Parasitology Research

, Volume 99, Issue 4, pp 405–409 | Cite as

Visual diagnosis of Taenia saginata cysticercosis during meat inspection: is it unequivocal?

  • S. Abuseir
  • C. Epe
  • T. Schnieder
  • G. Klein
  • M. Kühne
Original Paper


A total of 267 cysts were collected from March to December 2004 from two main abattoirs in northern Germany. The cysts were classified by the usual organoleptic methods during meat inspection as Cysticercus bovis. The reported prevalence of cysticercosis in the abattoirs was 0.48 and 1.08%, respectively. The cysts were examined macroscopically for description of their morphology and constituents and classified as viable or degenerating (dead). The DNA was extracted from these cysts and subjected to polymerase chain reaction (PCR) for evaluation of the detection methods used and to make certain that the cysts did indeed belong to C. bovis, as indicated at the slaughterhouses. Two sets of primers were used with different sensitivity levels. The first, HDP1, was able to detect 200 fg of Taenia saginata DNA and 100 pg of C. bovis DNA. The other primer set, HDP2, was able to detect 1 pg of T. saginata DNA and 1 ng of C. bovis DNA. No more than 52.4% of the samples tested positive for C. bovis in the PCR using both primers, while 20% of the viable cysts and 49.2% of the degenerating cysts tested negative with both primers.


Polymerase Chain Reaction Polymerase Chain Reaction Condition Polymerase Chain Reaction Buffer Masseter Muscle Polymerase Chain Reaction Result 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Our special thanks go to Professor Lutz Gürtler, University of Greifswald, Germany, for his help in supplying conserved T. saginata, from which positive DNA samples were extracted. We are also grateful to Mr. Christoph Küsters and Mr. Thomas Scholz from the local meat inspection authorities for their help in collecting the cysts at the slaughterhouses.


  1. Berends BR, Snijders JMA, van Logtestijn JG (1993) Efficacy of current EC meat inspection procedures and some proposed revisions with respect to microbiological safety and quality assurance. Vet Rec 133:411–415PubMedGoogle Scholar
  2. Dorny P, Vercammen F, Brandt J, Vansteenkiste W, Berkvens D, Geerts S (2000) Sero-epidemiological study of Taenia saginata cysticercosis in Belgian cattle. Vet Parasitol 88:43–49CrossRefPubMedGoogle Scholar
  3. Geerts S, Kumar V, Ceulemans F, Mortelmans J (1981) Serodiagnosis of Taenia saginata cysticercosis in experimentally and naturally infected cattle by enzyme-linked immunosorbent assay. Res Vet Sci 30:288–293PubMedGoogle Scholar
  4. Gonzáles LM, Montero E, Harrison LJS, Parkhouse RME, Garate T (2000) Differential diagnosis of Taenia saginata and Taenia solium infection by PCR. J Clin Microbiol 38:737–744PubMedGoogle Scholar
  5. Gonzáles LM, Montero E, Puento S, López-Velez R, Hernández M, Sciutto E, Leslie JSH, Parkhouse RME, Gárate T (2002a) PCR tools for the differential diagnosis of Taenia saginata and Taenia solium taeniasis/cysticercosis from different geographical locations. Diagn Microbiol Infect Dis 42:243–249CrossRefPubMedGoogle Scholar
  6. Gonzáles LM, Montero E, Sciutto E, Harrison LJS, Parkhouse RME, Garate T (2002b) Differential diagnosis of Taenia saginata and Taenia solium infections: from DNA probes to polymerase chain reaction. Trans R Soc Trop Med Hyg 96(Suppl 1):243–250CrossRefGoogle Scholar
  7. Gracey J, Collins DS, Huey R (1999) Diseases caused by helminth and arthropod parasites. In: Meat hygiene, 10th edn. WB Saunders, UK, pp 635–699Google Scholar
  8. Harbers AHM (1991) Aspects of meat inspection in an integrated quality control system for slaughter pigs. Thesis. Utrecht University, 136 (quoted by Snijders and van Knapen, 1993)Google Scholar
  9. Harrison LJS (1996) Epidemiology and control of Taenia saginata cysticercosis. Centre for Tropical Veterinary Medicine, University of Edinburgh, UK, R 5580Google Scholar
  10. Harrison LJS, Delgado J, Parckhouse RME (1990) Differential diagnosis of Taenia saginata and Taenia solium with DNA probes. Parasitology 100:459–461PubMedCrossRefGoogle Scholar
  11. Hughes G, Hoque M, Tewes MS, Wright SH, Harrison LJS (1993) Sero-epidemiological study of Taenia saginata cysticercosis in Swaziland. Res Vet Sci 55:287–291PubMedGoogle Scholar
  12. Minozzo JC, Gusso RLF, Castro EA, Lago O, Soccol VT (2002) Experimental bovine infection with Taenia saginata eggs: recovery rates and cysticerci location. Braz Arch Biol Technol 45:451–455CrossRefGoogle Scholar
  13. Ogunremi O, MacDonald G, Geerts S, Brandt J (2004) Diagnosis of Taenia saginata cysticercosis by immunohistochemical test on formalin-fixed and paraffin-embedded bovine lesions. J Vet Diagn Invest 16:438–441PubMedGoogle Scholar
  14. Onyango-Abuge JA, Hughes G, Opicha M, Nginyi KM, Rugutt MK, Wright SH, Harrison LJ (1996) Diagnosis of Taenia saginata cysticercosis in Kenyan cattle by antibody and antigen ELISA. Vet Parasitol 61:221–230CrossRefPubMedGoogle Scholar
  15. Rommel M, Eckert J, Kutzer E, Körting W, Schnieder T (2000) Taenia saginata-Zystizerkose. In: Veterinärmedizinische Parasitologie. Barey Buchverlag, Berlin, Germany, pp 218–221Google Scholar
  16. SCVPH (Scientific Committee on Veterinary Measures Relating to Public Health) (2000) Opinion on the control of taeniosis/cysticercosis in man and animals, adopted on 27–28 September. (
  17. SCVPH (Scientific Committee on Veterinary Measures relating to Public Health) (2003) Opinion on revision of meat inspection in veal calves, adopted on 14-15 April. (
  18. Urquhart GM, Armor J, Duncan JL, Dunn AM, Jennings FW (1988) Veterinary Parasitology. Longman Scientific and Technical, UK, pp 119–120Google Scholar
  19. van der Logt PB, Gottstein B (2000) Unidentified parasitic cysts in cattle. Vet Rec 146(21):610–612PubMedGoogle Scholar
  20. van Knapen F, Buys J (1985) Tapeworms in the Netherlands. Tijdschr Diergeneeskd 110:761–770PubMedGoogle Scholar
  21. Wanzala W, Onyango-Abuji JA, Kang’ethe EK, Ochanda H, Harrison LJ (2002) Serodiagnosis of bovine cysticercosis by detecting live Taenia saginata cysts using a monoclonal antibody-based antigen ELISA. J S Afr Vet Assn 73:201–206Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • S. Abuseir
    • 1
  • C. Epe
    • 2
  • T. Schnieder
    • 2
  • G. Klein
    • 1
  • M. Kühne
    • 3
  1. 1.Centre for Food Science, Institute for Food Quality and SafetyUniversity of Veterinary Medicine HannoverHannoverGermany
  2. 2.Centre for Infectious Medicine, Institute for ParasitologyUniversity of Veterinary Medicine HannoverHannoverGermany
  3. 3.Lower Saxony Federal State Office for Consumer Protection and Food SafetyHead of Department 5OldenburgGermany

Personalised recommendations