Parasitology Research

, Volume 100, Issue 3, pp 619–624

Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland

  • Heather P. Thompson
  • James S. G. Dooley
  • John Kenny
  • Maurice McCoy
  • Colm J. Lowery
  • John E. Moore
  • Lihua Xiao
Original Paper

DOI: 10.1007/s00436-006-0305-x

Cite this article as:
Thompson, H.P., Dooley, J.S.G., Kenny, J. et al. Parasitol Res (2007) 100: 619. doi:10.1007/s00436-006-0305-x

Abstract

Cryptosporidium spp. in diarrheic calves less than 30 days old from farms across Northern Ireland were examined over a year period by microscopic, genotyping, and subtyping techniques to characterize the transmission dynamics. Cryptosporidium oocysts were detected in 291 of 779 (37.4%) animals. The prevalence rates of rotavirus, coronavirus, and Escherichia coli K99+ were lower as seen in 242 of 806 (30.0%), 46/806 (5.7%), and 16/421 (3.8%) of animals, respectively. Of the 224 Cryptosporidium-positive specimens available for molecular analysis, Cryptosporidium parvum was identified in 213 (95.1%) specimens, Cryptosporidium bovis in eight (3.6%), and Cryptosporidium deer-like genotype in three (1.3%). Sequence analysis of the 60-kDa glycoprotein gene identified 16 IIa subtypes and a new subtype family, with 120 of the 216 (55.6%) positive specimens having the subtype IIaA18G3R1. Eight of the IIa subtypes were previously seen in humans in Northern Ireland. Several subtypes were temporally or geographically unique. The genetic diversity in calves in Northern Ireland was much greater than that reported from other areas. This work demonstrates the utility of genotyping and subtyping tools in characterizing the transmission of Cryptosporidium spp. in calves and humans.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Heather P. Thompson
    • 2
  • James S. G. Dooley
    • 2
  • John Kenny
    • 3
  • Maurice McCoy
    • 3
  • Colm J. Lowery
    • 2
  • John E. Moore
    • 4
  • Lihua Xiao
    • 1
  1. 1.Division of Parasitic DiseasesCenter for Disease Control and PreventionAtlantaUSA
  2. 2.School of Biomedical Science, Faculty of Life and Health SciencesUniversity of UlsterColeraineUK
  3. 3.Department of Agriculture and Rural Development for Northern IrelandBelfastUK
  4. 4.Northern Ireland Public Health LaboratoryBelfast City HospitalBelfastUK

Personalised recommendations