR and CYNO 09:TPC showed the presence of peaks specific for E. dispar isolates and the absence of peaks common to E. histolytica isolates. This is not only the first use of SELDI-TOF MS ProteinChip technology for protein profiling of different strains of Entamoeba but also the use for parasitic protozoa. The SELDI-TOF MS spectra show a realistic view of proteins with a biological status of E. histolytica and E. dispar isolates, contributing to show their phenotypic differences of proteins and provide a unique means of distinguishing them.

Keywords

Protein Profile Laboratory Strain Human African Trypanosomiasis Amebiasis Entamoeba Histolytica 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank N Watanabe for his valuable discussion with us, T Obata for SELDI-TOF MS ProteinChip analysis, and T Yamashita for her technical assistance. This work was supported by a Health Science Research Grant for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health, Labor and Welfare of Japan.

References

  1. Ball G, Mian S, Holding F, Allibone RO, Lowe J, Ali S, Li G, McCardle S, Ellis IO, Creaser C, Rees RC (2002) An integrated approach utilizing artificial neural networks and SELDI mass spectrometry for the classification of human tumours and rapid identification of potential biomakers. Bioinformatics 18:395–404PubMedCrossRefGoogle Scholar
  2. Barzaghi D, Isbister JD, Lauer KP, Born TL (2004) Use of surface-enhanced laser desorption/ionization-time of flight to explore bacterial proteomes. Proteomics 4:2624–2628PubMedCrossRefGoogle Scholar
  3. Carlson KA, Ciborowski P, Schellpeper CN, Biskup TM, Shen RF, Luo X, Destache CJ, Gendelman HE (2004) Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report. J Neuroimmunol 147:35–42PubMedCrossRefGoogle Scholar
  4. Carrette O, Demalte I, Scherl A, Yalkinoglu O, Corthals G, Burkhard P, Hochstrasser DF, Sanchez JC (2003) A panel of cerebrospinal fluid potential biomarkers for the diagnosis of Alzheimer’s disease. Proteomics 3:1486–1494PubMedCrossRefGoogle Scholar
  5. Cheng X-J, Tachibana H, Kobayashi S, Kaneda Y, Huang M-Y (1993) Pathogenicity of Entamoeba histolytica isolates from Shanghai, China. Parasitol Res 79:608–610PubMedCrossRefGoogle Scholar
  6. Choe LH, Dutt MJ, Relkin N, Lee KH (2002) Studies of potential cerebrospinal fluid molecular markers for Alzheimer’s disease. Electrophoresis 23:2247–2251PubMedCrossRefGoogle Scholar
  7. Clark CG, Diamond LS (1993) Entamoeba histolytica: a method for isolate identification. Exp Parasitol 77:450–455PubMedCrossRefGoogle Scholar
  8. Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg 72:431–432PubMedCrossRefGoogle Scholar
  9. Engwegen JYMN, Gast MC, Schellens JHM, Beijnen JH (2006) Clinical proteomics: searching for better tumour markers with SELDI-TOF mass spectrometry. Trends Pharmacol Sci 27:251–259PubMedCrossRefGoogle Scholar
  10. Ghosh S, Frisardi M, Ramirez-Avila L, Descoteaux S, Sturm-Ramirez K, Newton-Sanchez OA, Santos-Preciado JI, Ganguly C, Lohia A, Reed S, Samuelson J (2000) Molecular epidemiology of Entamoeba spp.: evidence of a bottleneck (demographic sweep) and transcontinental spread of diploid parasites. J Clin Microbiol 38:3815–3821PubMedGoogle Scholar
  11. Görg A, Weiss W, Dunn MJ (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics 4:3665–3685PubMedCrossRefGoogle Scholar
  12. Haghighi A, Kobayashi S, Takeuchi T, Masuda G, Nozaki T (2002) Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area. J Clin Microbiol 40:4081–4090PubMedCrossRefGoogle Scholar
  13. Haghighi A, Kobayashi S, Takeuchi T, Thammapalerd N, Nozaki T (2003) Geographic diversity among genotypes of Entamoeba histolytica field isolates from a limited geographic area. J Clin Microbiol 41:3748–3756PubMedCrossRefGoogle Scholar
