Parasitology Research

, Volume 101, Issue 3, pp 801–808

The use of an excreted superoxide dismutase in an ELISA and Western blotting for the diagnosis of Leishmania (Leishmania) infantum naturally infected dogs

  • C. Marín
  • S. S. Longoni
  • H. Mateo
  • J. A. de Diego
  • J. M. Alunda
  • G. Minaya
  • M. Sánchez-Moreno
Original Paper

Abstract

An excreted iron superoxide dismutase of pI 3.75 and a molecular mass of approximately 25 kDa was partially purified by QAE Sephadex ion-exchange chromatography from the in vitro culture of Leishmania (Leishmania) infantum. This enzyme was detected by enzyme-linked immunosorbent assay and Western blot of anti-L. infantum antibodies in dog serum. For the determination of the sensitivity and specificity of this protein, the results using the complete-parasite antigen fraction were taken as references. For this, 39 sera were assayed in dogs from different Spanish provinces. By Western blot, at a dilution of 1:250, 82% of the sera were positive when superoxide dismutase excreted was used as the antigen, against 56.4% positivity when the complete parasite was used as the antigen. These findings support the results of a previous study, indicating that the superoxide dismutase excreted can be useful in diagnosing L. (L.) infantum.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • C. Marín
    • 1
  • S. S. Longoni
    • 1
  • H. Mateo
    • 1
  • J. A. de Diego
    • 2
  • J. M. Alunda
    • 3
  • G. Minaya
    • 4
  • M. Sánchez-Moreno
    • 1
  1. 1.Instituto de Biotecnología, Departamento de Parasitología, Facultad de CienciasUniversidad de GranadaGranadaSpain
  2. 2.Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de MedicinaUAMMadridSpain
  3. 3.Departamento de Salud AnimalEscuela de VeterinariaMadridSpain
  4. 4.Laboratorio de Leishmaniosis y ChagasInstituto Nacional de SaludLima 11Peru

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