Parasitology Research

, Volume 101, Issue 4, pp 1135–1139 | Cite as

Molecular characterization of Echinococcus granulosus in sheep and goats of Peloponnesus, Greece

  • A. VarcasiaEmail author
  • S. Canu
  • A. Kogkos
  • A. P. Pipia
  • A. Scala
  • G. Garippa
  • A. Seimenis
Short Communication


Although cystic echinococcosis (CE) has been a recognized public health problem in Greece, molecular data are lacking regarding the types and prevalences of infecting strains of the etiological agent Echinococcus granulosus. Therefore, we investigated the prevalence of CE and determined the infecting genotypes in sheep and goats in Peloponnesus, a large region of southern Greece. Liver and lung samples were obtained from 210 sheep and 190 goats slaughtered between January and December 2005, and the number, morphology, and fertility of hydatid cysts were determined. Protoscoleces or germinal layers were collected from individual cysts (20 sheep and 20 goats), and DNA was extracted. A polymerase chain reaction (PCR)/seminested PCR system was used to distinguish the G1, G5, and G6/G7 strains, and a specific molecular diagnosis was obtained by sequencing PCR-amplified mitochondrial DNA encoding cytochrome c oxidase subunit 1 and NADH dehydrogenase I genes. The prevalence of CE was 30.4% in sheep and 14.7% in goats; fertile cysts were found in 16.2 and 7.4%, respectively. Overall, 18 of 20 sheep harbored the G1 genotype (common sheep strain), while the remaining two animals had the G3 (buffalo) strain. All 20 goats were infected with the G7 (pig) strain. These results document the prevalence of E. granulosus infection in food animals in this geographical area and reveal for the first time the presence of, at least, three parasite genotypes.


Hydatid Cyst Small Ruminant Cystic Echinococcosis Echinococcus Granulosus Cystic Echinococcosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Dr. Valerie Matarese provided scientific editing.


The experiments of this research comply with the current laws of the countries in which they were performed (Italy and Greece).


