Parasitology Research

, Volume 102, Issue 1, pp 83–90 | Cite as

Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China

  • Lan Hua Li
  • Xiao Ping Zhang
  • Shan Lv
  • Ling Zhang
  • Hisao Yoshikawa
  • Zhiliang Wu
  • Peter Steinmann
  • Jürg Utzinger
  • Xiao Mei Tong
  • Shao Hong Chen
  • Xiao Nong Zhou
Original Paper

Abstract

The prevalence and geographical distribution of the intestinal protozoa Blastocystis in humans across China is unknown, and the relative importance of different subtypes has yet to be investigated. We assessed the community prevalence and relative frequencies of different Blastocystis subtypes in four epidemiological settings in China, i.e., Shanghai municipality, Yongjia county (Zhejiang province), Eryuan county, and Menghai county (both Yunnan province). Blastocystis infection was detected with the culture method, and the subtype was identified with polymerase chain reaction using a set of subtype-specific primers. The prevalence at the four study settings was 1.9, 5.9, 18.4, and 32.6%, respectively. People aged greater than or equal to 60 years had a higher prevalence in the former two settings, Shanghai and Yongjia, whereas the highest infection rate was found among individuals aged 10–17 years in the latter two settings, Eryuan and Menghai. A higher prevalence was found in men in the former two settings but in women in the latter two settings. Five different Blastocystis subtypes were identified from the 192 isolates. Subtype 3 was the predominant type, followed by subtype 1. In conclusion, the epidemiology of Blastocystis varies across China.

