Parasitology Research

, Volume 101, Issue 4, pp 1007–1013 | Cite as

Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis

  • Heliana B. Oliveira
  • Gleyce A. Machado
  • Dagmar D. Cabral
  • Julia M. Costa-Cruz
Original Paper


Serological tests are an important tool for the diagnosis of neurocysticercosis (NCC), the disease caused by Taenia solium metacestodes. The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in the immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Western blotting (WB) tests compared with the metacestodes antigen of T. solium in serum samples. The samples were obtained from 130 individuals: 20 from patients with definitive NCC, Group 1; 18 from individuals infected by Taenia sp., Group 2; 40 from individuals infected by various parasites, Group 3; and 40 from healthy individuals, Group 4. The sensitivity of IFAT, ELISA, and WB using antigen obtained from T. solium applied to the patients of Group 1 yielded results of 85, 95, and 95%, respectively, for the three tests. When the tests were conducted using T. saginata metacestodes, results were 75, 80, and 85%, respectively. The specificity of IFAT, ELISA, and WB using antigen obtained from T. solium yielded results of 94.9, 88.8, and 93.9%. When the tests were conducted using T. saginata metacestodes, results were 95.9, 88.8, and 93.6%, respectively. No statistical differences for sensitivity or specificity among the antigens were found. In conclusion, the results indicated that T. saginata metacestodes can be used as an alternative antigen for NCC diagnosis.



This study was supported by the Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Coordenação e Aperfeiçoamento Pessoal de Nível Superior (CAPES), and the Federal University of Uberlândia (UFU), Brazil.

The experiments described comply with the current laws of Brazil, and approval was received from the Ethics Committee of the Federal University of Uberlândia, Minas Gerais.


