Parasitology Research

, Volume 101, Issue 1, pp 219–228

First occurrence of Culicoides obsoletus-transmitted Bluetongue virus epidemic in Central Europe

  • Heinz Mehlhorn
  • Volker Walldorf
  • Sven Klimpel
  • Birgit Jahn
  • Friedhelm Jaeger
  • Josef Eschweiler
  • Bernd Hoffmann
  • Martin Beer
Original Paper

Abstract

In August 2006, Bluetongue virus disease (BTD) was detected for the first time in the Netherlands, Belgium, Germany and Northern France. Serological tests as well as reverse transcriptase polymerase chain reaction (RT-PCR) proved the occurrence of Bluetongue virus (BTV) in diseased sheep and cattle, and the virus was identified as serotype 8. Therefore, the search for possible vectors was immediately initiated in the outbreak region in Germany. Traps with automatically regulated ultraviolet light lamps were placed at two different farms with sero-positive cattle, and insect monitoring was done from August 2006 until January 2007. The caught arthropods were weekly determined, and it could be observed that midges of the dipteran family Ceratopogonidae occurred in large numbers, sometimes representing up to 40% of all individuals. The microscopical analysis of the wing morphology showed that the species (complex) Culicoides obsoletus was most abundant covering about 97% of the analysed midges. On the second place ranged C. pulicaris, while C. nubeculosus and C. festivipennis were found only as single individuals. Fed and unfed females were separated, sent to the National Reference Laboratory for Bluetongue disease (Friedrich-Loeffler-Institut, Isle of Riems, Germany) and investigated with a BTV-8-specific real-time RT-PCR. It could be demonstrated that at both farms both fed and unfed C. obsoletus were tested positive for BTV-8 genomes, while none of the other species scored positive. This finding strongly supports that the BTD-epidemic, which reached in the meantime wide regions of North Rhine–Westphalia in Germany and of the neighbouring countries with several hundreds of affected farms, is initiated by virus transmission during the blood meal of midges of the C. obsoletus complex. Since they were captured still at the 21st of December close to cattle with clinical signs, it must be feared that BTV-8 is now established in Central Europe, where it had been absent until now.

References

  1. Anonymous (1876) New Disease. Report of the South Africa cattle and sheep disease commission. Report 16:189–194Google Scholar
  2. Birley MH, Boormann JPT (1982) Estimating the survival and biting rates of haematophagous insects with particular reference to Culicoides obsoletus group in Southern England. J Anim Ecol 51:135–148CrossRefGoogle Scholar
  3. Caracappa S, Torina A, Guerico A, Vitale F, Calabro H, Purpari G, Ferratelli V, Vitali M, Mellor PS (2003) Identification of a novel Bluetongue virus vector species of Culicoides in Sicily. Vet Rec 153:71–74PubMedGoogle Scholar
  4. Carpenter S, Darpel KE (2005) Bluetongue sheep clinical. UK Vet 10:1–5Google Scholar
  5. Carpenter S, Lunt HL, Arav D, Venter GJ, Mellor PS (2006) Oral susceptibility to Bluetongue virus of Culicoides from the United Kingdom. J Med Entomol 43:73–78PubMedCrossRefGoogle Scholar
  6. De Liberato CG, Scavia R, Lorenzetti R, Scaramozzino P, Amaddeo D, Cardeti G, Sciluna M, Ferrari G, Autorino GL (2005) Identification of Culicoides obsoletus as a vector of Bluetongue virus in Central Italy. Vet Rec 156:301–304PubMedGoogle Scholar
  7. Hooper PT, Lunt RA, Stanislawek WL (1996) A trial comparing the virulence of some South African and Australian Bluetongue viruses. Aust Vet J 51:170–174Google Scholar
  8. Hutcheon D (1881) Fever of epizootic catarrh. Rep Coll Vet Surg 1880:12–15Google Scholar
  9. Hutcheon D (1902) Malarial catarrh fever of sheep. Vet Rec 14:629–633Google Scholar
  10. Jaeger F, Anczikowski I (2006) Die Blauzungenkrankheit in Deutschland—eine neue Herausforderung für die Veterinärverwaltung. Tierärztl Prax 34:381–386Google Scholar
  11. Löffler F, Frosch G (1897) Cited In: Wiesner E, Ribbeck R (eds) (1978) Wörterbuch der Veterinärmedizin. G Fischer, JenaGoogle Scholar
  12. Martini HC (1946) Med Entomology. G Fischer, JenaGoogle Scholar
  13. Mehlhorn H (2007) Encylopedic references of parasitology, 3rd edn. Springer, New YorkGoogle Scholar
  14. Mellor PS (1990) The replication of Bluetongue virus in Culicoides vectors. Curr Top Microbiol Immunol 162:143–161PubMedGoogle Scholar
  15. Mellor PS, Boormann I (1995) The transmission and geographical spread of African horse sickness and Bluetongue viruses. Ann Trop Med Parasitol 89:1–15PubMedGoogle Scholar
  16. Mellor PS, Pitzolis G (1979) Observations on breeding sites and light-trap collections of Culicoides during an outbreak of Bluetongue in Cyprus. Bull Entomol Res 69:229–234CrossRefGoogle Scholar
  17. Mellor PS, Wittmann EJ (2002) Bluetongue virus in the Mediterranean Basin (1998–2001). Vet J 164:20–37PubMedCrossRefGoogle Scholar
  18. Mellor PS, Bormann JPT, Wilkinson PJ, Martinez-Gomez F (1983) Potential vectors of Bluetongue and African horse sickness viruses in Spain. Vet Rec 112:229–230Google Scholar
  19. Mellor PS, Boormann I, Baylis M (2000) Culicoides biting midges: their role as arbovirus vectors. Annu Rev Entomol 45:307–340PubMedCrossRefGoogle Scholar
  20. Mertens PPC, Duncun R, Attoni H, Dermody TS (2004) VIII Report of the ICTV. In: Fauquet CM, Mayo MA, Maniloff I, Desselberger U, Ball LA (eds) Elsevier, London, pp 447–454Google Scholar
  21. Purse BV, Mellor PS, Rogers DJ, Samuel AR, Mertens PPC, Baylis M (2005) Climate change and the recent emerge of the Bluetongue in Europe. Nat Rev Microbiol 3:171–181PubMedCrossRefGoogle Scholar
  22. Savini G, Goffredo M, Monaco F, de Santis P, Meiswinkel R (2003) Transmission of Bluetongue virus in Italy. Vet Rec 152:119–129PubMedGoogle Scholar
  23. Verwoerd DW, Erasmus BJ (2005) Bluetongue. In Vol. Viruses. Mahy BWJ, ter Meulen V (eds) Section 4, viral diseases. Topley and Wilson’s Microbiology, Arnold, LondonGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Heinz Mehlhorn
    • 1
  • Volker Walldorf
    • 1
  • Sven Klimpel
    • 1
  • Birgit Jahn
    • 2
  • Friedhelm Jaeger
    • 3
  • Josef Eschweiler
    • 4
  • Bernd Hoffmann
    • 5
  • Martin Beer
    • 5
  1. 1.Institut für Zoologie und ParasitologieHeinrich Heine UniversitätDüsseldorfGermany
  2. 2.Bezirksregierung DüsseldorfDüsseldorfGermany
  3. 3.Ministerium für Umwelt und Naturschutz, Landwirtschaft und Verbraucherschutz NRWDüsseldorfGermany
  4. 4.Veterinäramt der Stadt AachenAachenGermany
  5. 5.Institute of Diagnostic VirologyFriedrich-Loeffler-InstitutGreifswald-Insel RiemsGermany

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