Advertisement

Parasitology Research

, Volume 100, Issue 1, pp 25–29 | Cite as

Long-term storage of Clonorchis sinensis metacercariae in vitro

  • Shunyu Li
  • Hyoun Woo Kang
  • Min-Ho Choi
  • Sung-Tae HongEmail author
Original Paper

Abstract

Clonorchis sinensis metacercariae must often be stored for several months to meet laboratory requirements. In the present study, the authors evaluated morphological changes and infectivities of metacercariae kept in different storage solutions for 1, 3, and 6 months, i.e., PBS (phosphate buffered saline), PBS + antibiotics, PBS + iodoacetic acid (IAA), and PBS + antibiotics + IAA. Metacercariae stored in PBS containing antibiotics showed significantly higher survival rates and infectivities than those stored in PBS alone after 3 or 6 months. The metacercariae that had been stored for 6 months in solutions that were refreshed monthly had also significantly higher survival and infection rates than those stored in nonrefreshed solutions. Moreover, the addition of IAA, an inhibitor of cysteine proteinase, to these storage solutions reduced the survival and infectivity. The results of the present study confirm that the viabilities of C. sinensis metacercariae are best maintained in storage solutions containing antibiotics that are regularly refreshed.

Keywords

Ventral Sucker Cold Saline Storage Solution Iodoacetic Acid Viability Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

The present study was supported by a grant from the Medical Research Center, Seoul National University (2002).

References

  1. Li S, Chung BS, Choi MH, Hong ST (2004a) Organ-specific antigens of Clonorchis sinensis. Korean J Parasitol 42:169–174PubMedCrossRefGoogle Scholar
  2. Li S, Chung YB, Chung BS, Choi MH, Yu JR, Hong ST (2004b) The involvement of the cysteine proteases of Clonorchis sinensis metacercariae in excystment. Parasitol Res 93:36–40PubMedCrossRefGoogle Scholar
  3. Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, Fang YY (2005) Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect Dis 5:31–41PubMedCrossRefGoogle Scholar
  4. Rim HJ (2005) Clonorchiasis: an update. J Helminthol 79:269–281PubMedCrossRefGoogle Scholar
  5. Wykoff DE (1958) Studies on Clonorchis sinensis. III. The host–parasite relations in the rabbit and observations on the relative susceptibility of certain laboratory hosts. J Parasitol 44:461–466PubMedCrossRefGoogle Scholar
  6. Zhang H, Lee CH, Li S, Choi MH, Hong ST (2003) Lethal effect of ammonia on metacercariae of Clonorchis sinensis. Parasitol Res 90:421–422PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Shunyu Li
    • 1
  • Hyoun Woo Kang
    • 2
  • Min-Ho Choi
    • 2
  • Sung-Tae Hong
    • 2
    Email author
  1. 1.Department of Parasitology and Tropical MedicineYanbian UniversityYanjiPeople’s Republic of China
  2. 2.Department of Parasitology and Tropical MedicineSeoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research CenterSeoulSouth Korea

Personalised recommendations