Parasitology Research

, Volume 100, Issue 1, pp 187–190 | Cite as

Ultrastructural analysis of edelfosine-treated trypomastigotes and amastigotes of Trypanosoma cruzi

  • R. M. Santa-Rita
  • H. S. Barbosa
  • S. L. de Castro
Short Communication

Abstract

We have previously reported that epimastigote forms of Trypanosoma cruzi treated with the lysophospholipid analogues (LPAs) edelfosine (ET-18), ilmofosine, and miltefosine suffered alterations in plasma membrane, mitochondrion, and lipid synthesis. In this work, ET-18 induced membrane damage in trypomastigotes and amastigotes. Incubation of epimastigotes and trypomastigotes with the three LPAs led to membrane permeabilization, which was abolished by serum addition. Treatment for 24 h in culture medium interfered the with mitochondrial status of epimastigotes, with no effect in trypomastigotes, in agreement with ultrastructural data. LPAs induced alterations in the plasma membrane of the three forms of T. cruzi and in the mitochondria of epimastigotes, suggesting that these organelles are potential targets of these analogues.

Notes

Acknowledgements

We are grateful to Dr. Maurilio J. Soares for the critical reading of this manuscript and for facilitating helpful discussions. This work was sponsored by CNPq, Faperj and Fiocruz.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • R. M. Santa-Rita
    • 1
  • H. S. Barbosa
    • 1
  • S. L. de Castro
    • 1
  1. 1.Departamento de Ultra-estrutura e Biologia CelularInstituto Oswaldo CruzRio de JaneiroBrazil

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