Advertisement

Parasitology Research

, Volume 101, Issue 5, pp 1295–1301 | Cite as

Ultrastructural study of the spermatozoon of Nicolla testiobliquum (Digenea, Opecoelidae) parasite of brown trout Salmo trutta (Pisces, Teleostei)

  • Yann QuilichiniEmail author
  • Josephine Foata
  • Bernard Marchand
Original Paper

Abstract

The present paper describes the spermatozoon of Nicolla testiobliquum (Digenea, Opecoelidae), an intestinal parasite of brown trout Salmo trutta, studied by transmission electron microscopy. The mature spermatozoon possess two axonemes of 9 + “1” pattern, two mitochondria, a nucleus, external ornamentation of the plasma membrane, spine-like bodies and cortical microtubules. A comparative study between N. testiobliquum and Nicolla wisniewskii shows mainly two different dispositions of the two mitochondria in these two species of a same genera. Moreover, the comparison of each of these spermatozoon features with others digeneans, in general, and other Opecoelidae in particular, is also presented. It appears that the organisation of the posterior part of the spermatozoon, the disposition of cortical microtubules and external ornamentation associated with spine-like bodies could be interesting elements for phylogeny.

Keywords

Seminal Vesicle Anterior Part Posterior Part Brown Trout Propylene Oxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Agostini S, Miquel J, Ndiaye P, Marchand B (2005) Dicrocoelium hospes Looss, 1907 (Digenea, Dicrocoeliidae): spermiogenesis, mature spermatozoon and ultrastructural comparative study. Parasitol Res 96:38–48PubMedCrossRefGoogle Scholar
  2. Bâ CT, Marchand B (1995) Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda. In: Jamieson BGM, Ausio J, Justine JL (eds) Advances in spermatozoal phylogeny and taxonomy. Mém Mus Natl Hist Nat Paris, pp 87–95Google Scholar
  3. Brooks DR (1989) A summary of the database pertaining to the phylogeny of the major groups of the parasitic platyhelminths, with a revised classification. Can J Zool 67:714–720CrossRefGoogle Scholar
  4. Burton PR (1972) Fine structure of the reproductive system of a frog lung fluke. III. The spermatozoon and its differentiation. J Parasitol 58:68–83PubMedCrossRefGoogle Scholar
  5. Ehlers U (1984) Phylogenetisches system der Platyhelminthes. Verh Natwiss Ver Hambg (NF) 27:291–294Google Scholar
  6. Ehlers U (1986) Comments on a phylogenetic system of the Platyhelminthes. Hydrobiologia 132:1–12CrossRefGoogle Scholar
  7. Erwin BE, Halton DW (1983) Fine structural observations on spermatogenesis in a progenetic trematode, Bucephaloides gracilescens. Int J Parasitol 13:413–426CrossRefGoogle Scholar
  8. Euzet L, Swiderski Z, Mokhtar-Maamouri F (1981) Ultrastructure comparée du spermatozoïde des cestodes. Relations avec la phylogénèse. Ann Parasitol Hum Comp 56:247–259PubMedGoogle Scholar
  9. Fujino T, Ishii Y, Mori T (1977) Ultrastructural studies on the spermatozoa and spermatogenesis in Paragonimus and Eurytrema (Trematoda : Digenea). Jpn J Parasitol 26:240–255Google Scholar
  10. Gracenea M, Ferrer JR, Gonzalez-Moreno O, Trullols M (1997) Ultrastructural study of spermatogenesis and the spermatozoon in Postorchigenes gymnesicus (Trematoda, Lecithodendriidae). J Morphol 234:223–232CrossRefGoogle Scholar
  11. Jamieson BGM, Daddow LM (1982) The ultrastructure of the spermatozoon of Neochasmus sp. (Cryptogonimidae, Digenea, Trematoda) and its phylogenetic significance. Int J Parasitol 12:547–559CrossRefGoogle Scholar
  12. Jones A, Bray RA, Gibson DI (2005) Keys to the Trematoda. CAB International, WallingfordGoogle Scholar
  13. Justine JL (1991) Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa. Can J Zool 69:1421–1440Google Scholar
  14. Justine JL (1995) Spermatozoal ultrastructure and phylogeny in the parasitic Platyhelminthes. Mém Mus Natl Hist Nat 166:55–86Google Scholar
  15. Justine JL (1997) La classification générale des Platyhelminthes parasites: changements récents et utilisation des caractères ultrastructuraux, en particulier des spermatozoïdes. Bull Soc Zool Fr 122:269–277Google Scholar
  16. Justine JL, Mattei X (1982a) Etude ultrastructurale de la spermiogenèse et du spermatozoïde d’un plathelminthe: Gonapodasmius (Trematoda : Didymozoidae). J Ultrastruct Res 79:350–365PubMedCrossRefGoogle Scholar
  17. Justine JL, Mattei X (1982b) Réinvestigation de l’ultrastructure du spermatozoïde d’Haematoloechus (Trematoda : Haematoloechidae). J Ultrastruct Res 81:322–332PubMedCrossRefGoogle Scholar
  18. Justine JL, Mattei X (1983) A spermatozoon with two 9 + 0 axonemes in a parasitic flatworm, Didymozoon (Digenea : Didymozoidea). J Submicrosc Cytol 15:1101–1105Google Scholar
