Parasitology Research

, Volume 101, Issue 2, pp 385–390 | Cite as

Efficacy of Lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms

Original Paper


Lagenidium giganteum is a water mold and an effective mosquito control agent with limited use due to poor survival and contamination during storage. Invert extracellular metabolites of L. giganteum is easy to produce, long shelf life, and a potential candidate in tropical climates. This fungus was grown in PYG broth in the laboratory at 25 ± 2°C, and relative humidity was maintained at 75 ± 5% for 15 ± 2 days. Filtration process of metabolites was done using Whatman filter paper, column chromatograph, and range syringe filters techniques. Then 5-ml fractions were collected and used to assay larvicidal efficacies. Larvicidal efficacies were performed against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti at five different concentrations, viz. 1.68, 1.99, 2.17, 2.30, and 2.40 ppm. And also, filtrates were assessed against four species of nontarget organisms named Daphnia pulex, Cyclopes, Lymnea auriculeta, and tadpoles of Rana tigrina with different concentrations. The mortality values were subjected by the Probit analysis. The complete mortalities that resulted from applying filtrates dosage on all instars of mosquitoes persisted for a period of 24, 48, and 72 h, respectively. The efficacies in killing instar of three important vectors and safer for nontarget organisms with good biological stability of extracellular metabolites make this a promising alternative to mycelium and conidial-based larvicides. It could be regarded as fungal-based natural larvicide for the use of vector control.


Biological Control Agent Entomopathogenic Fungus Mosquito Larva Nontarget Organism Larvicidal Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We sincerely wish to acknowledge Prof. V. G. Das, Director, Dayalbagh Educational Institute for the facilities provided and the Department of Science and technology (C-24) for providing financial support for the work to one of the authors and FIST grant of Zoology Department Awarded by DST (2003). The author is also grateful to REI Managing Committee of Dayalbagh Agra (India) for providing financial support.


