Genetic variability of Blastocystis hominis isolates in China
- 314 Downloads
To determine if genetic diversity of Blastocystis hominis exists in China, 35 B. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by PCR using seven pairs of known sequenced-tagged site (STS) primers. Out of the 35 isolates, 29 were identified as one of the known genotypes, while five isolates showed two distinct genotypes, and one isolate was an unknown genotype as this was negative with all the STS primers. In this study, none of the isolates was classified as subtypes 4–7. Compared with the spectrum of human B. hominis subtypes obtained from five geographically different countries (Japan, Pakistan, Bangladesh, Germany, and Thailand), these results showed that subtype 1 was more a popular genotype (18/35) in China. In addition, two groups of the isolates from 19 asymptomatic infected individuals and those from 16 patients with intestinal symptoms were compared with the PCR-based subtype classification. The results suggest a possible relationship between subtype 1 and a pathogenic potential of this parasite.
KeywordsPathogenic Potential Restriction Fragment Length Polymorphism Analysis Distinct Genotype Newborn Calf Serum Subtype Classification
This work was supported by the Provincial Education Office of Jiangxi (Grant No. 251) and Medical Research Foundation of Gannan Medical College (No. 200538).
The experiments comply with the current regulations of People’s Republic of China.
The two authors, Shuilian Su and Riyong Lai, contributed equally to this work.
- Jones WR (1946) The experimental infection of rats with Entamoeba histolytica. Ann Trop Med Parasitol 40:130Google Scholar
- Noël C, Peyronnet C, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Sogin ML, Capron M, Viscogliosi E, Zenner L (2003) Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Mol Biochem Parasitol 126:119–123PubMedCrossRefGoogle Scholar
- Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E (2005) Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 43:348–355PubMedCrossRefGoogle Scholar