Parasitology Research

, Volume 99, Issue 3, pp 297–299

Molecular evidence for Cryptosporidium infection in dogs in Central Italy

  • Annunziata Giangaspero
  • Raffaella Iorio
  • Barbara Paoletti
  • Donato Traversa
  • Gioia Capelli
Short Communication

Abstract

Two hundred and forty kennel and privately owned dogs were tested for a molecular epidemiological study on Cryptosporidium infection. Genomic DNA was extracted from individual faecal samples. All the DNA extracts were analysed with a PCR assay specific for a ∼400-bp fragment internal to the gene encoding for the Cryptosporidium oocyst wall protein. The prevalence was 3.3% and it was higher in kennel dogs and in dogs with gastrointestinal symptoms. Cryptosporidiumparvum was detected by sequencing analysis in six kennel dogs and one privately owned dog, and Cryptosporidiumcanis was detected in one kennel dog. This is the first investigation on the prevalence of Cryptosporidium in Italian dogs and has pointed to the existence of genotypes that may be of public health significance.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Annunziata Giangaspero
    • 1
    • 4
  • Raffaella Iorio
    • 2
  • Barbara Paoletti
    • 2
  • Donato Traversa
    • 2
  • Gioia Capelli
    • 3
  1. 1.Department of Production Science, Engineering, Mechanics and Economy, Faculty of AgricultureUniversity of FoggiaFoggiaItaly
  2. 2.Department of Comparative Biomedical Sciences, Faculty of Veterinary MedicineUniversity of TeramoTeramoItaly
  3. 3.Istituto Zooprofilattico Sperimentale delle VenezieLegnaroItaly
  4. 4.Dipartimento Pr.I.M.E.FoggiaItaly

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