Parasitology Research

, Volume 99, Issue 3, pp 297–299

Molecular evidence for Cryptosporidium infection in dogs in Central Italy

  • Annunziata Giangaspero
  • Raffaella Iorio
  • Barbara Paoletti
  • Donato Traversa
  • Gioia Capelli
Short Communication


Two hundred and forty kennel and privately owned dogs were tested for a molecular epidemiological study on Cryptosporidium infection. Genomic DNA was extracted from individual faecal samples. All the DNA extracts were analysed with a PCR assay specific for a ∼400-bp fragment internal to the gene encoding for the Cryptosporidium oocyst wall protein. The prevalence was 3.3% and it was higher in kennel dogs and in dogs with gastrointestinal symptoms. Cryptosporidiumparvum was detected by sequencing analysis in six kennel dogs and one privately owned dog, and Cryptosporidiumcanis was detected in one kennel dog. This is the first investigation on the prevalence of Cryptosporidium in Italian dogs and has pointed to the existence of genotypes that may be of public health significance.


  1. Abe N, Sawano Y, Yamada K, Kimata I, Iseki M (2002) Cryptosporidium infection in dogs in Osaka, Japan. J Vet Med Sci 64:165–168PubMedCrossRefGoogle Scholar
  2. Brandonisio O, Maggi P, Panaro MA, Lisi S, Andriola A, Acquafredda A, Angarano G (1999) Intestinal protozoa in HIV-infected patients in Apulia, south Italy. Epidemiol Infect 123:457–462PubMedCrossRefGoogle Scholar
  3. Chermette R, Blondel S (1989) Cryptosporidiose des Carnivores domestiques, resultats preliminairesen France. Bull Soc Fr Parasitol 7:31–36Google Scholar
  4. Diaz V, Campos M, Lozano J, Manas I, Gonzalez J (1996) Aspects of animal giardiosis in Granada province (southern Spain). Vet Parasitol 64:171–176PubMedCrossRefGoogle Scholar
  5. El-Ahraf AJ, Tacal JV, Sobih M, Amin M, Lawrencwe W, Wilcke BW (1991) Prevalence of cryptosporidiosis in dogs and human beings in San Bernardino County, California. J Am Vet Med Assoc 198:631–634PubMedGoogle Scholar
  6. Fayer R (2004) Cryptosporidium: a water-borne zoonotic parasite. Vet Parasitol 1:37–56CrossRefGoogle Scholar
  7. Fayer R, Trout M, Xiao L, Morgan UM, Lai AA, Dubey JP (2001) Cryptosporidium canis n. sp. from domestic dogs. J Parasitol 87:1415–1422PubMedGoogle Scholar
  8. Frost FJ, Fea E, Gilli G, Biorci F, Muller TM, Craun GF, Calderon RL (2000) Serological evidence of Cryptosporidium infections in southern Europe. Eur J Epidemiol 16:385–390PubMedCrossRefGoogle Scholar
  9. Giangaspero A, Molini U, Iorio R, Traversa D, Paoletti B, Giansante C (2005) Detection of Cryptosporidium parvum (Genotype 2) oocysts in seawater clams (Chamelea gallina) in Italy. Prev Vet Med 69:203–212PubMedGoogle Scholar
  10. Grimason AM, Smith HV, Parker JF, Jackson MH, Smith PG, Girdwood RWA (1993) Occurrence of Giardia spp. cysts and Cryptosporidium sp oocysts in faeces from public parks in the west of Scotland. Epidemiol Infect 110:641–645PubMedCrossRefGoogle Scholar
  11. Hajdusek O, Ditrich O, Slapeta J (2004) Molecular identification of Cryptosporidium spp. in animal and human hosts from the Czech Republic. Vet Parasitol 122:183–192PubMedCrossRefGoogle Scholar
  12. Hunter PR, Thompson RC (2005) The zoonotic transmission of Giardia and Cryptosporidium. Int J Parasitol 35:1181–1190PubMedCrossRefGoogle Scholar
  13. Learmonth JJ, Ionas G, Ebbett KA, Kwan ES (2004) Genetic characterization and transmission cycles of Cryptosporidium species isolated from humans in New Zealand. Appl Environ Microbiol 70:3973–3978PubMedCrossRefGoogle Scholar
  14. Morgan UM, Xiao L, Monis PT, Fall A, Irwin PJ, Fayer R, Denholm KM, Limor J, Lal AA, Thompson RCA (2000) Cryptosporidium spp. in domestics dogs: the dog genotype. Appl Environ Microbiol 66:2203–2220CrossRefGoogle Scholar
  15. Pedraza-Diaz S, Amar C, Iversen AM, Stanley PJ, McLauchlin J (2001) Unusual Cryptosporidium species recovered from human faeces: first description of Cryptosporidium felis and Cryptosporidium dog type from patients in England. J Med Microbiol 50:293–296PubMedGoogle Scholar
  16. Thompson ICR, Stevens DP, Mahmoud AAF, Warren KS (1976) Giardiasis in the mouse: an animal model. Gastroenterology 71:67–71Google Scholar
  17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  18. Traldi G (1990) Report of Cryptosporidium in an adult dog. Atti SISVet 44:1293–1294Google Scholar
  19. Traversa D, Giangaspero A, Molini U, Iorio R, Paoletti B, Otranto D, Giansante C (2004) Genotyping of Cryptosporidium isolates from Chamelea gallina clams in Italy. Appl Environ Microbiol 70:4367–4370PubMedCrossRefGoogle Scholar
  20. Uga S, Matsumura T, Ishibashi K, Yoda Y, Yatomi K, Kataoka N (1989) Cryptosporidiosis in dogs and cats in Hyogo prefecture, Japan. Jpn J Parasitol 38:139–143Google Scholar
  21. Xiao L, Limor J, Morgan UM, Sulaiman UM, Thompson RCA, Lal AA (2000) Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites. Appl Environ Microbiol 66:5499–5502PubMedCrossRefGoogle Scholar
  22. Xiao L, Bern C, Limor J, Sulaiman I, Roberts J, Checkley W, Cabrera L, Gilman RH, Lal AA (2001) Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis 183:492–497PubMedCrossRefGoogle Scholar
  23. Xiao L, Fayer R, Upton SJ (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17:72–97PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Annunziata Giangaspero
    • 1
    • 4
  • Raffaella Iorio
    • 2
  • Barbara Paoletti
    • 2
  • Donato Traversa
    • 2
  • Gioia Capelli
    • 3
  1. 1.Department of Production Science, Engineering, Mechanics and Economy, Faculty of AgricultureUniversity of FoggiaFoggiaItaly
  2. 2.Department of Comparative Biomedical Sciences, Faculty of Veterinary MedicineUniversity of TeramoTeramoItaly
  3. 3.Istituto Zooprofilattico Sperimentale delle VenezieLegnaroItaly
  4. 4.Dipartimento Pr.I.M.E.FoggiaItaly

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