  1. Azlaf R, Dakkak A (2006) Epidemiological study of the cystic echinococcosis in Morocco. Vet Parasitol 137:83–93PubMedCrossRefGoogle Scholar
  2. Bart JM, Morariu S, Knapp J, Ilie MS, Pitulescu M, Anghel A, Cosoroaba I, Piarroux R (2006) Genetic typing of Echinococcus granulosus in Romania. Parasitol Res 98:130–137PubMedCrossRefGoogle Scholar
  3. Bowles J, McManus DP (1993a) NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol 23:969–972PubMedCrossRefGoogle Scholar
  4. Bowles J, McManus DP (1993b) Molecular variation in Echinococcus. Acta Trop 53:291–305PubMedCrossRefGoogle Scholar
  5. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54:165–174PubMedCrossRefGoogle Scholar
  6. Busi M, Snàbel V, De Liberato C, D’Amelio S (2004) Molecular genotyping of Echinococcus granulosus hydatid cysts in Italy reveals the presence of three distinct genotypes. Parassitologia 46:164Google Scholar
  7. Capuano F, Rinaldi L, Maurelli MP, Perugini AG, Veneziano V, Garippa G, Genchi C, Musella V, Cringoli G (2006) Cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains. Vet Parasitol 137:262–268PubMedCrossRefGoogle Scholar
  8. Dinkel A, Njoroge EM, Zimmermann A, Wälz M, Zeyhle E, Elmahdi IE, Mackenstedt U, Romig T (2004) A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol 34:645–653PubMedCrossRefGoogle Scholar
  9. Eckert J, Thompson RC (1997) Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Trop 64:19–34PubMedCrossRefGoogle Scholar
  10. Euzeby J (1991) The epidemiology of hydatidosis with special reference to the Mediterranean area. Parassitologia 33:25–39PubMedGoogle Scholar
  11. Garippa G, Varcasia A, Scala A (2004) Cystic echinococcosis in Italy from the 1950s to today. Parassitologia 46:387–391PubMedGoogle Scholar
  12. González LM, Daniel-Mwambete K, Montero E, Rosenzvit MC, McManus DP, Gárate T, Cuesta-Bandera C (2002) Further molecular discrimination of Spanish strains of Echinococcus granulosus. Exp Parasitol 102:46–56PubMedCrossRefGoogle Scholar
  13. Jenkins DJ, Romig T, Thompson RC (2005) Emergence/re-emergence of Echinococcus spp.: a global update. Int J Parasitol 35:1205–1219PubMedCrossRefGoogle Scholar
  14. Kamenetzky L, Gutierrez AM, Canova SG, Haag KL, Guarnera EA, Parra A, Garcia GE, Rosenzvit MC (2002) Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infect Genet Evol 2:129–136PubMedCrossRefGoogle Scholar
  15. Kedra AH, Swiderski Z, Tkach VV, Dubinský P, Pawlowski ZS, Stefaniak J, Pawlowski J (1999) Genetic analysis of Echinococcus granulosus from humans and pigs in Poland, Slovakia and Ukraine. A multicenter study. Acta Parasitol 199:248–254Google Scholar
  16. Kedra AH, Tkach VV, Swiderski Z, Pawlowski ZS, Emets A, Pawlowski J (2000) Molecular characterization of Echinococcus granulosus from a wild boar. Acta Parasitol 45:121–122Google Scholar
  17. Kyriakidis A (1995) Human echinococcosis/hydatidosis in Greece: analysis of epidemiological data for the period 1989–1993, Information Circular no. 37. WHO Mediterranean Zoonoses Control Programme, Athens, pp 7–9Google Scholar
  18. Lavikainen A, Lehtinen MJ, Meri T, Hirvelä-Koski V, Meri S (2003) Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology 127:207–215PubMedCrossRefGoogle Scholar
  19. Magambo J, Njoroge E, Zeyhle E (2006) Epidemiology and control of echinococcosis in sub-Saharan Africa. Parasitol Int 55:193–195CrossRefGoogle Scholar
  20. Mwambete D, Ponce-Gordo K, Cuesta-Bandera C (2004) Genetic identification and host range of the Spanish strains of Echinococcus granulosus. Acta Trop 91:87–93CrossRefGoogle Scholar
  21. Nakao M, McManus DP, Schantz PM, Craig PS, Ito A (2006) A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology 11:1–10Google Scholar
  22. Obwaller A, Schneider R, Walochnik J, Gollackner B, Deutz A, Janitschke K, Aspock H, Auer H (2004) Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1. Parasitology 128:569–575PubMedCrossRefGoogle Scholar
  23. Pawłowski Z, Stefaniak J (2003) The pig strain of Echinococcus granulosus in humans: a neglected issue? Trends Parasitol 19:439PubMedCrossRefGoogle Scholar
  24. Romig T, Dinkel A, Mackenstedt U (2006) The present situation of echinococcosis in Europe. Parasitol Int 55:187–191CrossRefGoogle Scholar
  25. Scala A, Varcasia A, Garippa G (2004) Cystic echinococcosis in Sardinia: the current role of sheep. Parassitologia 46:397–400PubMedGoogle Scholar
  26. Scala A, Garippa G, Varcasia A, Tranquillo VM, Genchi C (2006) Cystic echinococcosis in slaughtered sheep in Sardinia (Italy). Vet Parasitol 135:33–38PubMedCrossRefGoogle Scholar
  27. Seimenis A (2003) Overview of the epidemiological situation on echinococcosis in the Mediterranean region. Acta Trop 85:191–195PubMedCrossRefGoogle Scholar
  28. Sotiraki S, Papadopoulos E, Himonas C, Korkoliakou P (2001) Hydatidosis-echinococcosis in Greece; XXth International Congress of Hydatidology, Kusadasi, Turkey, 4–8 June 2001; Abstracts Book, p 71Google Scholar
  29. Sotiraki S, Himonas C, Korkoliakou P (2003) Hydatidosis-echinococcosis in Greece. Acta Trop 85:197–201PubMedCrossRefGoogle Scholar
  30. Thompson RCA, McManus DP (2002) Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol 18:452–457PubMedCrossRefGoogle Scholar
  31. Turceková L, Snábel V, D’Amelio S, Busi M, Dubinský P (2003) Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic. Acta Trop 85:223–229PubMedCrossRefGoogle Scholar
  32. Varcasia A, Canu S, Lightowlers MW, Scala A, Garippa G (2006) Molecular characterization of Echinococcus granulosus strains in Sardinia. Parasitol Res 98:273–277PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • A. Varcasia
    • 1
    Email author
  • S. Canu
    • 1
  • A. Kogkos
    • 1
  • A. P. Pipia
    • 1
  • A. Scala
    • 1
  • G. Garippa
    • 1
  • A. Seimenis
    • 2
  1. 1.Dipartimento di Biologia AnimaleSezione di Parassitologia e Malattie Parassitarie Università degli Studi di SassariSassariItaly
  2. 2.Mediterranean Zoonoses Control CentreWorld Health OrganizationAthensGreece

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