Parasitology Research

, Volume 101, Issue 2, pp 443–452 | Cite as

Antigiardial activity of Ocimum basilicum essential oil

  • Igor de Almeida
  • Daniela Sales Alviano
  • Danielle Pereira Vieira
  • Péricles Barreto Alves
  • Arie Fitzgerald Blank
  • Angela Hampshire C. S. LopesEmail author
  • Celuta Sales Alviano
  • Maria do Socorro S. Rosa
Original Paper


In this study, we investigated the effects of Ocimum basilicum essential oil on Giardia lamblia and on the modulation of the interaction of these parasites by peritoneal mouse macrophage. The essential oil (2 mg/ml) and its purified substances demonstrated antigiardial activity. Linalool (300 μg/ml), however, was able to kill 100% parasites after 1 h of incubation, which demonstrates its high antigiardial potential. Pretreatment of peritoneal mouse macrophages with 2 mg/ml essential oil dilution reduced in 79% the association index between these macrophages and G. lamblia, with a concomitant increase by 153% on nitric oxide production by the G. lamblia-ingested macrophages. The protein profiles and proteolitic activity of these parasite trophozoites, previously treated or not with 2 mg/ml essential oil or with the purified fractions, were also determined. After 1 and 2 h of incubation, proteins of lysates and culture supernatants revealed significant differences in bands patterns when compared to controls. Besides, the proteolitic activity, mainly of cysteine proteases, was clearly inhibited by the essential oil (2 mg/ml) and the purified linalool (300 μg/ml). These results suggest that, with G. lamblia, the essential oil from O. basilicum and its purified compounds, specially linalool, have a potent antimicrobial activity.


Nitric Oxide Eugenol Linalool Giardiasis Peritoneal Mouse Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Ms. Camilla M. Fortunato and Ms. Simone S. dos Santos for technical support. This work was supported by Fundação de Amparo a Pesquisa no Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Universitária José Bonifácio (FUJB), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Igor de Almeida
    • 1
    • 2
  • Daniela Sales Alviano
    • 1
  • Danielle Pereira Vieira
    • 1
  • Péricles Barreto Alves
    • 3
  • Arie Fitzgerald Blank
    • 4
  • Angela Hampshire C. S. Lopes
    • 1
    Email author
  • Celuta Sales Alviano
    • 1
  • Maria do Socorro S. Rosa
    • 1
  1. 1.Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Instituto de Química, Centro de TecnologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Departamento de QuímicaUniversidade Federal de Sergipe (UFS)São CristóvãoBrazil
  4. 4.Departamento de Engenharia AgronômicaUniversidade Federal de Sergipe (UFS)São CristóvãoBrazil

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