Parasitology Research

, Volume 100, Issue 3, pp 641–644 | Cite as

Antiplasmodial activity of triterpenoid isolated from whole plants of Viola genus from South Korea

Short Communication


Species of the Viola genus, used in traditional medicines for the treatment of parasites, were collected and petroleum ether extract was obtained by sonication. The antiplasmodial activity of petroleum ether extracts was evaluated in vitro against the chloroquine-resistant FcB1 strain of Plasmodium falciparum. Bioactivity-guided fractionation of the petroleum ether extracts of the whole plants of Viola verecunda has led to the isolation of epi-oleanolic acid, a triterpenoid, displaying high antiplasmodial activity (IC50 0.18 μg/ml). This is the first report of the isolation and antiplasmodial activity of epi-oleanolic acid from this species.


  1. Akhtar N, Malik A (1993) Oleanane type triterpenes from Plumeria rubra Phytochemistry 32:1523–1525CrossRefGoogle Scholar
  2. Amer A, Mehlhorn H (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res 99(4):478–490PubMedCrossRefGoogle Scholar
  3. Carnat AP, Carnet A, Fraisse D (1998) Violarvensin, a new flavone di-C-glycoside from Viola arvensis. J Nat Prod 61:272–274PubMedCrossRefGoogle Scholar
  4. Clarkson C, Maharaj VJ, Crouch NR (2004) In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa. J Ethnopharmacol 92:177–191PubMedCrossRefGoogle Scholar
  5. Jung MJ, Yoo YC, Lee KB, Kim JB, Song KS (2004) Isolation of epi-oleanolic acid from Korean istletoe and its apoptosis-inducing activity in tumor cells. Arch Pharm Res 27:840–844PubMedGoogle Scholar
  6. Kwon HC, Lee KR, Zee OP (1997) Cytotoxic constituents of Pilea mongolica. Arch Pharm Res 20:180–183CrossRefPubMedGoogle Scholar
  7. Lee YN (1996) Flora of Korea, Kyohak-Sa, Seoul, Korea, p.126Google Scholar
  8. Lindholm P, Goransson U, Johansson S (2002) Cyclotides: a novel type of cytotoxic agents. Mol Cancer Ther 1:365–369PubMedGoogle Scholar
  9. Makler MT, Ries JM, Williams JA (1993) Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity. Am J Trop Med Hyg 48:739–741PubMedGoogle Scholar
  10. Petelot A (1952) Les plantes m´edicinales du Cambodge, du Laos et du Vietnam. In: Tome I (ed) Centre de Recherches Scientifiques et Techniques, I.D.E.O., SaigonGoogle Scholar
  11. Schuele S, Lederman RJ (2004) Long-term outcome of focal dystonia in string instrumentalists. Mov Disord 19:43–48PubMedCrossRefGoogle Scholar
  12. Trager W, Jensen JB (1976) Human malaria parasite in continuous culture. Science 193:673–675PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.National Center for Natural Products Research, School of PharmacyThe University of MississippiOxfordUSA
  2. 2.Department of Medicinal Chemistry, School of PharmacyThe University of MississippiOxfordUSA
  3. 3.Korea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea

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