Evaluation of human IgG class and subclass antibodies to a 24 kDa antigenic component of Gnathostoma spinigerum for the serodiagnosis of gnathostomiasis
- 258 Downloads
The immunoglobulin G class (total IgG) and subclass (IgG1, IgG2, IgG3, and IgG4) antibody responses to the spirurid worm Gnathostoma spinigerum were analyzed by immunoblotting technique for the antibodies’ potential use in the serodiagnosis of human gnathostomiasis. Serum samples from patients with proven gnathostomiasis and from clinically suspected cases of gnathostomiasis with migratory swelling were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The total IgG antibody to an antigenic band of approximately 24 kDa from a somatic extract of G. spinigerum advanced third-stage larvae (GS24) gave the highest sensitivity (91.6%) and 87.8% specificity. Individual IgG subclass detection had a lower sensitivity than the detection of specific total IgG antibody, but IgG4 had a slightly higher specificity (93.9%). However, for cost effectiveness, we suggest that anti GS24 total IgG is sufficient for the routine serodiagnosis of human gnathostomiasis as well as for seroepidemiological studies in developing countries.
KeywordsCysticercosis Strongyloidiasis Fascioliasis Paragonimiasis Opisthorchiasis
This investigation was supported by grants from Khon Kaen University and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University. We thank Dr. Mark Roselieb for improving the English-language presentation of the paper. The use of experimental animals complied with the current laws of Thailand wherein the experiment was performed.
- Beaver PC, Jung RC, Cupp EW (1984) Clinical parasitology, 9th edn. Lea and Febiger, PhiladelphiaGoogle Scholar
- Chanteau S, Plichart R, Spiegel A, Martin PM, Cartel JL (1991) Diagnostic values of ELISA-IgG4 as compared to ELISA-IgG and indirect immunofluorescence, for the routine diagnosis of bancroftian filariasis in the South Pacific. Application on capillary blood collected on filter paper. Trop Med Parasitol 42:339–342PubMedGoogle Scholar
- Diaz Camacho SP, Zazueta Ramos M, Ponce Torrecillas E, Osuna Ramirez I, Castro Velazquez R, Flores Gaxiola A, Baquera Heredia J, Willms K, Akahane H, Ogata K, Nawa Y (1998) Clinical manifestations and immunodiagnosis of gnathostomiasis in Culiacan, Mexico. Am J Trop Med Hyg 59:908–915PubMedGoogle Scholar
- Elkins DB, Haswell-Elkins M, Anderson RM (1986) The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80:774–792PubMedCrossRefGoogle Scholar