  14. Hayman MW, Przyborski SA (2004) Proteomic identification of biomarkers expressed by human pluripotent stem cells. Biochem Biophys Res Commun 316:918–923PubMedCrossRefGoogle Scholar
  15. Hutchens TW, Yip TT (1993) New desorption strategies for the mass spectrometric analysis of macromolecules. Rapid Commun Mass Spectrom 7:576–580CrossRefGoogle Scholar
  16. Issaq HJ, Veenstra TD, Cornard TP, Felschow D (2002) The SELDI-TOF MS approach to proteomics: protein profiling and biomarker identification. Biochem Biophys Res Commun 292:587–592PubMedCrossRefGoogle Scholar
  17. Kobayashi S, Imai E, Haghighi A, Khalifa SA, Tachibana H, Takeuchi T (2005) Axenic cultivation of Entamoeba dispar in newly desighed yeast extract-iron–gluconic acid–dihydroxyacetone–serum medium. J Parasitol 91:1–4PubMedCrossRefGoogle Scholar
  18. Leitsch D, Radauer C, Paschinger K, Wilson IB, Breiteneder H, Scheiner O, Duchene M (2005) Entamoeba histolytica: analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis. Exp Parasitol 110:191–195PubMedCrossRefGoogle Scholar
  19. Luo X, Carlson KA, Wojna V, Mayo R, Biskup TM, Stoner J, Anderson J, Gendelman HE, Melendez LM (2003) Macrophage proteomic fingerprinting predicts HIV-1-associated cognitive impairment. Neurology 60:1931–1937PubMedGoogle Scholar
  20. Merchant M, Weinberger SR (2000) Recent advancements in surface enhanced laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 21:1164–1177PubMedCrossRefGoogle Scholar
  21. Papadopoulos MC, Abel PM, Stich A, Tarelli E, Bell BA, Planche T, Loosemore A, Saadoun S, Wilkins P, Krishna S (2004) A novel and accurate diagnostic test for human African trypanosomiasis. Lancet 363:1358–1363PubMedCrossRefGoogle Scholar
  22. Sargeaunt PG (1988) Zymodemes of Entamoeba histolytica. In: Radvin JI (ed) Amebiasis: human infection by Entamoeba histolytica. Wiley, New York, pp 370–387Google Scholar
  23. Shah PH, MacFarlane RC, Bhattacharya D, Matese JC, Demeter J, Stroup SE, Singh U (2005) Comparative genomic hybridizations of Entamoeba strains reveal unique genetic fingerprints that correlate with virulence. Eukaryotic Cell 4:504–515PubMedCrossRefGoogle Scholar
  24. Tachibana H, Kobayashi S, Takekoshi M, Ihara S (1991) Distinguishing pathogenic isolates of Entamoeba histolytica by polymerase chain reaction. J Infect Dis 164:825–826PubMedGoogle Scholar
  25. WHO/PAHO/UNESCO (1997) A consultation with experts on amebiasis. Epidemiol Bull 18:13–14Google Scholar
  26. Wulfkuhle JD, McLean KC, Paweletz CP, Sgroi DC, Trock BJ, Steeg PS, Petricoin EF III (2001) New approaches to proteomic analysis of breast cancer. Proteomics 1:1205–1215PubMedCrossRefGoogle Scholar
  27. Yasui Y, Pepe M, Thompson ML, Adam BL, Wright GL Jr, Qu Y, Potter JD, Winget M, Thornquist M, Feng Z (2003) A data-analytic strategy for protein biomarker discovery: profiling of high-dimensional proteomic data for cancer detection. Biostatistics 4:449–463PubMedCrossRefGoogle Scholar
  28. Zaki M, Clark CG (2001) Isolation and characterization of polymorphic DNA from Entamoeba histolytica. J Clin Microbiol 39:897–905PubMedCrossRefGoogle Scholar
  29. Zaki M, Meelu P, Sun W, Clark CG (2002) Simultaneous differentiation and typing of Entamoeba histolytica and Entamoeba dispar. J Clin Microbiol 40:1271–1276PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Asao Makioka
    • 1
    Email author
  • Masahiro Kumagai
    • 1
  • Seiki Kobayashi
    • 2
  • Tsutomu Takeuchi
    • 2
  1. 1.Department of Tropical MedicineJikei University School of MedicineTokyoJapan
  2. 2.Department of Tropical Medicine and ParasitologyKeio University School of MedicineTokyoJapan

Personalised recommendations