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Parasitology Research

, Volume 100, Issue 4, pp 899–904 | Cite as

A metalloproteinase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica

  • Palmy R. JesudhasanEmail author
  • Chung-Wei Tan
  • Patrick T. K. Woo
Short Communication

Abstract

We report the identification of a Cryptobia genomic DNA gene predicted to encode a hydrophobic protein containing a zinc metalloproteinase motif, HEXXH, and hence named it a major surface proteinase 1-like (MSP-1). The MSP-1 gene was identified using universal genome walking. Southern blot analysis revealed it to be a multicopy gene. Fragments of DNA encoding a segment homologous to the HEXXH motif of MSP-1 are widely found in bacteria, yeast, parasitic protozoans, plants, and animals including humans. These results suggest that the MSP-1 may have highly conserved functions, such as in intracellular proteolysis.

Keywords

Trypanosoma Faint Band Adaptor Primer Genome Walking Metalloproteinase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to P.T.K. Woo. We thank Ms. Mandi Elridge for the continuous supply of the C. salmositica and Ms. Angela Hollis (DNA Facility, College of Biological Science) for her cooperation in sequencing the DNA.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Palmy R. Jesudhasan
    • 1
    • 2
    Email author
  • Chung-Wei Tan
    • 1
  • Patrick T. K. Woo
    • 1
  1. 1.Department of Integrative BiologyUniversity of GuelphGuelphCanada
  2. 2.Department of Poultry Science, 420 Kleberg CenterTexas A & M UniversityCollege StationUSA

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