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Parasitology Research

, Volume 99, Issue 3, pp 287–292 | Cite as

Distribution of Cryptosporidium subtypes in humans and domestic and wild ruminants in Portugal

  • Margarida Alves
  • Lihua Xiao
  • Francisco Antunes
  • Olga MatosEmail author
Original Paper

Abstract

To investigate the transmission of cryptosporidiosis in Portugal, Cryptosporidium hominis and Cryptosporidium parvum from HIV-infected patients, cattle, and wild ruminants were characterized by sequence analysis of the 60-kDa glycoprotein (GP60) gene. Fourteen subtypes within nine subtype families were identified, and three of the subtype families (If, IIb, and IId) were restricted or largely limited to Portugal. Parasites from cattle from various regions in Portugal and wild ruminants in Lisbon showed limited genetic heterogeneity (only two subtype families). All wild ruminants had the same subtype, which was also the predominant subtype in cattle all over Portugal and was found in nine HIV-infected patients in Lisbon. Two other C. parvum subtypes were only restricted to limited locations. In contrast, human parasites displayed 13 subtypes in nine subtype families, with most of the infections caused by parasites in Ib, IIa, IIc, and IId families. Two of the C. parvum subtype families (IIc and IIb) had only been found in humans. The high overall parasite diversity and high percentage of C. hominis infections attributable to Ib and C. parvum infections to IId represent unique characteristics of Cryptosporidium transmission in humans in Portugal.

Keywords

Cryptosporidiosis Cryptosporidium Parvum Zoonotic Transmission Wild Ruminant Kuwait City 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Prof. Isabel Pereira da Fonseca, Dr. Esmeralda Delgado, and Dr. Ana Mafalda Lourenço for providing bovine and wild ruminants samples. This work was supported by the Project POCTI/ESP/46369/2002 from “Fundação para a Ciência e Tecnologia”. Margarida Alves was supported by a Ph.D. grant (SFRH/BD/2898/2000) from “Fundação para a Ciência e Tecnologia”.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Margarida Alves
    • 1
  • Lihua Xiao
    • 2
  • Francisco Antunes
    • 3
  • Olga Matos
    • 1
    Email author
  1. 1.Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaLisboaPortugal
  2. 2.Division of Parasitic DiseasesCenters for Disease Control and PreventionAtlantaUSA
  3. 3.Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina (Hospital de Santa Maria)Universidade de LisboaLisboaPortugal

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