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Parasitology Research

, Volume 99, Issue 4, pp 313–316 | Cite as

Giardia lamblia cysteine proteases

  • Kelly N. DuBoisEmail author
  • Marla Abodeely
  • Mohammed Sajid
  • Juan C. Engel
  • James H. McKerrow
Review

Cysteine proteases catalyze the cleavage of amide linkages in macromolecular proteins and oligomeric peptides. Cysteine proteases are classified into clans. The most abundant, clan CA, are those proteases resembling papain, the first clearly recognized cysteine protease (Barrett et al. 2004). Within clan CA, proteases are further divided into families, of which family C1 (represented by cathepsins B and L) is of interest in this review. The cathepsins function in a variety of cellular processes in eukaryotic cells. The cathepsins are synthesized as zymogens, and are activated by proteolytic removal of the N-terminal propeptide. The pro-region of the protease can act as an intramolecular chaperone to assist in protein folding, an inhibitor regulating protease activity, and a trafficking signal that targets the protease to its destination (Turk et al. 2002). In higher eukaryotes, the destination and location of activity for many cathepsins is the lysosome. These cathepsins, such as C...

Keywords

Cysteine Protease Cysteine Protease Activity Fluorogenic Peptide Cysteine Protease Gene Giardia Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Barrett AJ, Rawlings ND et al (2004) Handbook of proteolytic enzymes. Academic, San DiegoGoogle Scholar
  2. Illy C, Quraishi O et al (1997) Role of the occluding loop in cathepsin B activity. J Biol Chem 272(2):202–1197Google Scholar
  3. McArthur AG, Morrison HG et al (2000) The Giardia genome project database. FEMS Microbiol Lett 189(2):271–273CrossRefPubMedGoogle Scholar
  4. Sajid M, McKerrow JH (2002) Cysteine proteases of parasitic organisms. Mol Biochem Parasitol 120(1):1–21CrossRefPubMedGoogle Scholar
  5. Touz MC, Nores MJ et al (2002) The activity of a developmentally regulated cysteine proteinase is required for cyst wall formation in the primitive eukaryote Giardia lamblia. J Biol Chem 277(10):8474–8481CrossRefPubMedGoogle Scholar
  6. Turk V, Turk B et al (2002) Lysosomal cathepsins: structure, role in antigen processing and presentation, and cancer. Adv Enzyme Regul 42:285–303CrossRefPubMedGoogle Scholar
  7. Ward W, Alvarado L et al (1997) A primitive enzyme for a primitive cell: the protease required for excystation of Giardia. Cell 89(3):437–444CrossRefPubMedGoogle Scholar
  8. Yee J, Nash TE (1995) Transient transfection and expression of firefly luciferase in Giardia lamblia. Proc Natl Acad Sci U S A 92(12):5615–5619PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kelly N. DuBois
    • 1
    • 2
    • 3
    Email author
  • Marla Abodeely
    • 1
    • 2
  • Mohammed Sajid
    • 1
  • Juan C. Engel
    • 1
  • James H. McKerrow
    • 1
  1. 1.Department of Pathology and the Sandler Center for Basic Research in Parasitic DiseasesUniversity of CaliforniaSan FranciscoUSA
  2. 2.Biomedical Sciences Graduate ProgramUniversity of CaliforniaSan FranciscoUSA
  3. 3.University of California, San FranciscoSan FranciscoUSA

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