Parasitology Research

, Volume 101, Issue 6, pp 1657–1661 | Cite as

Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica

  • K. MatanovićEmail author
  • K. Severin
  • F. Martinković
  • M. Šimpraga
  • Z. Janicki
  • J. Barišić
Original Paper


A naturally occurring outbreak of fasciolosis in a group of 20 Merinolandschaf (German Merino) sheep was studied. Hematological and blood biochemical values in sheep spontaneously infected with liver fluke Fasciola hepatica were compared with equivalent values in 20 parasite-free sheep from organically farmed flock. Investigated animals were kept in outdoor system, on pastures covered with swamps, which remain flooded after rainy season. Significantly lower red blood cell (RBC) count, lymphocytes, hemoglobin (Hb), packed cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine, and albumin were recorded in sheep from the infected herd, whereas white blood cell (WBC) count, eosinophil, segmented and band neutrophil count, mean corpuscular volume (MCV), gamma-glutamyl transferase (GGT), concentrations of glucose, and globulins were significantly higher than in the parasite free herd. No significant correlation between the investigated blood parameters and the number of F. hepatica eggs in the feces was detected. This study shows that hematological and biochemical values can be useful in early diagnosis and prognosis of sheep fasciolosis.


Packed Cell Volume Mean Corpuscular Volume Mean Corpuscular Hemoglobin Mean Corpuscular Hemoglobin Concentration Gastrointestinal Nematode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the projects TP3083 and 0053301 (Ministry of Science, Education and Sport, Croatia) and complies with the current laws for research on animals in Croatia.


  1. Akinbamijo OO, Lahlou-Kassi A, Tembely S (1996) Effect of experimental fascioliasis on feed intake, nitrogen retention and body weight changes in open and pregnant Menz sheep. Small Rumin Res 20:163–169CrossRefGoogle Scholar
  2. Anderson PH, Berrett S, Brush PJ, Hebert CN, Parfitt JW, Patterson DS (1977) Biochemical indicators of liver injury in calves with experimental fascioliasis. Vet Rec 100:43–45PubMedGoogle Scholar
  3. Boray JC (1985) Flukes of domestic animals. In: SM Gaafar, WE Howard, RE Marsh (eds) Parasites, pests and predators. Elsevier, Amsterdam, pp 179–218Google Scholar
  4. Cabaret J, Bouilhol M, Mage C (2002) Managing helminths of ruminants in organic farming. Vet Res 33:625–640PubMedCrossRefGoogle Scholar
  5. Ferre I, Lopez P, Gonzalo-Orden M, Julian MD, Rojovazquez FA (1995) The effects of subclinical fasciolosis on hepatic secretory function in sheep. Parasitol Res 81:127–131PubMedCrossRefGoogle Scholar
  6. Gonzalo-Orden M, Millàn L, Àlvarez M, Sànchez-Campos S, Jiménez R, Gonzalez-Gallego J, Tuńón MJ (2003) Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings. Parasitol Res 90:359–364PubMedCrossRefGoogle Scholar
  7. Haroun EM, A/Gadir H, Gameel AA (1986) Studies on naturally occurring ovine fascioliasis in the Sudan. J Helminthol 60:47–53PubMedCrossRefGoogle Scholar
  8. Hawkins CD (1984) The use of haemoglobin, packed-cell volume and serum sorbitol dehydrogenase as indicators of the development of fascioliasis in sheep. Vet Parasitol 15:125–133PubMedCrossRefGoogle Scholar
  9. Kaneko JJ, Harvey JW, Bruss ML (1997) Clinical Biochemistry of Domestic Animals, 5th edn. Academic, San Diego, pp 890–894Google Scholar
  10. Kramer JW (2000) Normal hematology of cattle, sheep and goats. In: Feldman BF, Zinkl JG, Jain NC (eds) Schalm’s veterinary hematology, 5th edn. Lippincott Williams & Wilkins, Philadelphia, pp 1075–1084Google Scholar
  11. Mulcahy G, O’Connor F, Clery D, Hogan SF, Dowd AJ, Andrews SJ, Dalton JP (1999) Immune responses of cattle to experimental anti-Fasciola hepatica vaccines. Res Vet Sci 67:27–33PubMedCrossRefGoogle Scholar
  12. Paz-Silva A, Sanchez-Andrade R, Suarez JL, Pedreira R, Arias M, Lopez C, Panadero R, Diaz P, Diez-Banos P, Morrondo P (2003) Prevalence of natural ovine fasciolosis shown by demonstrating the presence of serum circulating antigens. Parasitol Res 91:328–331PubMedCrossRefGoogle Scholar
  13. Price TA, Tuazon CA, Simon GL (1993) Fascioliasis: case reports and review. Clin Infect Dis 17:426–430PubMedGoogle Scholar
  14. Scott PR, Sargison ND, Macrae A, Rhind SR (2005) An outbreak of subacute fasciolosis in Soay sheep: ultrasonographic biochemical and histological studies. Vet J 170:325–331PubMedCrossRefGoogle Scholar
  15. Sinclair KB (1967) The anemia in fascioliasis: experiments with Cr51 labeled red cells. Vet Rec 80:363PubMedGoogle Scholar
  16. Swarup D, Upadhyay DS, Pachauri SP (1986) Some biochemical indices in naturally occurring fascioliasis in goats. Res Vet Sci 40:276–277PubMedGoogle Scholar
  17. Sykes AR, Coop RL, Rushton B (1980) Chronic subclinical fascioliasis in sheep: effects on food intake, food utilisation and blood constituents. Res Vet Sci 28:63–70PubMedGoogle Scholar
  18. Zhang ZWY, Moreau E, Hope JC, Howard CJ, Huang WY, Chauvin A (2005) Fasciola hepatica and Fasciola gigantica: comparison of cellular response to experimental infection in sheep. Exp Parasitol 111:154–59PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • K. Matanović
    • 1
    Email author
  • K. Severin
    • 2
  • F. Martinković
    • 3
  • M. Šimpraga
    • 1
  • Z. Janicki
    • 2
  • J. Barišić
    • 2
  1. 1.Department of Physiology and Radiobiology, Faculty of Veterinary MedicineUniversity of ZagrebZagrebCroatia
  2. 2.Department of Game Biology, Pathology and Breeding, Faculty of Veterinary MedicineUniversity of ZagrebZagrebCroatia
  3. 3.Department of Parasitology and Parasitic Diseases with Clinic, Faculty of Veterinary MedicineUniversity of ZagrebZagrebCroatia

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