  1. Bragazza LM, Vaz AJ, Passos ADC, Takayanagui OM, Nakamura PM, Espíndolo NM, Pardini A, Bueno EC (2002) Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens. Rev Inst Med Trop São Paulo 44:7–12PubMedGoogle Scholar
  2. Chung JY, Yun DH, Eom KS, Kang SY, Kong Y, Cho SY (2002) Taenia solium: identification of specific antibody binding regions of metacestode 10-kDa protein. Exp Parasitol 100:87–94PubMedCrossRefGoogle Scholar
  3. Costa JM, Ferreira AW, Makino MM, Camargo M (1982) Spinal fluid immunoenzymatic assay (ELISA) for neurocysticercosis. Rev Inst Med Trop São Paulo 24:337–341PubMedGoogle Scholar
  4. DeGiorgio C, Pietsch-Escueta S, Tsang V, Corral-Leyva G, Ng L, Medina MT, Astudillo S, Padilla N, Leyva P, Martinez L, Noh J, Levine M, del Villasenor R, Sorvillo F (2005) Sero-prevalence of Taenia solium cysticercosis and Taenia solium taeniasis in California, USA. Acta Neurol Scand 111:84–88PubMedCrossRefGoogle Scholar
  5. Del Brutto OH, Wadia NH, Dumas M, Cruz M, Tsang VCW, Nash TE, Schantz PM (1996) Proposal of diagnostic criteria for human cysticercosis and neurocysticercosis. J Neurol Sci 142:1–6PubMedCrossRefGoogle Scholar
  6. Fleury A, Morales J, Bobes RJ, Dumas M, Yánez O, Piña J, Carrillo-Mezo R, Martínez JJ, Fragoso G, Dessein A, Larralde C, Sciutto E (2006) An epidemiological study of familial neurocysticercosis in an endemic Mexican community. Trans R Soc Trop Med Hyg 100:551–558PubMedCrossRefGoogle Scholar
  7. Flisser A, Sarti E, Lightowlers M, Schantz P (2003) Neurocysticercosis: regional status, epidemiology, impact and control measures in the Americas. Acta Trop 87:43–51PubMedGoogle Scholar
  8. Gekeler F, Eichenlaub S, Mendoza EG, Sotelo J, Hoelscher M, Löscher T (2002) Sensivity and specificity of ELISA and immunoblot for diagnosing neurocysticercosis. Eur J Clin Microbiol Infect Dis 21:227–229PubMedCrossRefGoogle Scholar
  9. Hamed SA, El-Metaal HE (2007) Unusual presentations of neurocysticercosis. Acta Neurol Scand 115:192–198PubMedCrossRefGoogle Scholar
  10. Hawk MW, Shahlaie K, Kim KD, Theis JH (2005) Neurocysticercosis: a review. Surg Neurol 63:123–132PubMedCrossRefGoogle Scholar
  11. Ishida MMI, Rubinsky-Elefant G, Ferreira AW, Hoshino-Shimizu S, Vaz AJ (2003) Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 89:73–84PubMedCrossRefGoogle Scholar
  12. Ito A, Nakao M, Wandra T (2003) Human taeniasis and cysticercosis in Asia. Lancet 362:1918–1920PubMedCrossRefGoogle Scholar
  13. Kong Y, Kang SY, Cho SY, Min DY (1989) Cross-reacting and specific antigens components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium. Kisengchunghak Chapji 27:131–139Google Scholar
  14. Kumar GR (2004) Diagnostic criteria for neurocysticercosis: some modifications are needed for Indian patients. Neurol India 52:171–177Google Scholar
  15. Lowry VH, Rosebrouch NJ, Farr AL, Randal RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 29:265–275Google Scholar
  16. Machado ER, Costa-Cruz JM (1998) Strongyloides stercoralis and other enteroparasites in children at Uberlândia city, state of Minas Gerais, Brazil. Mem Inst Oswaldo Cruz 93:161–164PubMedGoogle Scholar
  17. Machado A, Camargo ME, Hoshino S (1973) Reação de imunofluorescência para a cisticercose com partículas de Cysticercus cellulosae fixadas à lâminas de microscopia. Rev Soc Bras Med Trop 7:181–183Google Scholar
  18. Montenegro T, Gilman R, Castillo R, Tsang V, Branot J, Guevara A, Sanabria H, Verastegui M, Sterling C, Miranda E (1994) The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus and Hymenolepis nana. Rev Inst Med Trop São Paulo 36:327–334PubMedGoogle Scholar
  19. Morakote N, Nawacharoen W, Sukonthasun K, Thammasonthi W, Khamboonruang C (1992) Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis. Southeast Asian J Trop Med Public Health 23:77–81PubMedGoogle Scholar
  20. Mukaratirwa S, Kassuku AA, Willingham AL, Murrell KD (2003) Background to the international action planning workshop on Taenia solium cysticercosis/taeniosis with special focus on Eastern and Southern Africa. Acta Trop 87:3–5PubMedGoogle Scholar
  21. Oliveira HB, Rodrigues RM, Barcelos ISC, Silva LP, Costa-Cruz JM (2006) Anti-Taenia solium metacestodes IgG antibodies in serum samples from inhabitants of a central-western region of Brazil. Rev Inst Med Trop São Paulo 48:49–52PubMedGoogle Scholar
  22. Roca C, Gascón J, Font B, Pujol T, Valls ME (2003) Neurocysticercosis and population movements: analysis of 23 imported cases in Spain. Eur J Clin Microbiol Infect Dis 22:382–384PubMedCrossRefGoogle Scholar
  23. Sako Y, Nakao M, Nakaya K, Yamasaki H, Ito A (2006) Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis. Parasitol Int 55:69–73CrossRefGoogle Scholar
  24. Schantz PM (2006) Progress in diagnosis, treatment and elimination of echinococcosis and cysticercosis. Parasitol Int 55:7–13CrossRefGoogle Scholar
  25. Shiguekawa KYM, Mineo JR, Moura LP, Costa-Cruz JM (2000) ELISA and Western blotting tests in the detection of IgG antibodies to Taenia solium metacestodes in serum samples in human neurocysticercosis. Trop Med Int Health 5:443–449PubMedCrossRefGoogle Scholar
  26. Sikasunge CS, Phiri IK, Phiri AM, Dorny P, Siziya S, Willingham AL III (2007) Risk factors associated with porcine cysticercosis in selected districts of Eastern and Southern provinces of Zambia. Vet Parasitol 143:59–66PubMedCrossRefGoogle Scholar
  27. Silveira-Lacerda EP, Machado ER, Arantes SCF, Costa-Cruz JM (2002) Anti-Taenia solium metacestodes antibodies in serum from blood donors from four cities of Triângulo Mineiro area, Minas Gerais, Brazil, 1995. Rev Inst Med Trop São Paulo 44:229–231Google Scholar
  28. Takayanagui OM, Odashima NS (2006) Clinical aspects of neurocysticercosis. Parasitol Int 55(Suppl 1):111–115CrossRefGoogle Scholar
  29. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedCrossRefGoogle Scholar
  30. Zheng Y, Cai X, Luo X, Hu Z, Jing Z (2007) Characterization of a new gene (SLC10) with a spliced leader from Taenia solium. Vet J (in press). DOI  10.1016/j.tvjl.2006.11.020

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Heliana B. Oliveira
    • 1
  • Gleyce A. Machado
    • 1
  • Dagmar D. Cabral
    • 1
  • Julia M. Costa-Cruz
    • 1
  1. 1.Laboratório de Parasitologia, Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências BiomédicasUniversidade Federal de UberlândiaUberlândiaBrazil

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