References

  1. Banister J, Zhang XB (2005) China, economic development and mortality decline. World Dev 33:21–41CrossRefGoogle Scholar
  2. Bohm-Gloning B, Knobloch J, Walderich B (1997) Five subgroups of Blastocystis hominis from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop Med Int Health 2:771–778PubMedCrossRefGoogle Scholar
  3. Brumpt E (1912) Blastocystis hominis n.sp. et formes voisines. Bull Soc Pathol Exot 5:725–730Google Scholar
  4. Clark CG (1997) Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 87:79–83PubMedCrossRefGoogle Scholar
  5. El-Shazly AM, Abdel-Magied AA, El-Beshbishi SN, El-Nahas HA, Fouad MA, Monib MS (2005) Blastocystis hominis among symptomatic and asymptomatic individuals in Talkha Center, Dakahlia Governorate, Egypt. J Egypt Soc Parasitol 35:653–666PubMedGoogle Scholar
  6. Ertug S, Karakas S, Okyay P, Ergin F, Oncu S (2006) The effect of Blastocystis hominis on the growth status of children. Med Sci Monit 13:CR40–CR43PubMedGoogle Scholar
  7. Ho LC, Singh M, Suresh K, Ng GC, Yap EH (1994) A study of the karyotypic patterns of Blastocystis hominis by pulsed-field gradient electrophoresis. Parasitol Res 80:620–622PubMedCrossRefGoogle Scholar
  8. Hunter PR, Hughes S, Woodhouse S, Syed Q, Verlander NQ, Chalmers RM, Morgan K, Nichols G, Beeching N, Osborn K (2004) Sporadic cryptosporidiosis case–control study with genotyping. Emerg Infect Dis 10:1241–1249PubMedGoogle Scholar
  9. Init I, Mak JW, Lokman Hakim S, Yong HS (1999) Strain differences in Blastocystis isolates as detected by a single set of polymerase chain reaction primers. Parasitol Res 85:131–134PubMedCrossRefGoogle Scholar
  10. Jin QX, Yu KM, Tang LF, Tian CL, Lu ZC (2005) Investigation of infectious status of Blastocystis hominis in 1354 outpatients. China Trop Med 5:1469–1471 (in Chinese)Google Scholar
  11. Karanis P, Kourenti C, Smith H (2007) Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J Water Health 5:1–38PubMedCrossRefGoogle Scholar
  12. Lai KP (1992) Intestinal protozoan infections in Malaysia. Southeast Asian J Trop Med Public Health 23:578–586PubMedGoogle Scholar
  13. Li LH, Zhou XN, Du ZW, Wang XZ, Wang LB, Jiang JY, Yoshikawa H, Steinmann P, Utzinger J, Wu Z, Chen JX, Chen SH, Zhang L (2007) Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. Parasitol Int 56:281–286PubMedCrossRefGoogle Scholar
  14. Liu CH, Han JJ, Liu CY, Wang ZY (1997) Survey on human parasites infection in Sichuan province. Sichuan J Zool 16:105–108 (in Chinese)Google Scholar
  15. Muller M, Sanchez RM, Suswillo RR (1989) Evaluation of a sanitation programme using eggs of Ascaris lumbricoides in household yard soils as indicators. J Trop Med Hyg 92:10–16PubMedGoogle Scholar
  16. Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E (2005) Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 43:348–355PubMedCrossRefGoogle Scholar
  17. Schulz S, Kroeger A (1992) Soil contamination with Ascaris lumbricoides eggs as an indicator of environmental hygiene in urban areas of north-east Brazil. J Trop Med Hyg 95:95–103PubMedGoogle Scholar
  18. Scicluna SM, Tawari B, Clark CG (2006) DNA barcoding of Blastocystis. Protist 157:77–85PubMedCrossRefGoogle Scholar
  19. Steinmann P, Zhou XN, Li YL, Li HJ, Chen SR, Yang Z, Fan W, Jia TW, Li LH, Vounatsou P, Utzinger J (2007a) Helminth infections and risk factor analysis among residents in Eryuan county, Yunnan province, China. Acta Trop 104 (in press)Google Scholar
  20. Steinmann P, Zhou XN, Du ZW, Jiang JY, Wang LB, Wang XZ, Li LH, Marti H, Utzinger J (2007b) Occurrence of Strongyloides stercoralis in Yunnan province, China and comparison of diagnostic methods. PLoS Negl Trop Dis 1:e75, doi:10.1371/journal.pntd.0000075 Google Scholar
  21. Stensvold CR, Brillowska-Dabrowska A, Nielsen HV, Arendrup MC (2006) Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J Parasitol 92:1081–1087PubMedCrossRefGoogle Scholar
  22. Stensvold CR, Suresh GK, Tan KSW, Thompson RCA, Traub RJ, Viscogliosi E, Yoshikawa H, Clark CG (2007) Terminology for Blastocystis subtypes—a consensus. Trends Parasitol 23:93–96PubMedCrossRefGoogle Scholar
  23. Stenzel DJ, Boreham PFL (1996) Blastocystis hominis revisited. Clin Microbiol Rev 9:563–584PubMedGoogle Scholar
  24. Su YP, He LJ, Shang LY, Li C (1995) Research status of Blastocystis hominis in China. Henan J Prev Med 6:361–363 (in Chinese)Google Scholar
  25. Suresh K, Smith H (2004) Comparison of methods for detecting Blastocystis hominis. Eur J Clin Microbiol Infect Dis 23:509–511PubMedCrossRefGoogle Scholar
  26. Tan KSW, Singh M, Yap EH (2002) Recent advances in Blastocystis hominis research: hot spots in terra incognita. Int J Parasitol 32:789–804PubMedCrossRefGoogle Scholar
  27. Termmathurapoj S, Leelayoova S, Aimpun P, Thathaisong U, Nimmanon T, Taamasri P, Mungthin M (2004) The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol Res 93:445–447PubMedCrossRefGoogle Scholar
  28. Upcroft JA, Dunn LA, Dommett LS, Healey A, Upcroft P, Boreham PF (1989) Chromosomes of Blastocystis hominis. Int J Parasitol 19:879–883PubMedCrossRefGoogle Scholar
  29. Wang J, Li CP (2002) Epidemiology investigation of Blastocystis hominis in Huainan areas. J Fourth Military Med Univers 23:1480–1482 (in Chinese)Google Scholar
  30. Wang YK, Yu H, Yang JL, Yang HM (1994) A survey of human intestinal protozoa in Yunnan. Chin J Parasitol Parasit Dis 12:89–92 (in Chinese)Google Scholar
  31. Woolhouse MEJ (1998) Patterns in parasite epidemiology: the peak shift. Parasitol Today 14:428–434PubMedCrossRefGoogle Scholar
  32. Xu LQ, Yu SH, Xu SH (2000) Distribution and pathogenic impact of human parasites in China. People's Medical, Beijing China (in Chinese)Google Scholar
  33. Yan YM, Su SL, Lai RY, Liao H, Ye JH, Li XB, Luo XT, Chen GF (2006) Genetic variability of Blastocystis hominis isolates in China. Parasitol Res 99:597–601PubMedCrossRefGoogle Scholar
  34. Yoshikawa H, Nagano I, Wu Z, Yap EH, Singh M, Takahashi Y (1998) Genomic polymorphism among Blastocystis hominis strains and development of subtype-specific diagnostic primers. Mol Cell Probes 12:153–159PubMedCrossRefGoogle Scholar
  35. Yoshikawa H, Abe N, Iwasawa M, Kitano S, Nagano I, Wu Z, Takahashi Y (2000) Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. J Clin Microbiol 38:1324–1330PubMedGoogle Scholar
  36. Yoshikawa H, Abe N, Wu Z (2003) Genomic polymorphism among Blastocystis isolates and development of PCR-based identification of zoonotic isolates. J Eukaryot Microbiol 50(Suppl):710–711PubMedCrossRefGoogle Scholar
  37. Yoshikawa H, Morimoto K, Wu Z, Singh M, Hashimoto T (2004a) Problems in speciation in the genus Blastocystis. Trends Parasitol 20:251–255PubMedCrossRefGoogle Scholar
  38. Yoshikawa H, Wu Z, Kimata I, Iseki M, Ali IK, Hossain MB, Zaman V, Haque R, Takahashi Y (2004b) Polymerase chain reaction-based subtype classification among human Blastocystis hominis populations isolated from different countries. Parasitol Res 92:22–29PubMedCrossRefGoogle Scholar
  39. Zierdt CH (1991) Blastocystis hominis—past and future. Clin Microbiol Rev 4:61–79PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lan Hua Li
    • 1
    • 2
  • Xiao Ping Zhang
    • 3
  • Shan Lv
    • 1
  • Ling Zhang
    • 1
  • Hisao Yoshikawa
    • 4
  • Zhiliang Wu
    • 5
  • Peter Steinmann
    • 6
  • Jürg Utzinger
    • 6
  • Xiao Mei Tong
    • 1
  • Shao Hong Chen
    • 1
  • Xiao Nong Zhou
    • 1
  1. 1.National Institute of Parasitic DiseasesChinese Center for Disease Control and PreventionShanghaiPeople’s Republic of China
  2. 2.Department of Preventive MedicineWeifang Medical UniversityWeifangPeople’s Republic of China
  3. 3.Center for Disease Control and Prevention of ShanghaiShanghaiPeople’s Republic of China
  4. 4.Department of Biological SciencesNara Women’s UniversityNaraJapan
  5. 5.Department of ParasitologyGifu University Graduate School of MedicineGifuJapan
  6. 6.Department of Public Health and EpidemiologySwiss Tropical InstituteBaselSwitzerland

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