Parasitology Research

, Volume 102, Issue 1, pp 103–110 | Cite as

Differences in protein profiles of the isolates of Entamoeba histolytica and E. dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS) ProteinChip assays

  • Asao MakiokaEmail author
  • Masahiro Kumagai
  • Seiki Kobayashi
  • Tsutomu Takeuchi
Original Paper


Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) ProteinChip assays with weak cationic exchange chips were used for protein profiling of different isolates of Entamoeba histolytica and E. dispar. When SELDI-TOF MS spectra of cell lysates from E. histolytica strain HM-1:IMSS were compared with those from four other laboratory strains (200:NIH, HK-9, DKB, and SAW755CR) grown under the same culture conditions, different peak patterns of SELDI-TOF MS were observed among these strains, independent of their zymodeme types. Similarly, five Japanese isolates of E. histolytica grown under the same culture conditions revealed different peak patterns among themselves. The SELDI-TOF MS spectra of cell lysates from two isolates of E. dispar strain AS16IR and CYNO 09:TPC showed the presence of peaks specific for E. dispar isolates and the absence of peaks common to E. histolytica isolates. This is not only the first use of SELDI-TOF MS ProteinChip technology for protein profiling of different strains of Entamoeba but also the use for parasitic protozoa. The SELDI-TOF MS spectra show a realistic view of proteins with a biological status of E. histolytica and E. dispar isolates, contributing to show their phenotypic differences of proteins and provide a unique means of distinguishing them.


Protein Profile Laboratory Strain Human African Trypanosomiasis Amebiasis Entamoeba Histolytica 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank N Watanabe for his valuable discussion with us, T Obata for SELDI-TOF MS ProteinChip analysis, and T Yamashita for her technical assistance. This work was supported by a Health Science Research Grant for Research on Emerging and Re-emerging Infectious Diseases from the Ministry of Health, Labor and Welfare of Japan.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Asao Makioka
    • 1
    Email author
  • Masahiro Kumagai
    • 1
  • Seiki Kobayashi
    • 2
  • Tsutomu Takeuchi
    • 2
  1. 1.Department of Tropical MedicineJikei University School of MedicineTokyoJapan
  2. 2.Department of Tropical Medicine and ParasitologyKeio University School of MedicineTokyoJapan

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