Parasitology Research

, Volume 100, Issue 3, pp 633–636 | Cite as

The first detection of Neospora caninum DNA in the colostrum of infected cows

  • Bozena Moskwa
  • Katarzyna Pastusiak
  • Justyna Bien
  • Wladyslaw Cabaj
Short Communication

Abstract

Limited data is available on the vertical transmission of Neospora caninum via the colostrum. The results of our previous research revealed the presence of N. caninum DNA in the milk of seropositive cows. The aim of the present work is to demonstrate parasite DNA in colostrum samples. A polymerase chain reaction using Np21 and Np6 primers was applied to DNA isolated from the colostrum sediment in order to amplify the corresponding genomic Nc-5 region. The expected 328-bp product was obtained in colostrum samples collected both on the calving day and the day after. This is the first detection of N. caninum DNA in the colostrum of seropositive cows, and these findings implicate the possibility of N. caninum transmission through the colostrum.

Notes

Acknowledgements

The research was supported by grant No. 3 P06K 030 23 of the State Committee for Scientific Research in Warsaw, Poland. Grateful thanks to COST 854 Action.

References

  1. Anderson ML, Palmer CW, Thurmond MC (1995) Evaluation of abortions in cattle attributable to neosporosis in selected dairy herds in California. J Am Vet Med Assoc 207:1206–1210PubMedGoogle Scholar
  2. Anderson ML, Reynolds JP, Rowe JD, Sverlow KV, Packham AE, Barr BC, Conrad PA (1997) Evidence of vertical transmission of Neospora sp. infection in dairy cattle. J Am Vet Med Assoc 210:1169–1172PubMedGoogle Scholar
  3. Björkman C, Uggla A (1999) Serological diagnosis of Neospora caninum infection. Int J Parasitol 29:1497–1507PubMedCrossRefGoogle Scholar
  4. Björkman C, Holmhdahl OJM, Uggla A (1997) An indirect enzyme-linked immunoassay (ELISA) for demonstration if antibodies to Neospora caninum in serum and milk of cattle. Vet Parasitol 68:251–260PubMedCrossRefGoogle Scholar
  5. Cabaj W, Choromański L, Rodgers S, Moskwa B, Malczewski A (2000) Neospora caninum infections in aborting dairy cows in Poland. Acta Parasitol 45:113–114Google Scholar
  6. Chanlun A, Näslund K, Aiumlamaid S, Björkman C (2002) Use of bulk milk for detection of Neospora caninum infection in dairy herds in Thailand. Vet Parasitol 110:35–44PubMedCrossRefGoogle Scholar
  7. Chanlun A, Emanuelson U, Chanlun S, Aiumlamai S, Börkman C (2005) Application of repeated bulk milk testing for identification of Neospora caninum infection of Thai dairy herds. Wiad Parazytol 51 supp:31Google Scholar
  8. Davison HC, French NP, Trees AJ (1999) Herd-specific and age-specific seroprevalence of Neospora caninum in 14 British dairy herds. Vet Rec 144:547–550PubMedGoogle Scholar
  9. Davison HC, Guy CS, McGarry JW, Guy F, Willams DJL, Kelly DF, Trees AJ (2001) Experimental studies on at the transmission of Neospora caninum between cattle. Res Vet Sci 70:163–168PubMedCrossRefGoogle Scholar
  10. Dubey JP (1999) Recent advances in Neospora caninum and neosporosis. Vet Parasitol 84:349–367PubMedCrossRefGoogle Scholar
  11. Dubey JP, Lindsay DS (1996) A review of Neospora caninum and neosporosis. Vet Parasitol 67:1–59PubMedCrossRefGoogle Scholar
  12. Frössling J, Linberg A, Björkman C (2004) Evaluation of an iscom ELISA used for detection of antibodies to Neospora caninum in bulk milk. In: Programme and abstract information of the COST 854 annual meeting “Protozoal reproduction losses in farm ruminants”, Liverpool, UK, 6–9 September 2004, pp 14–15Google Scholar
  13. Guy CS, Williams DJL, Kelly DF, McGarry JW, Guy F, Bjorkman C, Smith RF, Trees AJ (2001) Neospora caninum in persistently infected, pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. Vet Rec 149:443–453PubMedGoogle Scholar
