Parasitology Research

, Volume 101, Issue 3, pp 703–708 | Cite as

Evaluation of human IgG class and subclass antibodies to a 24 kDa antigenic component of Gnathostoma spinigerum for the serodiagnosis of gnathostomiasis

  • Porntip Laummaunwai
  • Kittisak Sawanyawisuth
  • Pewpan M. Intapan
  • Verajit Chotmongkol
  • Chaisiri Wongkham
  • Wanchai MaleewongEmail author
Original Paper


The immunoglobulin G class (total IgG) and subclass (IgG1, IgG2, IgG3, and IgG4) antibody responses to the spirurid worm Gnathostoma spinigerum were analyzed by immunoblotting technique for the antibodies’ potential use in the serodiagnosis of human gnathostomiasis. Serum samples from patients with proven gnathostomiasis and from clinically suspected cases of gnathostomiasis with migratory swelling were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The total IgG antibody to an antigenic band of approximately 24 kDa from a somatic extract of G. spinigerum advanced third-stage larvae (GS24) gave the highest sensitivity (91.6%) and 87.8% specificity. Individual IgG subclass detection had a lower sensitivity than the detection of specific total IgG antibody, but IgG4 had a slightly higher specificity (93.9%). However, for cost effectiveness, we suggest that anti GS24 total IgG is sufficient for the routine serodiagnosis of human gnathostomiasis as well as for seroepidemiological studies in developing countries.


Cysticercosis Strongyloidiasis Fascioliasis Paragonimiasis Opisthorchiasis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This investigation was supported by grants from Khon Kaen University and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University. We thank Dr. Mark Roselieb for improving the English-language presentation of the paper. The use of experimental animals complied with the current laws of Thailand wherein the experiment was performed.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Porntip Laummaunwai
    • 1
  • Kittisak Sawanyawisuth
    • 2
  • Pewpan M. Intapan
    • 1
  • Verajit Chotmongkol
    • 2
  • Chaisiri Wongkham
    • 3
  • Wanchai Maleewong
    • 1
    Email author
  1. 1.Department of Parasitology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Medicine, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  3. 3.Department of Biochemistry, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand

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