  1. Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–266Google Scholar
  2. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Great BritainGoogle Scholar
  3. Gillespie AT, Claydon N (1989) The use of entomogenous fungi for pest control and the role of toxins in pathogenesis. Pestic Sci 27:203–215CrossRefGoogle Scholar
  4. Grove JF, Pople M (1980) The insecticidal activity of beauvericin and the enniatin complex. Mycopathologia 70:103–105CrossRefGoogle Scholar
  5. Hamill RL, Higgens CE, Boaz HE (1969) The structure of beauvericin; a new depsipeptide antibiotic toxic to Artemia salina. Tetrahedron Lett 49: 4255–4258CrossRefGoogle Scholar
  6. Kerwin JL, Petersen EE (1997) Fungi: oomycetes and chytridiomycetes. In: Lacey LA (ed) Manual of techniques in insect pathology, vol 5–4. Academic, New York, pp 251–268Google Scholar
  7. Kerwin JL, Washino RK (1986) Ground and aerial application of the sexual and asexual stages of Lagenidium giganteum (Oomycetes: Lagenidiales) for mosquito control. J Am Mosq Control Assoc 2:182–189PubMedGoogle Scholar
  8. Kerwin JL, Washino RK (1987) Ground and aerial application of the asexual stage of Lagenidium giganteum for control of mosquitoes associated with rice culture in the Central Valley of California. J Am Mosq Control Assoc 3:59–64PubMedGoogle Scholar
  9. Kerwin JL, Washino RK (1988) Field evaluation of Lagenidium giganteum (Oomycetes: Lagenidiales) and description of a natural epizootic involving a new isolates of fungus. J Med Entomol 25:452–460PubMedGoogle Scholar
  10. Kucera M, Samsinakova A (1968) Toxins of the entomophagous fungus Beauveria bassiana. J Invertebr Pathol 12:316–320PubMedCrossRefGoogle Scholar
  11. Legner EF (1995) Biological control of diptera of medical and veterinary importance. J Vector Ecol 20:59–120Google Scholar
  12. Matha V, Weiser J, Olejnicek J (1988) The effect of tolypin on Tolypocladium niveum crude extract against mosquito and black-fly larvae in the laboratory. Folia Parasitol 35:379–381PubMedGoogle Scholar
  13. May BA, VanderGheynst JS, Rumsey T (2006) The kinetics of Lagenidium giganteum growth in liquid and solid cultures. J Appl Microbiol 101:807–814PubMedCrossRefGoogle Scholar
  14. McCray EM Jr, Womeldorf DJ, Husbands RC, Eliason DA (1973) Laboratory observations and field tests with Lagenidium against California mosquitoes. Proc Calif Mosq Control Assoc 41:123–128Google Scholar
  15. Merriam TL, Axtell RC (1982) Evaluation of the entomogenous fungi Culicinomyces clavosporus and Coelomomyces giganteum for control of the salt marsh mosquito, Aedes taeniorhynchus. Mosq News 42:594–602Google Scholar
  16. Mohanty SS, Prakash S (2004) Extracellular metabolites of Trichophyton ajelloi against Anopheles stephensi and Culex quinquefasciatus larvae. Curr Sci 86:323–325Google Scholar
  17. Nestrud LB, Anderson RL (1994) Aquatic safety of Lagenidium giganteum: effects on freshwater fish and invertebrates. J Invertebr Pathol 64:228–233PubMedCrossRefGoogle Scholar
  18. Priyanka, Prakash S (2003) Laboratory efficacy tests for fungal metabolites of Chrysosporium tropicum against Culex quiqefasciatus. J Am Mosq Control Assoc 19:404–407PubMedGoogle Scholar
  19. Priyanka, Srivastava JN, Prakash S (2001) Chrysosporium tropicum efficacy against Anopheles stephensi larvae in the laboratory. J Am Mosq Control Assoc 17:127–130PubMedGoogle Scholar
  20. Richard JL, Bennett GA, Maracos CM (1995) Detection, identification, and surveillance of mycotoxins in cereals and other foods. Fedrip Database (NTIS) 3:45–49Google Scholar
  21. Roberts DW (1967) Some effects of Metarhizium anisopliea and its toxins on mosquito larvae. In: Van der Laan PA (ed) Insect pathology and microbiology entomology. North-Holland Publishing Company, Amsterdam, pp 1–360Google Scholar
  22. Rozendaal JA (1997) Vector control, methods for use by individuals and communities. World Health Organization, Geneva, Switzerland, pp 7–177Google Scholar
  23. Scholte E-J, Knols BGJ, Samson RA, Takken W (2004) Entomopathogenic fungi for mosquito control: a review. J Insect Sci 4:19PubMedGoogle Scholar
  24. Scholte E-J, Habi KN, Kihonda J, Takken W, Paaijmans K, Abdulla S, Killeen GF, Knols BGJ (2005) An entomopathogenic fungus for control of adult African malaria mosquitoes. Science 308:1641–1642PubMedCrossRefGoogle Scholar
  25. Suh CP, Axtell RC (1999) Lagenidium giganteum zoospores: effects of concentration, movement, light, and temperature on infection of mosquito larvae. Biol Control 15:33–38CrossRefGoogle Scholar
  26. Sur B, Bihari V, Sharma A, Joshi AK (2002) Studies on physiology, zoospore morphology and entomopathogenic potential of the aquatic oomycete: Laginidium giganteum. Mycopathologia 154:51–54PubMedCrossRefGoogle Scholar
  27. Vandergheynst J, Scher H, Guo HY, Schultz D (2006) Water-in-oil emulsions that improve the storage and delivery of the biolarvicides Lagenidium giganteum. BioControl (in press) DOI  10.1007/s10526-006-9021-9
  28. Vijayan V, Balaraman K (1991) Metabolite of fungi and actinomycetes active against mosquito larvae. Indian J Med Res 93:115–117PubMedGoogle Scholar
  29. Vyas N, Dua KK, Prakash S (2006a) Bioassay of secondary metabolite of Lagenidium giganteum on mosquito larvae for vector control. Bull Bio Sci 4:65–69Google Scholar
  30. Vyas N, Dua KK, Prakash S (2006b) Laboratory efficacy of metabolites of Lagenidium giganteum (Couch) on An. stephensi (Liston) after filterations by column chromatography. J Commun Dis 38:176–180PubMedGoogle Scholar
  31. Zizka J, Weiser J (1993) Effect of beauvericin, a toxic metabolite of Beauveria bassiana, on the ultrastructure of Cx. pipiens autogenicus larvae. Cytobios 75:13–19Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Advanced Parasitology and Vector Biotechnology Laboratory, Department of Zoology, Faculty of ScienceDayalbagh Educational InstituteDayalbaghIndia

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