Parasitology Research

, Volume 99, Issue 5, pp 597–601 | Cite as

Genetic variability of Blastocystis hominis isolates in China

  • Yiming Yan
  • Shuilian Su
  • Riyong Lai
  • Hua Liao
  • Jinhua Ye
  • Xiaobo Li
  • Xiaoting Luo
  • Guifeng Chen
Original Paper

Abstract

To determine if genetic diversity of Blastocystis hominis exists in China, 35 B. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by PCR using seven pairs of known sequenced-tagged site (STS) primers. Out of the 35 isolates, 29 were identified as one of the known genotypes, while five isolates showed two distinct genotypes, and one isolate was an unknown genotype as this was negative with all the STS primers. In this study, none of the isolates was classified as subtypes 4–7. Compared with the spectrum of human B. hominis subtypes obtained from five geographically different countries (Japan, Pakistan, Bangladesh, Germany, and Thailand), these results showed that subtype 1 was more a popular genotype (18/35) in China. In addition, two groups of the isolates from 19 asymptomatic infected individuals and those from 16 patients with intestinal symptoms were compared with the PCR-based subtype classification. The results suggest a possible relationship between subtype 1 and a pathogenic potential of this parasite.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Yiming Yan
    • 1
  • Shuilian Su
    • 2
  • Riyong Lai
    • 1
  • Hua Liao
    • 2
  • Jinhua Ye
    • 1
  • Xiaobo Li
    • 3
  • Xiaoting Luo
    • 1
  • Guifeng Chen
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyGannan Medical CollegeGanzhouPeople’s Republic of China
  2. 2.Department of Microbiology and ParasitologyGannan Medical CollegeGanzhouPeople’s Republic of China
  3. 3.Department of Preventive MedicineGannan Medical CollegeGanzhouPeople’s Republic of China

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