Comparison of 18S ribosomal RNA gene sequences of Eurytrema coelmaticum and Eurytrema pancreaticum
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The partial 18S rRNA sequences of E. coelmaticum and E. pancreaticum were amplified using conserved primers and an evolutionary tree was constructed using Neighbor-Joining. The percent identity of Eurytrema species with other Dicrocoeliidae varied from 97.5 to 98.2, while the percent identity between the two Eurytrema species was up to 99.3. The tree showed that E. coelmaticum and E. pancreaticum were not situated in the same position, and they formed one cluster with L. collurioni. These results support a confirmation with molecular data that E. coelomaticum and E. pancreaticum are different species which apparently were not seriously questioned in the past.
This work was supported by a grant from the National Basic Research Priorities Program (973 Program), the Ministry of Science and Technology, People’s Republic of China. The authors are grateful to Prof. Zong, R.Q. for providing adults of E. coelmaticum and E. pancreaticum and to Mr. Shi, C.H. for his technical assistance.