  19. Justine JL, Lambert A, Mattei X (1985) Spermatozoon ultrastructure and phylogenetic relationships in the Monogenea (Platyhelminthes). Int J Parasitol 15:601–608PubMedCrossRefGoogle Scholar
  20. Levron C, Ternengo S, Marchand B (2003) Ultrastructure of spermiogenesis and the spermatozoon of Helicometra fasciata (Digenea, Opecoelidae), a parasite of Labrus merula (Pisces, Teleostei). Acta Parasitol 48:255–264Google Scholar
  21. Levron C, Ternengo S, Marchand B (2004a) Spermiogenesis and sperm ultrastructure of Diphterostomum brusinae (Digenea, Zoogonidae), a parasite of Diplodus annularis (Pisces, Teleostei). Parasitol Res 94:147–154PubMedGoogle Scholar
  22. Levron C, Ternengo S, Marchand B (2004b) Spermiogenesis and sperm ultrastructure of Poracanthium furcatum (Digenae, Opecoelidae), a parasite of Mullus surmuletus (Pisces, Teleostei). Acta Parasitol 49:190–200Google Scholar
  23. Levron C, Ternengo S, Marchand B (2004c) Ultrastructure of spermiogenesis and the spermatozoon of Monorchis parvus (Digenae, Monorchiidae), a parasite of Diplodus annularis (Pisces, Teleostei). Parasitol Res 93:102–110PubMedCrossRefGoogle Scholar
  24. Miquel J, Nourrisson C, Marchand B (2000) Ultrastructure of spermiogenesis and the spermatozoon of Opecoeloides furcatus (Trematoda, Digenea, Opecoelidae), a parasite of Mullus barbatus (Pisces, Teleostei). Parasitol Res 86:301–310PubMedCrossRefGoogle Scholar
  25. Miquel J, Fournier-Chambrillon C, Fournier P, Torres J (2006) Spermiogenesis and spermatozoon ultrastructure of the cranial digenean Troglotrema acutum (Leuckart, 1842). J Parasitol 92:441–453PubMedCrossRefGoogle Scholar
  26. Ndiaye PI, Miquel J, Ba CT, Marchand B (2001) Desarollo espermatico en Fasciola gigantica Cobbold, 1856 (Digenea, Fasciolidae): estudio ultraestructural. Acta Parasitol Port 8:17Google Scholar
  27. Ndiaye PN, Miquel J, Ba CT, Feliu C, Marchand B (2002) Spermiogenesis and sperm ultrastructure of Scaphiostomum palaearcticum Mas-Coma, Esteban et Valero, 1986 (Trematoda, Digenea, Brachylaimidae). Acta Parasitol 47:259–271Google Scholar
  28. Ndiaye PI, Miquel J, Fons R, Marchand B (2003a) Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica Linnaeus, 1758 (Digenea, Fasciolidae): Transmission and scanning electron microscopy, and tubulin immunocytochemistry. Acta Parasitol 48:182–194Google Scholar
  29. Ndiaye PI, Miquel J, Marchand B (2003b) Ultrastructure of spermiogenesis and spermatozoa of Notocotylus neyrai Gonzalez Castro, 1945 (Digenea, Notocotylidae) intestinal parasite of Microtus agrestis (Rodents: Arvicolidae) in Spain. Invertebr Reprod Dev 42:105–115Google Scholar
  30. Ndiaye PI, Miquel J, Ba CT, Marchand B (2004) Ultrastructure of spermiogenesis and the spermatozoon of the liver fluke Fasciola gigantica Cobbold, 1856 (Digenea, Fasciolidae), a parasite of cattle in Sénégal. J Parasitol 90:30–40PubMedCrossRefGoogle Scholar
  31. Orido Y (1988) Ultrastructure of spermatozoa of the lung fluke, Paragonimus ohirai (Trematoda:Troglotrematidae), in the seminal receptacle. J Morphol 196:333–343PubMedCrossRefGoogle Scholar
  32. Quilichini Y, Foata J, Orsini A, Marchand B (2007a) Spermiogenesis and spermatozoon ultrastructure of Nicolla wisniewskii (Digenea, Opecoelidae), an intestinal parasite of brown trout Salmo trutta (Pisces, Teleostei). J Parasitol 93:469–478PubMedCrossRefGoogle Scholar
  33. Quilichini Y, Foata J, Orsini A, Marchand B (2007b) Ultrastructural study of spermiogenesis and the spermatozoon of Crepidostomum metoecus (Digenea: Allocreadiidae), a parasite of Salmo trutta (Pisces: Teleostei). J Parasitol 93:458–468PubMedCrossRefGoogle Scholar
  34. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212PubMedCrossRefGoogle Scholar
  35. Rohde K (1990) Phylogeny of Platyhelminthes, with special reference to parasitic groups. Int J Parasitol 20:979–1007PubMedCrossRefGoogle Scholar
  36. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43PubMedCrossRefGoogle Scholar
  37. Stitt AW, Fairweather I (1990) Spermatogenesis and the fine structure of the mature spermatozoon of the liver fluke, Fasciola hepatica (Trematoda: Digenea). Parasitology 101:395–407PubMedGoogle Scholar
  38. Tang J, Wang W, Wang G (1998) Studies on ultrastructures of spermatogenesis and sperm in Pseudorhipidocotyle elpichthys. Acta Hydrobiol Sin 22:168–174Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yann Quilichini
    • 1
    Email author
  • Josephine Foata
    • 1
  • Bernard Marchand
    • 1
  1. 1.Laboratoire Parasites et Ecosystèmes méditerranéens, Faculté des Sciences et TechniquesUniversité de CorseCorteFrance

Personalised recommendations