  14. Maley SW, Buxton D, Thompson KM, Schriefer ChES, Innes EA (2001) Serological analysis of calves experimentally infected with Neospora caninum: a 1-year study. Vet Parasitol 96:1–9PubMedCrossRefGoogle Scholar
  15. Moskwa B, Cabaj W, Pastusiak K, Bien J (2003) The suitability of milk in detection of Neospora caninum infection in cows. Acta Parasitol 48:138–141Google Scholar
  16. Moskwa B, Cabaj W, Pastusiak K, Bien J (2005) Current studies on neosporosis in Poland. Wiad Parazytol 51 supp:65–67Google Scholar
  17. Ortega-Mora LM, Gomez-Bautista M, Fernandez-Garcia A (2005) Neospora and neosporosis: achievements and perspectives in diagnosis. Wiad Parazytol 51 supp:63Google Scholar
  18. Packhman AE, Sverlow KW, Conrad PA, Loomis EF, Rowe JD, Anderson ML, Marsh AE, Cray C, Barr BC (1998) A modified agglutination test for Neospora caninum: development, optimalization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 5:467–473Google Scholar
  19. Pare J, Thurmond MC, Hietala SK (1996) Congenital Neospora caninum infection in dairy cattle and associated calfhood mortality. Can J Vet Res 60:133–139PubMedGoogle Scholar
  20. Peters M, Lütkefels E, Heckeroth AR, Schares G (2001) Immunohistochemical and ultrastructural evidence for Neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. Int J Parasitol 31:1144–1148PubMedCrossRefGoogle Scholar
  21. Sager H, Gloor M, Bjorkman C, Kritzner S, Gottstein B (2003) Assessment of antibody avidity in aborting cattle by somatic Neospora caninum tachyzoites antigen IgG avidity ELISA. Vet Parasitol 112:1–10PubMedCrossRefGoogle Scholar
  22. Schares G, Conraths FJ (2001) Placentophagia—an alternative way for horizontal transmission of Neospora caninum in cattle? Trends Parasitol 17:574CrossRefGoogle Scholar
  23. Schares G, Barwald A, Staubach C, Wurm R, Rauser M, Conraths FJ, Schroeder C (2004) Adaptation of a commercial ELISA for the detection of antibodies against Neospora caninum in bovine milk. Vet Parasitol 120:55–63PubMedCrossRefGoogle Scholar
  24. Schares G, Barwald A, Conraths FJ (2005) Adaptation of a surface antigen-based ELISA for the detection of antibodies against Neospora caninum in bovine milk. J Vet Med B Infect Dis Vet Public Health 52:45–48PubMedGoogle Scholar
  25. Trees AJ, Williams DJ (2005) Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol 21:558–561PubMedCrossRefGoogle Scholar
  26. Uggla A, Stenlud S, Holmadahl OJM, Jakubek EB, Thebo P, Kindahl H, Björkman C (1998) Oral Neospora caninum inoculation of neonatal calves. Int J Parasitol 28:1467–1472PubMedCrossRefGoogle Scholar
  27. Varcasia A, Capelli G, Ruiu A, Ladu M, Scala A, Bjorkman C (2006) Prevalence of Neospora caninum infection in Sardinian dairy farms (Italy) detected by iscom ELISA on tank bulk milk. Parasitol Res 98:264–267PubMedCrossRefGoogle Scholar
  28. Wiśniewski M, Cabaj W, Moskwa B, Wędrychowicz H (2002) The first detection of Neospora caninum DNA in brains of calves in Poland. Acta Vet (Beogr) 52:393–400CrossRefGoogle Scholar
  29. Wouda W, Bartels CJM, Moen AR (1999) Characteristics of Neospora caninum-associated abortion storms in dairy herds in The Netherlands (1995 to 1997) Theriogenology 52:233–245PubMedCrossRefGoogle Scholar
  30. Yamage M, Flechtner O, Gottstein B (1996) Neospora caninum: specific oligonucleotide primers for the detection of brain “cyst” DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). J Parasitol 82:272–279PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Bozena Moskwa
    • 1
  • Katarzyna Pastusiak
    • 1
  • Justyna Bien
    • 1
  • Wladyslaw Cabaj
    • 1
  1. 1.Witold Stefański Institute of Parasitology of the Polish Academy of SciencesWarsawPoland

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