Parasitology Research

, Volume 101, Issue 1, pp 111–115 | Cite as

Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran

  • N. Zia-Ali
  • A. Fazaeli
  • M. Khoramizadeh
  • D. Ajzenberg
  • M. Dardé
  • H. Keshavarz-Valian
Original Paper


Toxoplasma gondii is one of the most prevalent protozoan parasites in Iran. This study was aimed to isolate T. gondii from a variety of hosts and to genetically analyze the parasite isolates. The prevalence of T. gondii in different animal hosts was assessed in two provinces of Iran, Tehran and Mazandaran in the central and northern parts, respectively. The latex agglutination (LA) test was carried out, and antibodies were found in 24 out of 105 sheep, 5 out of 35 goats, 23 out of 45 free-ranging chickens (Gallus domesticus), 2 out of 13 ducks (Anas spp.), and two of four stray cats (Felis domesticus). T. gondii was isolated by bioassay in mice from four sheep, six chickens, one duck, two cats, and three human samples. Genotyping of these 16 isolates was performed using Multiplex PCR for five microsatellite markers and GRA6 gene sequence analysis. The results indicated that the studied isolates consisted of only two genotypes, II and III, with no evidence of type 1 or mixed genotypes.


Toxoplasmosis Tissue Cyst GRA6 Gene Mazandaran Province Mixed Genotype 
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We are indebted to N. Mohajeri, Z. Garoosi, and E. Gholami for their technical assistance. Our thanks are due to the head and staff of the Babol Health Research Station affiliated to Tehran University of Medical Sciences for their kind cooperation. We are grateful to Dr. J.P. Dubey (USDA) for the critical review and helpful discussion.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • N. Zia-Ali
    • 1
  • A. Fazaeli
    • 2
  • M. Khoramizadeh
    • 3
  • D. Ajzenberg
    • 4
  • M. Dardé
    • 4
  • H. Keshavarz-Valian
    • 3
  1. 1.Department of Medical Parasitology and Mycology, School of MedicineKerman University of Medical SciencesKermanIran
  2. 2.Department of Medical Parasitology, School of MedicineZahedan University of Medical SciencesZahedanIran
  3. 3.Department of Medical Parasitology and Mycology, School of Public Health and Institute of Health ResearchesTehran University of Medical SciencesTehranIran
  4. 4.Laboratoire de Parasitologie-Mycologie, EA3174, Faculte de MédecineLimogesFrance

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