Parasitology Research

, Volume 100, Issue 1, pp 25–29 | Cite as

Long-term storage of Clonorchis sinensis metacercariae in vitro

  • Shunyu Li
  • Hyoun Woo Kang
  • Min-Ho Choi
  • Sung-Tae HongEmail author
Original Paper


Clonorchis sinensis metacercariae must often be stored for several months to meet laboratory requirements. In the present study, the authors evaluated morphological changes and infectivities of metacercariae kept in different storage solutions for 1, 3, and 6 months, i.e., PBS (phosphate buffered saline), PBS + antibiotics, PBS + iodoacetic acid (IAA), and PBS + antibiotics + IAA. Metacercariae stored in PBS containing antibiotics showed significantly higher survival rates and infectivities than those stored in PBS alone after 3 or 6 months. The metacercariae that had been stored for 6 months in solutions that were refreshed monthly had also significantly higher survival and infection rates than those stored in nonrefreshed solutions. Moreover, the addition of IAA, an inhibitor of cysteine proteinase, to these storage solutions reduced the survival and infectivity. The results of the present study confirm that the viabilities of C. sinensis metacercariae are best maintained in storage solutions containing antibiotics that are regularly refreshed.


Ventral Sucker Cold Saline Storage Solution Iodoacetic Acid Viability Rate 
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The present study was supported by a grant from the Medical Research Center, Seoul National University (2002).


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Shunyu Li
    • 1
  • Hyoun Woo Kang
    • 2
  • Min-Ho Choi
    • 2
  • Sung-Tae Hong
    • 2
    Email author
  1. 1.Department of Parasitology and Tropical MedicineYanbian UniversityYanjiPeople’s Republic of China
  2. 2.Department of Parasitology and Tropical MedicineSeoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research CenterSeoulSouth Korea

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